DNA (cytosine-5) methyltransferase (mouse), Dnmt1
DNA (cytosine-5) methyltransferase (mouse), Dnmt1
General information
| Cat. No. | ：FNK-10-201 |
| Size | ：300 Units |
| Host Species | ：Rabbit |
| Definition of specific activity | ：1 unit is defined as the amount of the enzyme that transfer 1 pmole of methyl group to poly dI-dC substrate during 30 minutes at 37℃ |
| Specific activity | ：17 units/ul |
| Quality Assurance | ：Greater than 95% protein determined by SDS-PAGE (CBB staining) (Fig.1) |
| Reaction Conditions | ：Incubate in 1 x Dnmt1 Reaction Buffer (20mM Tris-HCl, pH7.4, 0.5 mM EDTA, 0.2 mM DTT, 5% glycerol) with 10μM S-adenosylmethionine (SAM) at 37℃ |
| Application | ：1. In vitro metylation of cytosine residues in hemimethylated DNA at 5’….CG…3’. (ref. 2) ：2. Antigen for anti-mammalian Dnmt1 antibodies. |
| Storage | ：-80℃ |
| Form | ：0.5mg protein/ml in 0.2M NaCl, 10mM HEPES (pH 7.4), 50% glycerol |
| Data Link | ：UniProtKB/Swiss-Prot P13864 (DNMT1_MOUSE) |
Description
DNA methylation is significant for the epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development. Abberant methylation patterns are associated with certain human tumors and developmental abnormalities.
In vertebrates, there are two types of DNA methyltransferase activities: de novo and maintenance types. Two DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation of methylation patterns at an early stage of embryogenesis (de novo-type), while Dnmt1 is responsible for the maintenance of methylation patterns during replication. Dnmt1 favors to methylate the hemimethylated DNA and preferentially methylates one strand of the double-stranded DNA during its processive methylation.
This product, mouse Dnmt1 deleting the N-terminal 290 amino acid residues, was expressed using a baculovirus expression system* and purified by Prof. S. Tajima and Dr. I. Suetake of Osaka University (ref.1).
*Since contamination of a trace of recombinant baculovirus in the purified Dnmt1 product can not be excluded, this product should be treated by the regulation based on Cartagena Protocol on Biodiversity The product or the container should be sterilized by autoclave, 121℃ for 20 min for disposal.
Fig.1 SDS-polyacrylamide gel electrophoresis of recombinant Dnmt1
Reagents Supplied with Enzyme
Dnmt1 Reaction Buffer (5 x)
20mM S-adenosylmethionine (SAM) which was purified by chromatography from the commercial reagent and dissolved in H2O
Note
SAM is very unstable. Store at -80℃ and use it within 6 months.
References
This product was used in ref.1 and 2.
- Takeshita K et al “Structural insight into maintenance methylation by mouse DNA methyltransferase 1 (Dnmt1).” PNAS 108:9055-9 (８2011. PMID: 21518897)
- Vilkaitis G et al (2005) “Processive methylation of hemimethylated CpG sites by mouse Dnmt1 DNA methyl-transferase.” J Biol Chem 280: 64-72 PMID：15509558
Aliases for DNMT1 Gene
- DNA Methyltransferase 1 2 3 5
- CXXC9 2 3 4
- MCMT 2 3 4
- DNA (Cytosine-5-)-Methyltransferase 1 2 3
- DNA (Cytosine-5)-Methyltransferase 1 3 4
- CXXC-Type Zinc Finger Protein 9 3 4
- DNA Methyltransferase HsaI 3 4
- DNA MTase HsaI 3 4
- EC 2.1.1.37 4 51
- M.HsaI 3 4
- DNMT 3 4
- AIM 3 4
- ADCADN 3
- HSN1E 3
- DNMT1 5
- Dnmt1 4
