No More Sales-Anti-Dnmt3b antibody, affinity-purified (rabbit polyclonal), CHIP grade, 20 ug

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Anti-Dnmt3b antibody, affinity-purified (rabbit polyclonal), CHIP grade, 20 ug


inactived-FNK-70-205

Researchers can use these antibodies and antigens for studying epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development. Abberant methylation patterns are associated with certain human tumors and developmental abnormalities.

These antibodies have excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunofluorescence research with optimization.  

General information 

Description 

Dnmt3b (DNA (cytosine-5-)-methyltransferase 3b) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics). Dnmt3b is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facialanomalies (ICF) syndrome. 

This antibody was preparedand characterizedby Prof. S. Tajima of Osaka Univ. andused in Ref. 2~4.
 

Fig.1 Western blotting of Dnmt3b. The amounts of Dnmt3b in mouse embryos at the stages of E7.0-E16.5 were examined by Western blotting. The embryos were solubilized by sonication or homogenization in the presence of 0.1% SDS. The dissected embryo (10 ug protein) at each stage was subjected to Western blotting with this antibody. Dnmt3b was highly expressed in E7.0 embryo but decreased thereafter and was below the detection level after E10.5. cDNAs of Dnmt3b isoforms (3b1, 3b2, and 3b3) were transiently expressed in 293T cells and these isoforms were also detectable with this antibody.

Fig.2 Expression of Dnmt3b in mouse embyos was examined by immuno-fluorescence staining. Mouse embryos at E4.5, 5.5 and 7.0 were fixed in cold acetone and stained with this antibody or DAPI (blue). The antibody was detected with anti-rabbit IgG conjugated with ALEXA568 (red). The ICM (the inner cell mass) and trophectoderm (T) are indicated by arrows andarrowheads, respectively. The epiblast(E), and the embryonic ectoderm (EE) and extraembryonic region (EX) are indicated by square brackets. Dnmt3b existed at E4.5-7.0, in ICM atE4.5,the epiblastat E5.5, and the embryonic ectodermat E7.0.

References
This antibody was used for Western blotting and immunohistochemistry in Ref. 2~4.

1. Rhee I et al “DNMT1 and DNMT3b cooperate to silence genes in human cancer cells” Nature 416: 552-6 (2002) PMID: 11932749
2. Aoki A et al “Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases” Nucleic Acids Research 29: 3506-3512 (2001) PMID: 11522819
3. Watanabe D et al “Stage- and cell-specific expression of Dnmt3a and Dnmt3b during embryogenesis” Mechanisms of Development 118: 187-190 (2002) PMID: 12351185
4. Sakai Y et al “Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos” Gene ExpressionPatterns 5: 231-237 (2004) PMID: 15567719

• DNA Methyltransferase 3 Beta ^2 3 5
• DNA (Cytosine-5-)-Methyltransferase 3 Beta ^2 3
• DNA (Cytosine-5)-Methyltransferase 3B ^3 4
• DNA Methyltransferase HsaIIIB ^3 4
• DNA MTase HsaIIIB ^3 4
• EC 2.1.1.37 ^4 51
• M.HsaIIIB ^3 4
• DNA Cytosine-5--Methyltransferase 3 Beta ³
• DNMT3B ⁵
• Dnmt3b ⁴
• ICF1 ³
• ICF ³