Taq DNA ligase

Taq DNA ligase 

Cat. No.: SB-G3459-2000U
Size: 2000U


Description

Taq DNA ligase is derived from Thermus thermophilus  HB8 ligase and is recombinantly expressed in Escherichia coli. It is a thermostable DNA ligase that can catalyze the formation of phosphate diphosphate between the 5'-phosphate and 3'-hydroxyl of two adjacent DNA strands. ester bond. Taq DNA Ligase uses NAD as a cofactor, which is active at temperatures from 37°C–75°C.

• Source : derived from Thermus thermophilus  HB8 is recombinantly expressed in Escherichia coli.
• Definition of enzyme activity: 1 enzyme is defined as the amount of enzyme required to ligate 50% of 1 μg of BstEII-digested λDNA fragments (12 bp sticky ends) in a 50 μL reaction system at 45°C for 15 minutes living unit.
  Purity and concentration: SDS-PAGE detection purity ≥ 95%; endogenous nucleic acid residue < 1 pg/μL (qPCR detection); 40 U/μL.
• Enzyme Storage Buffer: 10 mM Tris-HCl, 50 mM KCL, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4.
• 10x Taq Reaction Buffer: 200 mM Tris-HCl, 250 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM NAD+, 100 mM DTT, 1% Triton® X-100, pH 7.6.

Storage and transportation

Transport in wet ice packs; store at -20°C, valid for 12 months.

Product Contents