Mainly used in isothermal amplification reactions, such as LAMP (Loop-mediated isothermal amplification), RCA (Rolling-circle amplification), etc
Source : The large fragment derived from Bacillus stearothermophilus DNA polymerase I
Purity and concentration : SDS-PAGE detection purity ≥ 95%
1600 U
DNA polymerase I (E. coli)
SB-G3478-500U
In the presence of templates and primers (DNA or RNA), dNTPs are used as substrates to synthesize DNA complementary to the template along the 5'-3' direction. The enzyme also retains double-strand specific 5'-3' exonuclease activity and single-strand specific 3'-5' exonuclease activity
Source : derived from Escherichia coli
Purity and concentration: SDS-PAGE detection purity ≥ 95%
500 U
DNA polymerase I, Large (Klenow) Fragment
SB-G3457-100U
Mainly used for the blunt end of the 5' or 3' protruding end of double-stranded DNA, 5' end labeling, Sanger dideoxy method for DNA sequencing, cDNA second-strand synthesis, site-directed mutagenesis, etc
Source : derived from Escherichia coli
Purity and concentration: SDS-PAGE detection purity ≥ 95%
100 U
SmartDye™, Terminator v3.1 Cycle Sequencing Kit
Cat No.
No. of Reactions
Includes*
Link
BM-PR6000-24
24 rxns
200µl dye, 1ml 5x Buffer
BM-PR6000-100
100 rxns
800µl dye, 1ml 5x Buffer
BM-PR6000-1K
1,000 rxns
4 x 2ml dye, 12ml 5x Buffer
BM-PR6000-5K
5,000 rxns
2 x 20ml dye, 12ml 5x Buffer
BM-PR6000-25K
25,000 rxns
10 x 20ml dye, 12ml 5x Buffer
* Kits include SmartDye™, 5X reaction buffer, control and primer.