Routine Cloning & Protein Expression
Product#: RoutineCloning&ProteinExpression
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Description
Sonic™ Competent Cells allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the Sonic™ strain is a BL21-derivative that features complete deletions of the endA and recA genes, eliminating degradation and recombination of DNA.
For optimal results, recommended for use in combination with Yeastern’s proprietary T&A™ expression vector, which will combine transformation, cloning and protein expression into one single step.
Features
- Fast: Only 4-6hrs culture time required to isolates plasmid DNA
- Stable: Lack of ompT and Ion proteases to stabilize synthesized protein; deletion of endA and recA eliminates DNA degradation and recombination
- High efficiency and protein yield: 1 x 108 cfu/ug
- Simple to use: clone and express with the same strain, no more sub-cloning required!
- protein expression/ toxic protein expression
- preservation of the PCR product
- preservation of the expression plasmid
Genotype: F– ompT hsdsB (rB–mB–) gal dcm (DE3) ΔendA ΔrecA
| Image | Cat.No: | Control Plasmid | Control Efficiency | Storage | Quantity | Link |
 |
YBB-FYE907-Sonic-5VL | 5 μl (10-4 μg/μl) | >1 x 108 cfu/μg | -70°C ~ -80°C | 5 |  |
| YBB-FYE907-Sonic-20VL | 20 | |
