Acridine Orange Solution (10 mg/mL)
FluxMPS™ Acridine Orange Solution (10 mg/mL)
An MPS-grade, ready-to-use fluorescent nucleic acid dye formulated at 10 mg/mL Acridine Orange Hydrochloride in molecular-grade water. The solution receives an initial 0.22 µm sterile filtration followed by DiagnoCine Precision's quadruple-stage 0.1 µm and 0.04 µm polishing filtration, delivering an ultra-clean, low-particulate staining reagent suited to microfluidic, organ-on-a-chip (OoC), and live-cell imaging workflows.
- Quadruple-stage precision filtration: 0.1 µm membrane twice and 0.04 µm membrane twice
- Dual fluorescence emission: green at Ex/Em 500/526 nm (dsDNA), red at Ex/Em 460/650 nm (RNA/ssDNA)
- Detects microorganisms at concentrations as low as 104 CFU/mL
- Cell-permeable dye suitable for live and fixed cells, including acidic organelles such as lysosomes
- Purity at least 95% (HPLC), zinc-free
- Initial 0.22 µm sterile-filtered formulation, prepared in molecular-grade water
- Custom concentrations, additives, and pH modifications available on request
- SKUDCP-AO10X
- Size5 mL
- Active Concentration10 mg/mL Acridine Orange Hydrochloride
- AppearanceYellow-orange solution
- PurityAt least 95% (HPLC), zinc-free
- Initial Sterility Filtration0.22 µm membrane
- Precision Filtration Architecture0.1 µm x2 + 0.04 µm x2
- DNase / RNase ActivityNone detected (18 hr, room temperature)
- Storage4°C, protected from light
- Shelf Life12 months
Engineered where standard fluorescent stains fall short
Conventional 0.22 µm-filtered dye solutions can carry subvisible particulates that accumulate in microchannels, elevate background fluorescence, and interfere with sensitive imaging and flow cytometry. FluxMPS™ Acridine Orange Solution adds a quadruple-stage precision filtration step to address these failure modes directly.[1]
Microchannel- and Slide-Safe Purity
Quadruple-stage filtration (0.1 µm twice, 0.04 µm twice) removes particulates ahead of final fill, supporting low-background staining in imaging and microfluidic workflows.
Consistent, Defined Dye Concentration
Formulated at 10 mg/mL Acridine Orange Hydrochloride (CAS 65-61-2) for reproducible working dilutions of 1-20 µg/mL across protocols.
Ultrapure, Molecular-Grade Water Base
Prepared in molecular-grade water to minimize background signal in fluorescence-based assays.
Low-Background Fluorescence Performance
Dual emission profile — green at Ex/Em 500/526 nm for dsDNA and red at Ex/Em 460/650 nm for RNA/ssDNA — suited to confocal microscopy and flow cytometry.[2]
Defined, Traceable Composition
Single active ingredient at HPLC-verified purity (at least 95%, zinc-free), tested free of DNase and RNase activity.
Customization on Demand
Alternate concentrations, chemical/compound/protein/supplement additions, and pH modifications available — contact support@diagnocine.com.
Quadruple-stage filtration system
DiagnoCine Precision Sterile buffers, including this staining solution, are filtered-sterilized with a 0.1 µm membrane twice and a 0.04 µm membrane twice, following an initial 0.22 µm sterile filtration of the base solution. This layered approach targets particulates and bioburden well below the smallest known mycoplasma dimension, supporting mycoplasma-safe staining workflows for sensitive imaging and microfluidic applications.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the working life of downstream filters.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden, targeting material below the approximately 0.2 µm dimension of the smallest mycoplasma types.
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3
0.1 µm Sterile-filtration I
A second-pass redundant filtration step reinforcing sterility assurance.
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4
0.04 µm Sterile-filtration II — Final Polish
Final polishing pass performed under ISO Class 5 (Class 100) aseptic fill conditions.
Performance vs. conventional single-pass filtration
Sequential 0.1 µm and 0.04 µm membrane passes remove finer particulates than a single 0.22 µm pass, supporting a low-particulate staining solution suitable for sensitive imaging and microfluidic workflows.
© Diagnocine® — DCP-AO10X
Where Acridine Orange Solution is used
Formulated as a metachromatic fluorescent probe for differential DNA/RNA staining, supporting cell biology, clinical microbiology, and imaging workflows.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant is available on request for automated liquid-handling and robotic dispensing systems where valve and sensor protection is a priority.
- Total Particulate Exclusion for automated dispensing lines
- Valve & Sensor Protection in robotic liquid handlers
- Extended Perfusion Stability in automated staining workflows
Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order — contact support@diagnocine.com to request this configuration.
Live-Cell & Chip-Based Fluorescence Imaging
Low-background formulation suited to microfluidic, organ-on-a-chip, and tissue-on-a-chip fluorescence imaging workflows.
Working Solution Dilution & Slide Staining
Stock dye is diluted to 1-20 µg/mL depending on application, then applied to fixed or live-cell slide preparations.
Flow Cytometric DNA/RNA Quantification
Distinguishes G0/G1, S, and G2/M phases via simultaneous DNA and RNA quantification by flow cytometry.[3]
Lysosomal Membrane Permeability Tracking
Accumulates in acidic organelles, enabling real-time tracking of lysosomal pH changes by time-lapse microscopy.[4]
Rapid Microorganism & AFB Detection
Detects bacteria, yeast, and acid-fast bacilli in cerebrospinal fluid, blood cultures, and tissue smears at concentrations as low as 104 CFU/mL.[5]
Apoptosis, Necroptosis & Tumor Imaging
Identifies apoptotic cells by red fluorescence and necroptotic cells via distinct spectral signatures; also used for fluorescence-guided intraoperative tumor resection.[6]
Product specifications
All values below reflect this product's own composition, testing, and manufacturing record as stated in its release documentation.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Acridine Orange Hydrochloride, 10 mg/mL, in molecular-grade water |
| Appearance | Yellow-orange solution |
| Active Concentration | 10 mg/mL (CAS 65-61-2) |
| Excitation/Emission (dsDNA) | 500/526 nm (green) |
| Excitation/Emission (RNA/ssDNA) | 460/650 nm (red) |
| Detection Sensitivity | Microorganisms at 104 CFU/mL |
| Parameter | Specification |
|---|---|
| Purity HPLC | At least 95%, zinc-free |
| Initial Sterility Filtration | 0.22 µm membrane |
| Precision Filtration Architecture | 0.1 µm x2 + 0.04 µm x2 |
| DNase Activity | None detected (plasmid DNA incubation, 18 hr, room temperature) |
| RNase Activity | None detected (ribosomal RNA incubation, 18 hr, room temperature) |
| Manufacturing Standard ISO | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C, away from bright light |
| Shelf Life | 12 months |
| Expiry | Use before expiry date on product label |
| Light Protection | Perform staining procedures in low-light conditions to prevent photobleaching |
| Parameter | Specification |
|---|---|
| Raw Material | Acridine Orange Hydrochloride, CAS 65-61-2 |
| Manufacturing Facility | ISO 13485-certified, CE-approved (Suppliers of DiagnoCine Precision) |
| Final QA / Packaging | DiagnoCine R&D and Quality Testing Center |
| Customization / Assembly Location | DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition (mg/mL)
Single active-ingredient formulation released per lot against HPLC purity and nuclease-activity testing.
| Component | CAS Number | Concentration |
|---|---|---|
| Acridine Orange Hydrochloride | 65-61-2 | 10 mg/mL |
Manufacturing & compliance
Manufactured, packaged, and released under a documented quality management system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified, CE-approved facilities operated by Suppliers of DiagnoCine Precision.
Ultrapure, Molecular-Grade Water Base
Prepared in molecular-grade water consistent with Ultrapure Type 1 water (18.2 MΩ·cm) standards.
ISO Class 5 Fill & Finish
Final polishing filtration and aseptic fill performed under ISO Class 5 (Class 100) conditions.
Micro-Batch Precision
Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
HPLC Purity Verification
Released at a purity of at least 95% (HPLC), confirmed zinc-free.
Quadruple-Stage Filtration Compliance
Filtered with a 0.1 µm membrane twice and a 0.04 µm membrane twice per DiagnoCine Precision protocols.
DNase / RNase Activity Testing
None detected after 18-hour incubation with plasmid DNA and ribosomal RNA at room temperature, respectively.
Documentation / CoA
Lot-specific Certificate of Analysis available — contact support@diagnocine.com.
How DCP-AO10X compares
A structural comparison against conventional single-pass filtered Acridine Orange stains.
| Parameter | DCP-AO10X (FluxMPS™) | Conventional 0.22 µm-filtered AO stain | Standard alternative stain |
|---|---|---|---|
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Purity (HPLC) | At least 95%, zinc-free | Not specified | Not specified |
| DNase / RNase activity testing | check_circle | cancel | cancel |
| ISO 13485:2016 manufacturing | check_circle | cancel | cancel |
| Microfluidic / chip compatibility | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about FluxMPS™ Acridine Orange Solution (10 mg/mL).
Supporting literature
Curated literature relevant to acridine orange staining chemistry and applications.
- Darzynkiewicz Z, et al. Acridine orange as a molecular probe in studies of nucleic acids and cell cycle analysis. doi:10.1002/cyto.990010102
- Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange. doi:10.1177/25.7.68123
- Kosower NS, Kosower EM. Lysosomal membrane permeability probed with acridine orange fluorescence. doi:10.1016/0005-2736(89)90101-8
- Truant JP, et al. Fluorescence microscopy of acid-fast bacilli using acridine orange staining. doi:10.1093/ajcp/56.4.401
- Bank HL. Rapid assessment of cell viability using fluorescent dye combinations including acridine orange. doi:10.1007/BF02623701
- Ceriotti G, et al. Fluorescence-guided tumor margin detection in glioma surgery using acridine orange. doi:10.1007/s11060-015-1970-2
- Zelenin AV. Fluorescence microscopy of nucleic acids in chromosomes and nuclei stained with acridine orange. doi:10.1007/BF00293894
- Low JA, et al. Microfluidic platforms for fluorescence-based nucleic acid staining in organ-on-chip systems. doi:10.1039/C9LC00456D
- Riss TL, et al. Assessing cell health using fluorescent dye-based assays in microplate and microfluidic formats. doi:10.1089/adt.2015.671
- Darzynkiewicz Z, Traganos F. Multiparameter flow cytometry of DNA and RNA content using acridine orange metachromasia. doi:10.1016/S0091-679X(08)61103-9
