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β-1,4-Mannanase TT1, Thermostable

Product#: FNK-MNN-75-01

$301.48

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β-1,4-Mannanase TT1, Thermostable

Cat. No. FNK-MNN-75-01
Lot No. 1-H201T
Protein conc. 2.1 mg/ml
Volume 10 ml
Form 20 mM Tris-HCl(pH 7.5), 50 mM NaCl
Storage -20 ºC
Activity 323 U/ml
Notes For research use only

EC No. 3.2.1.78
Substrate mannan
Reaction product mannno-origosaccaharides mannose
Reaction temperature ~100℃
Reaction pH 4.5~7.0

Description

Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated

Activity measurement

The enzyme was mixed with the pre-warmed substrate solution (0.5% ß-1,4-D-mannan in 50 mM sodium acetate buffer (pH6.0)). After incubation at 90 oC for 5 min, the reducing power was measured by the modified DNS method. One unit is defined as the amount of enzyme producing reducing power equivalent to 1 µmol of glucose per min.