EZgeneTM 96-well yeast plasmid kit is designed for rapid and reliable isolation of high-quality plasmid DNA from yeast cultures in 96-well format. Utilizing the reversible nucleic acid-binding properties of our matrix, the plasmid DNA is bound to the matrix while proteins and other unwanted impurities are eliminated by wash buffer. Pure DNA is then eluted. Purified DNA can be directly used in downstream applications such as PCR, restriction digestion, and Southern Blot.
The Yeast Plasmid Mini Kit combines the power of spin column technology with the lyticase and alkaline-SDS lysis of yeast cells to yield high quality plasmid DNA in less than 90 minutes. The mini spin columns facilitate the binding, washing, and elution steps, thus enabling multiple samples to be processed simultaneously. The actual plasmid yields depend on copy numbers, yeast strain, and conditions of growth. Because of low copy numbers, the maximum yield from 1 mL yeast culture is around 0.2 -1 µg.
Storage and Stability:
All components are guaranteed for 24 months from the date of purchase. The Buffer YP I/RNase A should be stored at 4°C.
Kit Contents:
Catalog#
BMG-YD1281-01
BMG-YD1281-02
96-well DNA Plate
4x96
20x96
Lysate Clearance Plate
4x96
20x96
Buffer YPI
120 mL
600 mL
Buffer YPII
120 mL
600 mL
Buffer YPIII
150 mL
750 mL
Buffer SE
180 mL
900 mL
DNA Wash Buffer
3 x 50 mL
5 x 200 mL
Lyticase (units)
1500 U
7500 U
RNase A
400 µL
2000 µL
Elution Buffer
80 mL
400 mL
Materials required but not supplied:
Robotic station if using automated isolation.
Centrifuge with swing-bucket rotor (4,000 x g).
96-well 2.0 mL deep well plates.
Vacuum pump capable of achieving 300-400 mbar.
Standard vacuum manifold.
Oven or incubator preset to 70°C.
Before Starting:
Exam this handbook and get familiar with each step. Prepare all components and have the necessary materials ready.
Briefly spin down the RNase A vial and add the RNase A to Buffer YP I and store at 4°C.
DNA Wash Buffer: Add 200 mL (YD1291-01) or 800 mL (YD1291-02) 96-100% ethanol to each bottle before use.
Add 16 mL (YD1291-01) or 80 mL (YD1291-02) Buffer SE to Lyticase and dissolve before use.
Add 1% (v/v) of β-mercaptoethanol to Buffer SE before use.
