Ultrapure Water, Type 1, 500ml, Sterile
Product#: DCP-T1UH2O-500S
$32.31
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The Best Quality Control by Diagnocine Precision
Ultrapure Water, Type 1, 500ml, Sterile
Cat. No.: DCP-T1UH2O-500S| *pH option: | _Non pH Adjusted (pH 4.5 ~ pH 5 ) |
| _pH 7.4 |
CAS: 7732-18-5
Molecular Formula: H2O
Size: 500 ml
Feature:
- Sterile
- Free of DNAse, RNAse, Protease & Endonucleases
- Tested Electrolytic Conductivity Standard ASTM 0.05 μS/cm
- Deionized, 0.1uM filtered
- HPLC, GC, ICP-MS, Cell Culture, Chemistry & Molecular Studies
- For general PCR use only (i.e, DNA-based, gDNA and abundant source of RNA for the gene of interest)
- Not for delicate PCR use (i.e, endpoint or qPCR)
- RUO* | Research Purpose Only | Not for Human Consumption, Therapeutic & Clinical Use
- Shelf Life: 24 months
Description:
The purification process uses multiple steps of water purification methods, including demineralization, deionization, detoxification (i.e., endotoxin) and a relay of membrane filtration steps. The water purity is 0.015 to 0.005 microSiemens/cm, where the water does not conduct electricity and is near the purest form of water. The final sterilization steps include 2X (twice) sterilization with 0.1-micron filters (we can further purify by adding 0.04-micron if requested), reaching the cleanest and purest water in the market worldwide. No one in the market produces Type 1 Ultrapure water like DiagnoCine. All of our buffers use this water and purification methods as well.
The methods of DiagnoCine not only meet USP standards but far exceed requirements in terms of purity. The machines involved in Type 1 Ultrapure water are from the top major brands, with the best purification bead/filter units that guarantee the purity of water.
As the Ultrapure Type 1 water is exposed to the air, it rapidly picks up contaminants that affect its pH, and it has a low conductance. This causes instability in most pH meters, except for the pH meter that is specifically designed to work in ultra-pure water. Since the concentration of hydrogen ions in the water affects both pH and resistivity, the pH must lie within certain limits for a given resistivity reading. The pH of ultra-pure water can drop to 4.5 as it absorbs carbon dioxide from the atmosphere, but this does not mean that the water is contaminated. Simply picking up some ppm of CO2 from the air will cause the pH to fall.
* Researchers must understand that all Ultrapure water Type 1 in the market has low pH (not pH adjusted), and when using it, the final pH of the mixed solution may not be physiological pH 7.4. DiagnoCine provides NaOH pH-adjusted (pH 7.4) and non-pH-adjusted (pH 4.5-5) Ultrapure Type 1 water.
--- more info. ---
In a laboratory setting, the need for water purity, particularly Type I water, is critical for various scientific and analytical processes.
Type I water, also known as ultrapure water or deionized water, is the highest level of water purity achievable through water treatment processes.
Here are some reasons why Type I water is essential in a laboratory:
1. Precision and Accuracy:
Many laboratory experiments and analyses require precise and accurate measurements. Contaminants in water, even in trace amounts, can interfere with the accuracy of results. Type I water ensures that the water used in experiments does not introduce impurities that could skew the data.
2. Minimizing Contaminants:
Type I water is produced using multiple purification techniques, including deionization, reverse osmosis, and often additional treatments like UV or carbon filtration. These processes remove ions, organic compounds, bacteria, and other contaminants, reducing the potential for interference in experiments.
3. Reproducibility:
In scientific research, it is crucial to ensure that experiments can be reproduced by other researchers. Using Type I water with known and consistent purity levels helps achieve this reproducibility, as other scientists can use the same water source to replicate experiments.
4. Preventing Instrument Contamination:
In analytical chemistry and scientific instrumentation, particularly in techniques like HPLC (High-Performance Liquid Chromatography) and ICP-MS (Inductively Coupled Plasma Mass Spectrometry), even minute impurities in water can lead to instrument contamination, drift, or clogging. Type I water prevents these issues, ensuring the longevity and accuracy of expensive equipment.
5. Cell Culture and Biological Studies:
In cell culture and molecular biology experiments, the quality of water used can affect cell growth, viability, and experimental outcomes. Type I water is essential for maintaining cell cultures and conducting biological experiments free from contaminants.
6. Trace Element Analysis:
When analyzing trace elements or conducting trace metal analysis, any contamination from the water source can significantly impact results. Type I water ensures that the water itself does not contribute to trace element contamination.
7. Sensitive Techniques:
Some analytical techniques, such as atomic spectroscopy (e.g., ICP-OES or ICP-MS), require exceptionally pure water to avoid interference from background ions and to achieve the desired sensitivity.
8. Chemical Reagent Preparation:
In the preparation of chemical reagents, buffers, and standards, Type I water is used to ensure that the starting material is free of impurities that could alter the composition or properties of the solutions being prepared.
In summary, Type I water is necessary in a laboratory to maintain the highest level of water purity, which is essential for ensuring the accuracy, precision, and reliability of experimental results in various scientific disciplines. The specific requirements for water purity may vary depending on the nature of the experiments and analyses conducted in the laboratory, but in many cases, Type I water is the gold standard for water quality.
