AllView PAGE Buffer (*Only 1 Per Customer*)

Cat No.	Size	Link
FNK-DS520-500ML	500 ml
FNK-DS520-Trial	Trial

• Save cost and time: gradient gel is not needed!
• Only 13 minutes to complete electrophoresis by mini gel.
• Applicable to further assays: CBB staining, Western blot and etc...

1. Dilute the AllView PAGE Buffer 20 times with ultrapure water. (e.g. Add 25 ml of AllView PAGE Buffer to 475 ml of ultrapure water.)
2. Set the gel in electrophoresis chambers.
3. Fill the chambers with the 1 × AllView PAGE Buffer.
4. Load your samples and molecular weight markers.
5. Start electrophoresis.

• AllView PAGE Buffer is suitable for Laemmli gels (see below “Recommended usage”).
• If precast gels are used, the optimal acrylamide concentration may be different.
• AllView PAGE Buffer is not reusable.
• AllView PAGE Buffer is 20 × stock solution. Please dilute to 1 × solution before use.
• If precipitation of SDS is observed, completely dissolve it in a water bath (about 37°C) before use.
• Optimal electrophoretic time is depending on acrylamide concentration, gel size and voltage.

The recommended acrylamide concentration is 6% for resolving gel and 3% for stacking gel to separate the entire range of about 10 kDa to 250 kDa. Especially if the separation of low molecular weight proteins (10 kDa~30 kDa) is required, 10% resolving gel is recommended.
 

1. Gel preparation (Laemmli’s method)

 

	Stacking gel (6 ml)	Resolving gel (15 ml)
Gel percentage	3%	6%	10%
Ultrapure water	3.9 ml	8.05	6.05
1.5M Tris-HCl (pH8.8)	1.5	3.75	3.75
30% Acrylamide/Bis solution	0.6	3.0	5.0
10% SDS	0.06	0.15	0.15
TEMED	0.003 (3 µl)	0.00375 (3.75 µl)	0.00375(3.75 µl)
10% APS	0.06	0.05	0.05

Table 1. Recipes for polyacrylamide resolving and stacking gel (2 mini gels)

  

* DynaMarker® Protein MultiColor Stable II, Code#DM660

2. Electrophoretic image
The mobility of proteins using AllView PAGE Buffer is different from Laemmli electrophoresis running
buffer (Tris-Glycine-SDS). By using AllView PAGE Buffer and the Laemmli gels (Tris-HCl), a wide
range of protein sizes can be separated like a gradient gels.

 
Widely separated ! Only 13 min !

Fig. The mobility of proteins using AllView PAGE Buffer is different from Laemmli electrophoresis running buffer (Tris-Glycine-SDS). By using AllView PAGE Buffer and the Laemmli gels (Tris-HCl), a wide range of protein sizes can be separated like a gradient gels.
* DynaMarker® Protein MultiColor Stable II, Code#DM660