UltraClean PVSA-Sodium Phosphate, 1X

Cat No.: DCP-PHOSPVSA1X

Size: 500 mL and 1000 mL

Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.  

• World’s cleanest RNase-safe buffer for RNA handling, cell, and molecular biology experiments

PVSA-Sodium Phosphate, 1X is a ready-to-use, high-purity sodium phosphate buffer formulated to be RNase-safe for sensitive RNA and DNA workflows at near-neutral pH. It is supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, processing, and short-term storage. The buffer provides the well-defined buffering capacity of 1X sodium phosphate at physiological pH, making it suitable for use with purified nucleic acids, RNA-containing samples, and cells in downstream molecular biology applications where phosphate buffer is preferred.

Applications:

• RNA and DNA Resuspension: Resuspension and dilution of nucleic acids in a phosphate-based buffer with added RNase protection for storage or downstream analysis.
• RNA Handling and Short-Term Storage: Temporary storage of RNA-containing samples, lysates, or purified RNA in a controlled, near?neutral phosphate buffer.
• Sample Preparation for Enzymatic Assays: Handling and transfer of nucleic acids prior to downstream applications (e.g., RT, qPCR, library prep), as compatible with enzyme-specific phosphate tolerance.
• Cell and Tissue Processing: Gentle washing or processing of cells and tissues in workflows where RNA integrity is important and phosphate buffer is desired.
• General Molecular Biology: Any procedure requiring 1X sodium phosphate with enhanced RNA protection under defined pH and ionic conditions.

Advantages:

• RNase-Safe: PVSA provides robust inhibition of a broad spectrum of RNases, adding an extra layer of protection beyond standard nuclease-free practices.
• High Purity: Manufactured from molecular biology–grade sodium phosphate salts and PVSA, and finely filtered to minimize particulates, microbial contamination, and adventitious nucleases.
• Stable Near-Neutral pH: Phosphate buffering maintains a consistent, physiological-range pH that supports nucleic acid stability.
• Versatile Usage: Suitable for workflows that prefer phosphate over Tris or citrate, including some protein-adjacent and cell-based procedures.
• Ready-to-Use 1X Solution: Supplied at working strength to reduce preparation time, variability, and contamination risk.

Composition:

• Appearance: Colorless, clear solution; free of visible particulates.

• pH: 7.4

Storage and Shelf Life:

• Store at 2–8°C.

• Do not freeze.

• Shelf life: 12 months.

CUSTOMIZATION:  Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.

All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented.  The smallest size mycoplasma type can be about 0.2 microns.