UltraClean Krebs-Ringer Bicarbonate Buffer with Calcium Chloride, 10mM HEPES Buffer, and 0.2% Bovine Serum Albumin, without Glucose and Sodium Bicarbonate, 1X (Liquid)

FluxMPS™ UltraClean Krebs-Ringer Bicarbonate Buffer with Calcium Chloride, 10mM HEPES Buffer, and 0.2% Bovine Serum Albumin, without Glucose and Sodium Bicarbonate, 1X (Liquid)

An MPS-grade, 1X Krebs-Ringer Bicarbonate buffer formulated with calcium chloride, 10 mM HEPES buffer, and 0.2% bovine serum albumin, and deliberately prepared without glucose or sodium bicarbonate. Held at pH 7.4 and quadruple-stage filtered through 0.1 µm and 0.04 µm membranes, it is built for microchannel-safe use in tissue culture, primary adipocyte and lipolysis assays, islet perifusion, and pituitary bioassays.

• Quadruple-stage filtration: 0.1-micron membrane filtered TWICE and 0.04-micron membrane filtered TWICE
• Formulated to pH 7.4 at 1X concentration
• Supplemented with 10 mM HEPES buffer (pKa 7.3 at 37°C) for extended pH stability
• Contains 0.2% bovine serum albumin and calcium chloride
• Formulated without glucose and without sodium bicarbonate
• Base prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
• Sterility confirmed by 14-day incubation with no bacterial or fungal growth, per USP specification
• Custom pH, molarity, and additive formulations available on request

Engineered where standard buffers fail

Conventional single-pass 0.22 µm-filtered Krebs-Ringer buffers can carry subvisible particulates and unstable pH that accumulate in narrow microfluidic channels, drift outside a CO₂ incubator, and add background to sensitive metabolic assays. FluxMPS™ addresses each failure mode directly.

Quadruple-stage filtration system

This buffer is filtered through a 0.1-micron membrane TWICE and a 0.04-micron membrane TWICE, delivering microchannel-safe purity beyond what a single 0.22 µm pass can achieve.

1. 1

   0.1 µm Pre-filtration I

   Removes large particulates and aggregates, extending the life of downstream filters.

2. 2

   0.04 µm Pre-filtration II

   Retains fine particulates and bioburden, including organisms at the small end of the mycoplasma size range (approximately 0.2 µm).

3. 3

   0.1 µm Sterile-filtration I

   A second 0.1 µm pass provides redundant sterile-filtration assurance.

4. 4

   0.04 µm Sterile-filtration II — Final Polish

   A second 0.04 µm pass performs the final polish under ISO Class 5 (Class 100) aseptic fill conditions.

Where this buffer is used

This Krebs-Ringer Bicarbonate buffer is used for tissue cell culture, washing, preparation of primary adipocytes and lipolysis assays, pre-perifusion for islet viability and functionality studies, and pituitary bioassays. Its salt content supplies cells with water and inorganic ions, maintaining osmotic balance and physiological pH.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available for automated bioreactor and robotic liquid-handling platforms where valve and sensor protection is critical.

• Total Particulate Exclusion for closed-loop perfusion systems
• Valve & Sensor Protection in automated dispensing hardware
• Extended Perfusion Stability across long-duration runs

Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.

Specifications

Values below reflect this product's stated formulation and quality control parameters.

Full composition

Component concentrations are expressed in mg/L as released. This formulation excludes glucose and sodium bicarbonate.

Manufacturing & compliance

Manufactured under an ISO 13485-certified, CE-approved quality system, with final packaging, QA, and testing completed at the DiagnoCine R&D and Quality Testing Center.

How DCP-KRBB-GB1X compares

A comparison of this buffer's filtration architecture and formulation practices against conventionally filtered Krebs-Ringer buffers.

Frequently asked questions

Answers to common questions about this Krebs-Ringer Bicarbonate buffer.

Supporting literature

Curated literature relevant to Krebs-Ringer Bicarbonate buffers, HEPES buffering, and their laboratory applications.

1. Ringer S. A further contribution regarding the influence of the different constituents of the blood on the contraction of the heart. J Physiol. 1885. doi:10.1113/jphysiol.1885.sp000184
2. Krebs HA, Henseleit K. Untersuchungen uber die Harnstoffbildung im Tierkorper. Hoppe-Seylers Z Physiol Chem. 1932. doi:10.1515/bchm2.1932.210.1-2.33
3. Good NE, Winget GD, Winter W, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
4. Rodbell M. Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem. 1964. doi:10.1016/S0021-9258(18)91218-1
5. Lacy PE, Kostianovsky M. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes. 1967. doi:10.2337/diab.16.1.35
6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
7. Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. 2011. doi:10.1016/j.tcb.2011.09.005
8. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley-Blackwell. 2015. doi:10.1002/9781118873686
9. United States Pharmacopeia. USP <85> Bacterial Endotoxins Test. USP-NF. doi:10.31003/USPNF_M99035_02_01
10. United States Pharmacopeia. USP <788> Particulate Matter in Injections. USP-NF. doi:10.31003/USPNF_M99725_02_01