FluxMPS™ Click’s Medium (EHAA) Eagle Hanks’ Amino Acids w/o L-Glutamine, Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture

FluxMPS™ Click’s Medium (EHAA) Eagle Hanks’ Amino Acids w/o L-Glutamine, Sodium Bicarbonate, Mercaptoethanol

FluxMPS™ Click’s Medium (EHAA) is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Eagle Hanks’ Amino Acids formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic research. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Formulated as Eagle Hanks’ Amino Acids (EHAA) Click’s Medium, without L-Glutamine, Sodium Bicarbonate, or Mercaptoethanol for full researcher-controlled supplementation
• Low glucose (1.0 g/L) plus 275 mg/L Sodium Pyruvate and four nucleic acid precursors (Adenosine, Cytosine, Guanosine, Uridine) support immune-cell viability
• Hanks’ balanced salt buffering system: no CO₂ incubator required; add sodium bicarbonate only if a CO₂ atmosphere is used
• Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — Microfluidics Suitable
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
• Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ, USA
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon control
• Custom pH, glucose, salts, HEPES, and formulation modifications available on request

Engineered where standard media fails

Conventional 0.22 µm-filtered cell culture media can harbour mycoplasma (0.2–0.3 µm), subvisible particulates, and residual endotoxin that accumulate inside microfluidic channels as narrow as 10 µm — corrupting cytokine measurements, blocking microvalves, and generating false-positive biosensor signals. For immune-cell studies in MPS and OoC platforms, where mouse spleen cells, primary lymphocytes, or iPSC-derived immune models must respond authentically to defined stimuli, medium purity matters. FluxMPS™ is engineered to address these failure modes in a single formulation.^[1,2]

Quadruple-stage filtration system

FluxMPS™ Click’s Medium (EHAA) is processed through four sequential membrane filtration stages under ISO Class 5 (Class 100) aseptic conditions, reaching a 0.04 µm final pore size. ^[5]

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, aggregates, and cellular debris. Protects the first 0.04 µm final-filter cartridge from rapid fouling.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and provides mycoplasma-retentive filtration ahead of the second pair.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge under ISO Class 5 conditions.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter and aseptic fill under ISO Class 5 (Class 100) laminar-flow conditions, completing the microchannel-safe purity architecture.

Where FluxMPS™ Click’s Medium (EHAA) performs

Originally developed by Robert Click to study the in vitro immune response of mouse spleen cells using heterologous red blood cells (RBCs), Click’s Medium (EHAA) has evolved into a versatile platform for immune-cell biology, metabolic research, and microphysiological systems. The FluxMPS™ ultra-filtered formulation extends its utility to microfluidic organ-on-a-chip platforms.^[1,6]

Automated Bioreactors & Robotics

For automated bioreactor platforms and robotic liquid-handling systems where media contacts precision valves, sensors, and tubing over extended perfusion runs, an optional 0.01 µm (10 nm) MPS Grade variant of FluxMPS™ Click’s Medium (EHAA) is available on inquiry.

• Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulates that pass 0.04 µm membranes, protecting optical sensors and flow sensors from signal drift.
• Valve & Sensor Protection: Reduces micro-valve fouling and micro-electrode contamination risk in long-duration perfusion experiments.
• Extended Perfusion Stability: Supports media quality across multi-day automated perfusion cycles.

Inquiry Required: The 0.01 µm MPS Grade variant is produced in micro-batches on request. Contact support@diagnocine.com to discuss your automation platform requirements and lead time.

Analytical release specifications

Every batch of FluxMPS™ Click’s Medium (EHAA) is analytically characterised and released against the specifications below before shipping. Available pack sizes: 500 mL, 1000 mL. Certificate of Analysis (CoA) available on request at support@diagnocine.com.

Full composition (mg/L)

The complete formulation is reproduced below: 41 individually verified components across four categories — Inorganic Salts, Amino Acids, Vitamins, and Others. Nucleic acid precursors (Adenosine, Cytosine, Guanosine, Uridine) distinguish Click’s Medium (EHAA) from standard Hanks’ salt media. All mg/L values are as-formulated at 1X concentration. L-Glutamine, Sodium Bicarbonate, and Mercaptoethanol are omitted by design; each is supplied as a separate supplement per researcher protocol.

Manufacturing & compliance framework

FluxMPS™ Click’s Medium (EHAA) is produced under ISO 13485:2016 certified supplier facilities. All final packaging, quality assurance, and testing are performed at the Diagnocine R&D and Quality Testing Center. All customization and assembly is accomplished at Diagnocine Precision in Totowa, New Jersey, USA.

How DCP-CM-QB1X compares

FluxMPS™ Click’s Medium (EHAA) is processed to a 0.04 µm final filtration cut-off through a full quadruple-stage filtration architecture, verified by USP <788> particulate analysis and released under ISO 13485:2016 QMS testing.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Click’s Medium (EHAA) (DCP-CM-QB1X), its filtration system, supplementation requirements, and quality documentation.

Supporting literature

Curated peer-reviewed publications relevant to Click’s Medium (EHAA), immune-cell biology, organ-on-a-chip research, and microphysiological system media requirements. In-text citations (^[n]) throughout this page correspond to the entries below.
This product has been utilized by researchers at MD Anderson Cancer Center in scientific and laboratory applications.

1. Click RE, Benck L, Alter BJ. Immune responses in vitro. I. Culture conditions for antibody synthesis in vitro by normal mouse spleen cells. Cell Immunol. 1972;3(2):264–276. doi:10.1016/0008-8749(72)90071-0
2. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668. doi:10.1126/science.1188302
3. Ingber DE. Reverse engineering human pathophysiology with organs-on-chips. Cell. 2016;164(6):1105–1109. doi:10.1016/j.cell.2016.06.005
4. Bhattacharya A, Bhattacharya S. Mycoplasma contamination of cell cultures: Incidence, implications, and management. Indian J Med Microbiol. 2021;39(4):493–500. doi:10.1016/j.ijmmb.2021.07.007
5. United States Pharmacopeia. USP <788> Particulate Matter in Injections. USP-NF. Rockville, MD: USP; 2023. doi:10.31003/USPNF_M74950
6. Sung JH, Esch MB, Prot JM, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13(7):1201–1212. doi:10.1039/c3lc51138h
7. Rothbauer M, Zirath H, Ertl P. Recent advances in microfluidic technologies for cell-to-cell interaction studies. Lab Chip. 2018;18(2):249–270. doi:10.1039/c7lc00815e
8. Luni C, Giulitti S, Serena E, Ferrari L, Zambon A, Gagliano O, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13(5):446–452. doi:10.1038/nmeth.3832
9. van Midwoud PM, Janse A, Merema MT, Groothuis GM, Verpoorte E. Comparison of biocompatibility and adsorption properties of different plastics for advanced microfluidic cell and tissue culture models. Anal Chem. 2012;84(9):3938–3944. doi:10.1021/ac300771z
10. Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol. 2007;8(10):839–845. doi:10.1038/nrm2236