ReverTra Ace® qPCR RT Master Mix, Trial (Only 1 Per Customer)
ReverTra Ace® qPCR RT Master Mix (*Only 1 Per Customer*)
A 5× premix reverse-transcription master mix for real-time PCR — high-efficiency cDNA synthesis from RNA in a simple 15-minute protocol.
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Only 1 Per Customer
Ordering
| Product Name | Cat No. | Storage | Order |
|---|---|---|---|
| ReverTra Ace® qPCR RT Master Mix, Trial | TYB-FSQ-201S | Store at -20 °C | Current page |
| ReverTra Ace® qPCR RT Master Mix, 200 rxn | TYB-FSQ-201 | Store at -20 °C | Order |
Description
The ReverTra Ace® qPCR RT Kit efficiently synthesizes high quality cDNAs from RNA templates for real-time PCR. This kit contains the reverse transcriptase "ReverTra Ace®" and a RT buffer optimized for the synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in just 15 minutes. ReverTra Ace® is a mutant-type M-MLV reverse transcriptase that is one of the best of its class in the market today.
The protocol is simple, and the reaction can be completed in 15 min.
ReverTra Ace® is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
ReverTra Ace® qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace® qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase "ReverTra Ace®" , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.
Features
The optimized RT buffer and primer mix enable highly efficient reverse transcription.
The reaction can be completed in 15 minutes. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (patent pending).
Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.
Specifications
- Robust M-MLV RT enzyme
- 10 µl reaction volume per sample
- 5x RT Buffer
Applications
cDNA synthesis for real-time PCR
Components
- 5x RT Buffer 400 µl
- Enzyme Mix 100 µl
- Primer Mix 100 µl
- Nuclease-free water 1000 µl × 2
Application Data
Example 1. Comparison of cDNA yields using various reverse transcription kits
cDNA was synthesized from human cell line (HeLa cell) total RNA with the ReverTra Ace® qPCR RT kit. Subsequently, quantification of β-actin and polymerase ε mRNA were performed by real-time PCR with 100 ng cDNA, in conjunction with the SYBR® Green Realtime PCR Master Mix [Code No. QPK-201].
Example 2. Detection of a rare expressing gene
Several 1-µl cDNA aliquots were prepared from 100 ng human cell line total RNA in a volume of 20 µl using various reverse transcription kits. Subsequently, a low expressing gene (TNF-α) was detected with real-time PCR kit (SYBR® Green Realtime PCR Master Mix [Code No. QPK-201].
The Ct values and success rates of the ReverTra Ace qPCR RT kit were greater than those from the other commercial kits.
Publications
Powered by Bioz — literature citing this productFrequently Asked Questions
A: This step can be done up to 1 hr. Its real purpose is to extend the construction of cDNA for genes that are rich with secondary structure on the 3' end, especially for cases where the oligo?dT is used and you are starting off from the 3' end. It is also useful for experiments where your gene of interest may have a very long 3'UTR region with more regulatory secondary structure on the 3'UTR region.
It does not hurt to leave it up to 1 hr (typically up to 30 min) if one wishes to maximize the extension of all genes. However, if you know that your gene amplifies without problems and the PCR amplicon is clearly visual after the typical protocol, you do not need to do this long extension.
