Titansphere TiO 5um
Cat No: FNK-502075000
Size: 500 mg
Storage: RT
Storage: RT
Applications
Efficient Purification from HeLa Cell Lysate The data at right shows the superior performance of Titansphere TiO using the HeLa Cell Lysate consisting mainly of non-phosphorylated peptides.
Titansphere TiO shows exceptional selectivity - almost 90% of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. A competitive TiO product is shown, binding mainly non-phosphorylated peptides and a much lower total number of discreet phosphopeptide species.
Sample: HeLa Cell Lysate, Sample volume: 50 μg,
Titansphere TiO volume: 1 mg
Titansphere TiO shows exceptional selectivity - almost 90% of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. A competitive TiO product is shown, binding mainly non-phosphorylated peptides and a much lower total number of discreet phosphopeptide species.
Sample: HeLa Cell Lysate, Sample volume: 50 μg,
Titansphere TiO volume: 1 mg
Compare Titansphere TiO with IMAC
The graph at right shows how Titansphere TiO compares to an IMAC enrichment using Arabidopsis cell extract. Titansphere TiO provides substantially higher total capacity and a much higher number of discreet phosphopeptides isolated.
Sample: Arabidopsis Cell Extract, Sample volume: 100 μg,
Titansphere TiO volume: 1 mg
Sample: Arabidopsis Cell Extract, Sample volume: 100 μg,
Titansphere TiO volume: 1 mg

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