Efficient Purification from HeLa Cell Lysate The data at right shows the superior performance of Titansphere TiO using the HeLa Cell Lysate consisting mainly of non-phosphorylated peptides.
Titansphere TiO shows exceptional selectivity - almost 90% of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. A competitive TiO product is shown, binding mainly non-phosphorylated peptides and a much lower total number of discreet phosphopeptide species.
Sample: HeLa Cell Lysate, Sample volume: 50 μg,
Titansphere TiO volume: 1 mg
Compare Titansphere TiO with IMAC
The graph at right shows how Titansphere TiO compares to an IMAC enrichment using Arabidopsis cell extract. Titansphere TiO provides substantially higher total capacity and a much higher number of discreet phosphopeptides isolated.