Taq DNA Polymerase Hot-Start (+dNTPs) with Robust Buffer
Taq DNA Polymerase Hot-Start (+dNTPs) with Robust Buffer
Cat.No: FNK-02-005Size: 200 U
Storage: -20℃
Concentraction: 1 U/µl
Purity: > 95% as examined by SDS-PAGE. End- and Exo-DNase free
PCR Test: Good PCR amplification has been confirmed with Lambda phage DNA as template.
Product Description
Product is a mixture of Taq DNA polymerase and anti-Taq DNA polymerase antibody (monoclonal) with neutralizing activity of the enzyme. Until the start of the reaction, the antibody binds to Taq DNA polymerases to inhibit the production of nonspecific products. After the reaction starts, when the temperature becomes high, the antibody is deactivated in a short time, and PCR reaction with high specificity is started. Efficient amplification of specific PCR reaction products can be achieved, especially by suppressing non-specific reactions before the cycle or early in the cycle.
*Hot-Start reaction system is suitable for PCR reaction enabling efficient and specific DNA amplification with a variety of primers.
Definition of Activity
One unit is defined as the amount of enzyme that can incorporate 10nmols of total dNTPs into an acid-insoluble material in 30 minutes at 74℃ when activated salmon sperm DNA was used as template / primer.
Components
Taq DNA polymerse Hot-Start Mixture: Taq DNA polymerase (1 unit/µl), 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% Tween20, 0.5% Igepal CA630, anti Taq antibody (0.8 μg/ml) (02-Atq) (02-Hta 200µl)
10 x Robust Buffer (Taq):（02-Trb 1.0 ml）
2.5 mM (each) dNTPs:（02-Dnth 800 µl）
Application
Please Note: All products are FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC PROCEDURES. NOT FOR MILITARY USE.
Related Products
| SKU# | Description | Link |
|---|---|---|
| FNK-02-004 | Taq DNA Polymerase Hot-start(+dNTP) | GO! |
| FNK-02-004-5 | Taq DNA Polymerase Hot-start(+dNTP) (5x200U) | GO! |
| FNK-02-005-5 | Taq DNA Polymerase Hot-Start (+dNTPs) with Robust Buffer (5x200U) | GO! |