SDS Stripping Buffer

Product Overview

ISO 13485 Certified Manufacturing

FluxMPS™ SDS Stripping Buffer

FluxMPS™ SDS Stripping Buffer is a harsh-strength Western blot stripping reagent formulated with 0.5 M Tris-HCl, 10% SDS, and 50 mM TCEP to aggressively dissociate high-affinity antibodies from immunoblot membranes. Sterile-filtered through DiagnoCine's quadruple-stage 0.1 µm (twice) and 0.04 µm (twice) membrane architecture, it delivers a clean, DNase/RNase-free reagent for reliable membrane reprobing. Manufactured under ISO 13485-certified, CE-approved processes for research applications requiring dependable, reproducible antibody stripping.

Quadruple-stage sterile filtration: 0.1-micron membrane twice and 0.04-micron membrane twice
Harsh stripping formulation: 0.5 M Tris-HCl, 10% SDS, 50 mM TCEP
TCEP reducing agent — more effective than DTT or beta-mercaptoethanol for disulfide bond cleavage
pH 6.8, clear, colorless liquid; DNase- and RNase-free
Designed for high-affinity antibody removal and reliable membrane reprobing in Western blot workflows
Manufactured in ISO 13485-certified, CE-approved facilities
Customizable to MILD or MEDIUM stripping formulations, pH, and additive content on request

SKU: DCP-SDSSB1X UNSPSC 12161703 Other Buffers — Stripping Protein

SDS Stripping Buffer (Harsh), 500 mL

FormulationTris-HCl / SDS / TCEP
pH6.8
Tris-HCl0.5 M
SDS Concentration10%
TCEP Concentration50 mM
AppearanceClear, Colorless Liquid
Sterility0.1 µm x2 + 0.04 µm x2
DNase / RNaseNone Detected
Storage4°C or Room Temperature
Shelf Life1 Year

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard stripping buffers fall short

Conventional 0.22 µm-filtered stripping buffers can carry subvisible particulates and bioburden into sensitive membrane reprobing workflows, contributing to variable background and inconsistent antibody removal. FluxMPS™ SDS Stripping Buffer is built around a defined formulation and a multi-stage sterile filtration process to deliver consistent, reproducible stripping performance.

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Microchannel-safe purity

Sequential 0.1 µm (x2) and 0.04 µm (x2) filtration removes fine particulates well beyond a single-pass 0.22 µm filter, consistent with USP <788> particulate-control principles, protecting membrane integrity in downstream immunoassay and chip-based workflows.

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Precise, stable pH

Formulated at pH 6.8 with a defined 0.5 M Tris-HCl buffering system, giving consistent dissociation conditions for high-affinity antibody-antigen complexes from lot to lot.

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Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) to minimize trace contaminant interference during BET-aligned quality workflows.

visibility

Low background for imaging & assays

Verified DNase- and RNase-free after 18-hour incubation studies, helping minimize background signal in downstream immunodetection and imaging steps.

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Defined, traceable composition

Every lot is characterized for Tris-HCl, SDS, and TCEP concentration, appearance, and pH, delivering traceable, reproducible stripping performance.

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Customization on demand

pH, salt content, and additive load can be adjusted, including MILD and MEDIUM stripping buffer formulations with low/high pH and high salt content — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ SDS Stripping Buffer is sterile-filtered through a quadruple-stage architecture — the 0.1-micron membrane applied twice and the 0.04-micron membrane applied twice — delivering an ultra-clean, low-particulate stripping reagent for Western blot and membrane-based immunoassay workflows.

1

0.1 µm Pre-filtration I

Large particulate and aggregate removal, extending the service life of downstream filtration stages.
2

0.04 µm Pre-filtration II

Retention of fine particulates and bioburden ahead of final sterile filtration.
3

0.1 µm Sterile-filtration I

Second-pass 0.1 µm filtration adds redundancy to the sterile filtration process.
4

0.04 µm Sterile-filtration II — Final Polish

Final 0.04 µm polish into an ISO Class 5 (Class 100) aseptic fill environment. This pore size is smaller than the smallest known mycoplasma organisms, approximately 0.2 micron, helping prevent mycoplasma contamination in the finished buffer.

Performance vs. conventional buffer

Sequential 0.1-micron (x2) and 0.04-micron (x2) filtration captures particulates and bioburden that a single 0.22-micron pass allows through, supporting a cleaner, more consistent stripping reagent for sensitive membrane reprobing work.

0.04 µm

Final filtration stage

4

Total filtration stages

Sterility is maintained through filtration into a controlled, ISO Class 5 (Class 100) aseptic fill environment, consistent with USP <71> sterility principles for aqueous laboratory buffers.

FluxMPS SDS Stripping Buffer DCP-SDSSB1X quadruple-stage 0.1 micron and 0.04 micron filtration diagram for Western blot and organ-on-a-chip immunoassay applications | Diagnocine — **Figure 1.** Quadruple-stage filtration architecture (0.1-micron membrane x2, 0.04-micron membrane x2) used to sterile-filter FluxMPS™ SDS Stripping Buffer.
© Diagnocine® — DCP-SDSSB1X

Applications

Where FluxMPS™ SDS Stripping Buffer fits your workflow

This harsh-strength stripping buffer is intended for removing high-affinity antibodies from Western blot membranes and immobilized immunocomplexes, enabling clean re-probing across a range of protein detection and membrane-based immunoassay workflows.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For laboratories integrating membrane stripping and reprobing into automated liquid-handling or robotic Western blot workstations, an optional 0.01-micron (10 nm) ultra-filtered variant of this buffer is available on request to further reduce particulate load in automated fluidic pathways.

Total Particulate Exclusion for automated fluidic pathways
Valve & Sensor Protection in robotic liquid-handling systems
Extended Perfusion Stability for continuous or repeated-cycle instrumentation

Inquiry Required: The 0.01-micron (10 nm) ultra-filtered grade is produced to order. Contact support@diagnocine.com to request this variant for your automated platform.

Microfluidics

Micro Physiological System (MPS) & Chip

Supports membrane stripping and reprobing steps for miniaturized, chip-based Western blot and immunoassay platforms.

OoCToCBoCLoCMPS

Sample Preparation

Wash, Dilution & Reconstitution

Used within membrane wash and stripping steps of broader sample preparation and reprobing workflows.

LysisDilutionReconstitutionRinse

Stem Cell Biology

iPSC-Derived Model Handling

Enables stripping and reprobing of membranes probed with lysates from iPSC-derived cell models.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cell Perfusion

Supports reprobing of membranes from endothelial and primary cell lysates for multiplexed target detection.

HUVECsHAECsPrimary hepatocytes

Immunoassays

ELISA, Blotting & Blocking

Primary use case: harsh stripping of high-affinity antibodies for Western blot membrane reprobing.

ELISAWestern blotIHCIF

Live-Cell Imaging

Microscopy & Optical Sensing

After stripping, membranes can be reprobed for targets correlated with confocal and biosensor-based detection.

ConfocalBiosensorsTEER

Technical Specifications

Specifications at a glance

Every parameter below reflects data stated for this product; where a parameter is not characterized for this buffer, it is omitted rather than approximated.

Physical & Chemical Parameters

Parameter	Specification
Formulation / Composition	Tris-HCl, SDS, TCEP buffer system
Appearance	Clear, Colorless Liquid
pH USP <791>	6.8
Tris-HCl Concentration	0.5 M
SDS Concentration	10%
TCEP Concentration	50 mM

Sterility, Purity & Safety Parameters

Parameter	Specification
Sterility USP <71>	Filtered 0.1-micron membrane twice and 0.04-micron membrane twice in a sterile environment
DNase Activity	None detected after 18 hr incubation with plasmid DNA at room temperature
RNase Activity	None detected after 18 hr incubation with ribosomal RNA at room temperature
Water Quality	Ultrapure Type 1 water (18.2 MΩ·cm)
Manufacturing Standard ISO 13485	ISO 13485-certified, CE-approved facility
Fill Environment	ISO Class 5 (Class 100) aseptic fill

Storage, Handling & Logistics

Parameter	Specification
Storage Temperature	4°C (long-term storage; SDS may precipitate — redissolve at room temperature) or room temperature
Shelf Life	1 year

Raw Materials & Regulatory Traceability

Parameter	Specification
Manufacturing Facility	ISO 13485-certified and CE-approved facility (DiagnoCine Precision supplier network)
Final QA & Packaging	Completed at the DiagnoCine R&D and Quality Testing Center
Customization & Assembly	Performed at DiagnoCine Precision, Totowa, New Jersey, USA
Intended Use	Research Use Only (RUO)

Formulation

Full composition

FluxMPS™ SDS Stripping Buffer is a defined Tris-HCl / SDS / TCEP formulation, with every component and concentration released on a per-lot basis.

Component	CAS Number	Concentration
Tris-HCl	1185-53-1	0.5 M
SDS (Sodium Dodecyl Sulfate)	151-21-3	10%
TCEP (Tris(2-carboxyethyl)phosphine)	51805-45-9	50 mM

CUSTOMIZATION: Please inquire if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed. MILD or MEDIUM stripping buffers with low/high pH and high salt can be customized. Contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

FluxMPS™ SDS Stripping Buffer is produced under a controlled quality system, with final release testing and customization completed at DiagnoCine's own facilities.

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ISO 13485:2016 QMS

Manufactured under ISO 13485-certified and CE-approved facilities.

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Ultrapure Type 1 Water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for cleanroom-level purity.

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ISO Class 5 Fill & Finish

Quadruple-stage 0.1-micron (x2) / 0.04-micron (x2) filtration culminating in a sterile, ISO Class 5 fill.

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Micro-Batch Precision

Final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization and assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA.

Endotoxin Testing USP <85> BET

Endotoxin testing follows USP <85> Bacterial Endotoxins Test methodology as part of DiagnoCine's sterile buffer release framework; contact support@diagnocine.com for lot-specific results.

Particulate Control USP <788> Method 2

Particulate matter is controlled through the product's quadruple-stage 0.1-micron / 0.04-micron filtration architecture, in line with USP <788> Method 2 principles.

Osmolality USP <785>

Osmolality testing is applied across DiagnoCine's aqueous buffer platform as applicable; this product is characterized primarily by pH and reducing/denaturing agent concentration relevant to its stripping application.

Documentation / CoA

A lot-specific Certificate of Analysis documenting appearance, pH, sterility/filtration, DNase, and RNase testing is available on request.

A Certificate of Analysis (CoA) for DCP-SDSSB1X is available on request — contact support@diagnocine.com.

Product Comparison

How DCP-SDSSB1X compares

A structural comparison against conventional, single-pass 0.22-micron-filtered stripping buffer.

Parameter	DCP-SDSSB1X (FluxMPS™)	Conventional Buffer (0.22 µm filtered)	Standard Alternative (0.22 µm filtered)
Defined high-strength SDS/TCEP formulation	check_circle	cancel	cancel
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4	1	1
TCEP-based reducing agent (vs. DTT/BME)	check_circle	cancel	cancel
Verified DNase/RNase-free	check_circle	cancel	cancel
Manufacturing QMS (ISO 13485)	check_circle	cancel	cancel
Membrane-based / chip-integrated immunoassay compatibility	check_circle	cancel	cancel
Custom formulation (pH, salts, additives)	check_circle	cancel	cancel

FAQ

Frequently asked questions

Answers to common questions about DCP-SDSSB1X SDS Stripping Buffer.

DCP-SDSSB1X is primarily designed for high-affinity antibody stripping and reprobing of Western blot membranes. Where membrane-based immunoassays are integrated into microfluidic or chip-based workflows, its quadruple-stage filtration and sterile format support use within those environments.

DCP-SDSSB1X is filtered through a quadruple-stage architecture — the 0.1-micron membrane twice and the 0.04-micron membrane twice. This sequential filtration removes finer particulates than a single 0.22-micron pass allows through, supporting a cleaner buffer for sensitive membrane reprobing work.

The buffer is supplied at pH 6.8 with a 0.5 M Tris-HCl buffer system, 10% SDS, and 50 mM TCEP. Other concentrations, pH values, and additions of chemicals, compounds, proteins, or supplements can be customized on request, including MILD or MEDIUM stripping buffer formulations with low/high pH and high salt content.

The pH of 6.8 is measured on the buffer as supplied; a specific measurement temperature is not stated for this parameter. The buffer is stable when stored at 4°C for long-term storage (SDS may precipitate at this temperature but redissolves upon returning to room temperature without affecting performance) or at room temperature, for a shelf life of 1 year.

Yes. DiagnoCine can customize concentrations and add chemicals, compounds, proteins, or supplements as needed, and can also formulate MILD or MEDIUM stripping buffers with low/high pH and high salt content. Contact support@diagnocine.com to discuss your protocol.

An endotoxin specification is not part of this product's standard release data. If a defined endotoxin limit is required for your application, contact support@diagnocine.com to discuss custom testing.

Yes. A lot-specific CoA is available on request, documenting appearance, pH, sterility/filtration (0.1-micron x2, 0.04-micron x2), DNase activity, and RNase activity testing. Contact support@diagnocine.com to request a copy.

Scientific References

Supporting literature

No product-specific citations were provided for DCP-SDSSB1X; the curated literature below supports the underlying chemistry and quality principles applied to this buffer.

Kaufmann SH, Ewing CM, Shaper JH. The erasable Western blot. Anal Biochem. 1987. doi:10.1016/0003-2697(87)90496-8
Yeung YG, Stanley ER. A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing. Anal Biochem. 2009. doi:10.1016/j.ab.2009.03.017
Burnette WN. Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose. Anal Biochem. 1981. doi:10.1016/0003-2697(81)90281-5
Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994. doi:10.1006/abio.1994.1173
Getz EB, Xiao M, Chakrabarty T, Cooke R, Selvin PR. A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry. Anal Biochem. 1999. doi:10.1006/abio.1999.4302
Uphoff CC, Drexler HG. Detecting mycoplasma contamination in cell cultures by polymerase chain reaction. Methods Mol Biol. 2011. doi:10.1007/978-1-61779-108-6_9
Rottem S, Barile MF. Beware of mycoplasmas. Trends Biotechnol. 1993. doi:10.1016/0167-7799(93)90121-Z
Bass JJ, Wilkinson DJ, Rankin D, et al. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017. doi:10.1111/sms.12702
Junqueira-Neto S, Batista IA, Costa JL, Melo SA. Bioanalytical methods for membrane protein detection and reprobing in microfluidic immunoassay platforms. Micromachines. 2018. doi:10.3390/mi9080387