Rad52 Human, Recombinant (100µg)

Rad52 Human, Recombinant

DiagnoCine offers excellent Rad51 I Antibodies and Recombinant proteins  to researchers studying DNA repair, Mitochondrial DNA synthesis, Replication Stress,  Homologous Recombination, and Meiosis.

Human diseases include multiple cancer types  (breast cancer, prostate cancer, renal cancer, and lung cancer & others), aging, congenital mirror movement disorder, and Fanconi anemia.

Rad51 I Antibodies have excellent quality and this highly pure antibody can be adapted for Western blotting, Immunoprecipitation, and Immunofluorescent staining, and other research protocols with optimization.

Cat. No. FNK-10-004
Store at -80°C
Size- 100µg
Purity＞90%(SDS-PAGE)，
Host E. coli，
Concentration1.0 mg/ml

Description 

Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands (1).

Rad52 functionally and physically interacts with Rad51, Dmc1, and RPA in recombination processes. In radiation damaged cells, Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci (2).

The product was highly purified from E. coli over-expressing human Rad52 protein as a recombinant protein (3).

Applications

1) Studies on homologous recombination in mammals including human
2) Studies on the interaction of Rad51 protein with various proteins
3) To be used as a standard for Western blotting 

Specification

Purity > 90% as judged from SDS-PAGE analysis (Fig.1)
Form 1.0 mg/ml in 20 mM potassium phosphate pH 7.5, 200 mM KCl, 2 mM 2ME, 0.5 mM EDTA, 20% glycerol
Storage -70℃
Data Link UniProtKB/Swiss-Prot P43351 (RAD52_HUMAN) 

References 

1. Friedberg EC et al DNA Repair and Mutagenesis 2nd ed., ASM Press (2006)
2. Kitao H & Yuan Z M “Regulation of ionizing radiation-induced Rad52 nuclear foci formation by c-Abl-mediated phosphorylation.” J Biol Chem 277:48944-48948 (2002) PMID: 12379650
3. Kagawa W et al ”Homologous pairing promoted by the human Rad52 protein.” J Biol Chem 276: 35201-35208 (2001) PMID: 11454867

* This product was used in reference 3.