Heparin-GNP

Product#: GC-HEP-500
$673.00
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Ships in 1-2 weeks

Heparin-GNP

Cat. No. GC-HEP-500
Store at 4°C
Size 500µl


Description 

SGNP(Sugar chain - immobilized Gold Nano-Particle) is a gold nano particles immobilized with structurally defined sugar chains, showing red-purple color (λmax = ca. 530 nm). SGNP is a very convenient tool for evaluating sugar chain – protein interaction, since the interaction can be detected visually, and can be quantified from the change of OD at 530 nm. There are several applications as follows:

  1. Aggregation of protein
  2. Inhibition assay
  3. Dot-blotting
  4. Isolation and purification of lectin from a crude extract
Application#1: Aggregation assay of protein(s)
Regularly sugar chain binding proteins, such as lectins, possess multiple binding sites for sugar chains. By the addition of SGNP to the protein solution, the protein may form aggregates with sugar chains immobilized SGNP in a short time of period.
The change can be seen visually, or can be quantified by measuring OD at around 530 nm. Using SGNP, the binding properties (selectivity, dissociation constant (KD), specificity, etc.) are easily evaluated.
Example
G-00M-100._Example1jpg.jpg

Application#2: Inhibition assay
The sugar chain binding potency of the trace amount of your samples can be detected. 
* Independent on the valency of sugar-binding proteins.
G-00M-100_Example3.jpg
 
Application#3: Isolation and identification of target protein from a crude extract
Quick isolation and identification of protein(s) having multi-binding sites (lectin) from a plant-derived crude extract of cell-lysate are available using SGNP. Once you get an aggregate by mixing appropriate SGNP with your extract or lysate, the aggregate can be directly applied for SDS-PAGE. Then, the protein band(s) in SDS-gel is analyzed according to the proteomics procedure.
 
G-00M-100_Example4.jpg
SGNP (Sugar chain immobilized Gold Nano Particle)
SGNPs
G-00M-100_fig1.jpg

SGNP set 
G-00M-100_fig2.jpg 
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