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FluxMPS™ RPMI 1640 Medium w/o Glutamine: 1X Liquid

Product#: DCP-RPMI-Q1X
$34.10
DCP-RPMI-Q1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o Glutamine: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid RPMI 1640 medium formulated without L-glutamine for fresh, on-demand nitrogen addition. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red | [-] L-Glutamine.

  • High-glucose formulation (2.0 g/L D-Glucose) with sodium pyruvate (110 mg/L) providing a defined, adjustable carbon source for lymphocyte and hematopoietic energy metabolism
  • Glutathione (reduced), 1.0 mg/L, included for redox and antioxidant support in redox-sensitive immune cell types
  • L-Glutamine omitted so it can be added fresh at time of use, avoiding storage-related ammonia accumulation during extended lymphocyte culture
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, well below the 0.2–0.3 µm mycoplasma size range
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
  • Phenol red (5.3 mg/L) and sodium bicarbonate (2.0 g/L) retained for visual pH monitoring and 5% CO₂ buffering
  • Custom pH, nutrient, and salt modifications available on request — contact support@diagnocine.com
CAT. NO.
DCP-RPMI-Q1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 w/o Glutamine — 1X Liquid
  • Media familyRPMI 1640
  • Glucose2000 mg/L (2.0 g/L)
  • Formulation[+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine
  • AppearanceRed-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280 - 320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized particles, subvisible particulates, and manufacturing residues that can confound sensitive immune cell assays and clog microfluidic channels. FluxMPS™ addresses these gaps through a validated, redundant filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance delivers particulate-controlled media for suspension immune cell OoC and flow cytometry workflows.

target

Metabolic support for lymphocytes

2.0 g/L D-glucose and 110 mg/L sodium pyruvate provide a defined, adjustable carbon source for T cell, B cell, and NK cell energy metabolism studies.

water_drop

Ultrapure-grade water

Processed with Ultrapure Type 1 water (18.2 MΩ·cm) under controlled trace-metal and organic-carbon (TOC) limits, supporting consistent lot-to-lot performance.

visibility

Low background for imaging

0.04 µm filtration reduces particulate baseline for confocal microscopy and biosensor-integrated OoC platforms. This formulation is phenol red–containing; a phenol red–free custom variant is available for fluorescence-sensitive assays.

science

Rich, stable nutrient profile

40 verified components across amino acids, vitamins, inorganic salts, and glutathione (reduced) support demanding lymphocyte and hematopoietic cell requirements, delivered particulate-controlled for OoC use.

tune

Customization on demand

pH, glucose concentration, salts, HEPES buffering, and nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates not captured by a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final pore size — below the 0.2–0.3 µm mycoplasma size range
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMI-Q1X RPMI 1640 w/o Glutamine Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) Microfluidics Suitable cell culture media for organ-on-a-chip and OoC applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-Q1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-Q1X — RPMI 1640 w/o Glutamine — delivers 0.04 µm filtered, Microfluidics Suitable purity for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant — the six-stage ultra nano-filtered line beyond this Microfluidics Suitable 0.04 µm product — is available on request for automated bioreactor and robotic perfusion systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration delivers particulate-controlled media for these sensitive cultures.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where particulate-controlled media supports reliable microchannel performance.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Endotoxin release specification < 0.05 EU/mL supports CAR-T manufacturing and TIL expansion protocols where endotoxin control is a process requirement.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Bicarbonate-buffered, phenol red–containing formulation supports general metabolic flux and ¹³C-tracing workflows in suspension format. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium.

¹³C tracingGlycolysisFlux analysis
Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate baseline supports flow cytometry and confocal imaging of immune cells; a phenol red–free custom variant is available for fluorescence-sensitive channels.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine
Appearance Red-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
pH USP <791> 7.4
Osmolality USP <785> 280 - 320 mOsm/kg H2O
Total ingredients 40 components across 4 categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ required (sodium bicarbonate buffering)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 w/o Glutamine: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains glutathione (reduced) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.

INORGANIC SALTS
Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2000.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
AMINO ACIDS
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
VITAMINS AND OTHERS
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
Phenol red sodium salt 34487-61-1 5.300
Sodium Pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMI-Q1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm water processed under controlled trace-metal and organic-carbon (TOC) limits.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, controlled per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280 - 320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability, lot number, and expiry available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMI-Q1X compares

FluxMPS™ DCP-RPMI-Q1X vs. conventional 0.22 µm–filtered RPMI 1640 and published supplier specifications.

Parameter DCP-RPMI-Q1X (FluxMPS™) Comparison / Alternative Products
Grade Microfluidics Suitable Not specified (conventional media typically not tier-graded)
Formulation RPMI 1640 w/o L-Glutamine; 2.0 g/L glucose, sodium pyruvate, sodium bicarbonate Varies by supplier; L-glutamine commonly pre-added
Final filtration pore size 0.04 µm 0.22 µm (typical)
Number of filtration stages 4 (Quadruple-stage: 0.1 µm ×2 + 0.04 µm ×2) 1 (single-stage, typical)
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel Not typically applied
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate testing check_circle Yes (Method 1) cancel Not typically published
Water quality Ultrapure Type 1, 18.2 MΩ·cm Purified water (typical)
Manufacturing QMS ISO 13485:2016 ISO 9001 or unspecified
Microfluidic channel compatibility check_circle Yes cancel Risk of channel clogging
Custom formulation check_circle Yes — support@diagnocine.com cancel Not typically offered

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-Q1X — RPMI 1640 w/o Glutamine.

Yes. DCP-RPMI-Q1X is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size. This Microfluidics Suitable RPMI 1640 formulation is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models where particulate carryover from standard 0.22 µm–filtered media can interfere with microchannel geometry and cell signaling readouts.
FluxMPS™ uses four sequential filters arranged as two dedicated prefilter/final-filter pairs: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — Polish. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter, giving full redundancy across the train and a validated 0.04 µm final pore size, well below the 0.2–0.3 µm mycoplasma size range.
L-glutamine is omitted so it can be added fresh at the time of use (commonly 2 mM for RPMI-based cultures), avoiding the spontaneous degradation to ammonia that liquid glutamine undergoes in storage. Ammonia accumulation can suppress lymphocyte proliferation and alter cytokine profiles in extended T cell and B cell cultures. Add L-glutamine, or a stabilized dipeptide substitute such as L-alanyl-L-glutamine, directly to the medium before use.
Yes. This formulation is buffered with 2.0 g/L sodium bicarbonate and requires a 5% CO₂ incubator atmosphere to maintain pH near 7.4. Phenol red (5.3 mg/L) is included as a visual pH indicator.
Yes. Fetal bovine serum, defined serum-free supplements, growth factors, and antibiotics can be added as required. Because serum and protein-containing supplements contain lipoproteins and immunoglobulins larger than the medium's own 0.04 µm final filter cut-off, filter serum-containing additions separately through a 0.2 µm low-protein-binding PES or PVDF membrane rather than through the base medium's filtration train. Contact support@diagnocine.com for custom co-formulation.
DCP-RPMI-Q1X is released to a specification of less than 0.05 EU/mL, determined by LAL assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before it ships.
Yes. A full CoA is available per lot, covering appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (LAL, USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), raw-material traceability, lot number, and expiry. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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