FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
FluxMPS™ DCP-RPMI-QP1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid RPMI 1640 formulation engineered for lymphocyte, T cell, and hematopoietic cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile filtration. L-Glutamine and sodium pyruvate are omitted so metabolic nitrogen and carbon inputs can be set independently at time of use.
- Formulation retains 2.0 g/L D-glucose and 2.0 g/L sodium bicarbonate; L-glutamine and sodium pyruvate omitted for independent, fresh supplementation
- Reduced glutathione (1.0 mg/L) included — antioxidant support for redox-sensitive lymphocyte and hematopoietic cultures
- Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- pH 7.4, osmolality 280–320 mOsm/kg H2O, buffered with sodium bicarbonate for a standard 5% CO2 atmosphere
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Custom pH, nutrient, and salt modifications available on request
- Media familyRPMI 1640
- Glucose2000 mg/L (2.0 g/L)
- L-GlutamineNot added (user-supplied)
- Sodium PyruvateNot added (user-supplied)
- pH (USP <791>)7.4
- Osmolality (USP <785>)280–320 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered RPMI passes particulates, aggregates and endotoxin fragments large enough to activate TLR4 signaling and confound sensitive immune cell assays. FluxMPS™ is built around a finer filtration train and a formulation tailored to lymphocyte and hematopoietic culture.
Microchannel-safe purity
0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate compliance support particle-free media for suspension immune cell OoC channels and flow cytometry workflows.
Immune cell–optimized formulation
RPMI 1640 carries reduced glutathione (antioxidant) and a balanced amino acid and vitamin profile developed for lymphocyte, T cell, and hematopoietic cell culture.
Ultrapure-grade water
Type 1 water, 18.2 MΩ·cm, supports a low trace-metal, low-TOC base for formulations used with signaling-sensitive immune cell assays.
Batch-controlled endotoxin
< 0.05 EU/mL release specification (LAL, USP <85>), tested per manufacturing batch to help limit LPS-driven activation artefacts in immune cell assays.
Rich, stable nutrient profile
RPMI 1640’s nutrient profile is developed for demanding immune and hematopoietic cell types, delivered particle-free through quadruple-stage 0.04 µm filtration.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two paired prefilter/final-filter passes — reaching a final 0.04 µm polish. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter, giving the train full redundancy rather than acting as a single descending cascade.
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1
0.1 µm Prefiltration I
Removes large aggregates and cell debris; mycoplasma-retentive grade (validated to A. laidlawii, typical mycoplasma diameter 0.2–0.3 µm); protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates below the resolution of standard 0.22 µm filtration.
-
3
0.1 µm Prefiltration II
Second dedicated mycoplasma-retentive prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish in validated ISO Class 5 (Class 100) laminar-flow conditions.
Filtration architecture vs. conventional media
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration of cell culture media.
© Diagnocine® — DCP-RPMI-QP1X
Immune cell OoC and hematopoietic applications
FluxMPS™ DCP-RPMI-QP1X — RPMI 1640 w/o L-glutamine, sodium pyruvate — delivers 0.04 µm filtered purity for lymphocyte, T cell, and hematopoietic OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic perfusion systems requiring the finest cut-off Diagnocine offers.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off of this Microfluidics Suitable product
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
T Cell & Lymphocyte Culture
RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration limits particulate exposure that can confound immune assays.
Leukemia & Lymphoma Lines
RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based media would alter proliferation and signaling.
Immune Cell OoC
0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip models built on microphysiological system (MPS) platforms.
CAR-T & TIL Expansion
A < 0.05 EU/mL endotoxin release specification supports CAR-T manufacturing and TIL expansion protocols where endotoxin control is a process priority.
Immune Cell Metabolic Flux
Defined RPMI base for lymphocyte metabolic flux analysis via ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Flow Cytometry & Confocal
Ultra-low particulate media suited to flow cytometry and confocal imaging of immune cells; a phenol red-free variant is available on request for fluorescence-sensitive channels.
Analytical release specifications
Every lot released against the full specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, Glucose (2.0 g/L) | [-] L-Glutamine, Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| Glucose | 2000 mg/L (2.0 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 280–320 mOsm/kg H2O |
| Total ingredients | 39 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO2 atmosphere recommended (buffered by 2.0 g/L sodium bicarbonate, pH 7.4) |
| Available sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
RPMI 1640: 39 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains reduced glutathione (antioxidant) and a balanced profile of vitamins and amino acids developed for lymphocyte and hematopoietic cell culture.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium nitrate tetrahydrate | 13477-34-4 | 100.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 48.840 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2000.000 |
| Sodium chloride | 7647-14-5 | 6000.00 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 800.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 10.000 |
| L-Arginine hydrochloride | 1119-34-2 | 241.000 |
| L-Asparagine | 70-47-3 | 50.000 |
| L-Aspartic acid | 56-84-8 | 20.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 65.200 |
| L-Glutamic acid | 56-86-0 | 20.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Hydroxyproline | 51-35-4 | 20.000 |
| L-Isoleucine | 73-32-5 | 50.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 40.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 15.000 |
| L-Proline | 147-85-3 | 20.000 |
| L-Serine | 56-45-1 | 30.000 |
| L-Threonine | 72-19-5 | 20.000 |
| L-Tryptophan | 73-22-3 | 5.000 |
| L-Tyrosine Disodium Salt | 69847-45-6 | 28.830 |
| L-Valine | 72-18-4 | 20.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 3.000 |
| D-Biotin | 58-85-5 | 0.200 |
| D-Ca-Pantothenate | 137-08-6 | 0.250 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 0.005 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Glutathione reduced | 70-18-8 | 1.000 |
| Phenol red sodium salt | 34487-61-1 | 5.300 |
| i-Inositol | 87-89-8 | 35.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feed water for trace-metal and TOC control.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 280–320 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-RPMI-QP1X compares
FluxMPS™ DCP-RPMI-QP1X vs. conventional 0.22 µm–filtered RPMI formulations.
| Parameter | DCP-RPMI-QP1X (FluxMPS™) | Conventional RPMI 1640 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm final cut-off) | Not specified | Not specified |
| RPMI 1640 without L-glutamine and sodium pyruvate — independent metabolic control for flux assays | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle 0.1 µm stage | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes (Method 1) | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-RPMI-QP1X — RPMI 1640 w/o L-glutamine, sodium pyruvate.
Supporting literature
Key publications supporting RPMI 1640 in immune cell culture and organ-on-a-chip applications.
- Moore GE, et al. RPMI 1640 medium for cultivation of human leukocytes. Cancer Res. 1966. doi:10.1002/jcp.1030700221
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Rothbauer M, et al. Recent advances in microfluidic technologies for immune cell studies. Lab Chip. 2018;18:249–270. doi:10.1039/c7lc00815e
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Parlato S, et al. 3D Microfluidic model for evaluating immunotherapy efficacy. Sci Rep. 2017;7:1093. doi:10.1038/s41598-017-01013-x
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
