FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ DCP-RPMIGH-QPBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 cell-culture medium engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. This high-glucose, 25 mM HEPES-buffered, bicarbonate-free and phenol-red-free formulation is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), finishing with a 0.04 µm final polish for microchannel-safe purity.

• RPMI 1640 base with high glucose (4500 mg/L D-Glucose) and 25 mM HEPES buffering
• Bicarbonate-free and phenol-red-free formulation; L-Glutamine and Sodium Pyruvate not added
• HEPES-buffered for reduced CO₂ dependence — validate per cell line
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
• Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) with a 0.04 µm final polish
• 37 defined components verified per lot with full CAS traceability
• Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa NJ
• Customization available: pH, glucose, HEPES, salts, and nutrients on request

Engineered where standard media fails

Conventional 0.22 µm–filtered media can pass mycoplasma-sized organisms, subvisible particulates, and aggregates that foul microfluidic channels and confound on-chip assays. FluxMPS™ addresses these failure modes through a 0.04 µm final-polish architecture and a fully defined, traceable formulation.

Quadruple-stage filtration system

Four serial filtration stages culminate in a 0.04 µm final polish under aseptic fill & finish, delivering media at a purity level beyond conventional 0.22 µm filtration.

1. 1

   0.1 µm Prefiltration I

   Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm final filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would pass.

3. 3

   0.1 µm Prefiltration II

   Second dedicated 0.1 µm prefilter, protecting the second 0.04 µm final filter cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish. Final product QC release gate.

OoC and MPS applications

FluxMPS™ DCP-RPMIGH-QPBR1X delivers ultra-filtered, HEPES-buffered RPMI 1640 for organ-on-a-chip, microfluidic, and metabolic research where particulate-free media and defined nutrient control are critical.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactors and robotic perfusion systems that demand the lowest possible particulate load — a distinct, higher tier above this Microfluidics Suitable (0.04 µm) product.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
• Valve & Sensor Protection: Reduces micro-fouling in automated perfusion and recirculation systems
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot is released against the product specification. A Certificate of Analysis is available: support@diagnocine.com.

Full composition (mg/L)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid — 37 ingredients verified per lot with CAS numbers for full raw-material traceability. All ingredients from the original formulation are preserved exactly.

ISO 13485 manufacturing & compliance

Every batch of FluxMPS™ DCP-RPMIGH-QPBR1X is manufactured under a certified ISO 13485:2016 QMS, with full lot traceability and multi-parameter QC release testing; final QC is performed at Diagnocine, Totowa NJ.

How DCP-RPMIGH-QPBR1X compares

FluxMPS™ versus conventional 0.22 µm filtered RPMI 1640 media for OoC and MPS applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIGH-QPBR1X and its use in OoC and MPS applications.

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and to HEPES-buffered RPMI 1640 cell-culture media.

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