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FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-RPMIGH-BR1X
$49.50
DCP-RPMIGH-BR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES (25mM) Contains Calcium Contains Magnesium Contains Glucose (High, 4500 mg/L) Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-RPMIGH-BR1X is a Microfluidics Suitable, ultra-filtered RPMI 1640 formulation engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration — this HEPES-buffered, bicarbonate-free, phenol-red-free formulation is purpose-built for imaging, metabolic flux analysis, and long-duration microfluidic culture.

  • High Glucose (4500 mg/L) RPMI 1640 base buffered with 25 mM HEPES
  • Bicarbonate-free, phenol-red-free formulation — compatible with Agilent Seahorse XF metabolic flux assays
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
  • L-Glutamine (300 mg/L) and Sodium Pyruvate (110 mg/L) included — ready for immediate culture
  • Manufactured under an ISO 13485:2016 quality management system
  • 39 verified ingredients with full CAS traceability, per-batch QC release
  • Custom pH, glucose, HEPES, and nutrient modifications available on request
CAT. NO.
DCP-RPMIGH-BR1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Formulation[+] High Glucose [+] L-Glutamine [+] Sodium Pyruvate [+] 25mM HEPES [-] Sodium Bicarbonate [-] Phenol Red
  • Glucose4500.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • CO₂ RequirementHEPES-buffered; reduced CO₂ dependence (validate per cell line)
  • Storage2–8°C, avoid light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that confound sensitive cell assays and foul microfluidic channels.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Formulated for OoC microfluidic chips where channel fouling is a design constraint.

target

Total metabolic control

High glucose, L-glutamine, and sodium pyruvate are already defined in this formulation, giving precise control over the carbon and nitrogen inputs feeding your experimental system.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content, supporting consistent, low-background culture chemistry.

visibility

Low background for imaging

Ultra-low particulate baseline reduces background scatter for confocal microscopy, biosensor, and live-cell imaging applications on-chip.

science

Rich, stable nutrient profile

39 verified ingredients per batch. Full CAS traceability. Micro-batch precision manufacturing with per-lot QC release.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter-plus-final-filter pairs — reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein clusters; protects the first 0.04 µm cartridge and extends its service life.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter allows through.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2’s effluent, but redundant protection for Stage 4.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish. Final product QC release gate.

Filtration architecture vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

0.04
µm final pore size
4
Filtration passes (two prefilter + final-filter pairs)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm / 0.04 µm mycoplasma-retentive filtration (mycoplasma diameter approximately 0.2–0.3 µm); this is a filtration control, not a per-lot mycoplasma test result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMIGH-BR1X quadruple-stage filtration system, 0.1 micron times two plus 0.04 micron times two, RPMI 1640 cell culture media for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-RPMIGH-BR1X.
© Diagnocine® — DCP-RPMIGH-BR1X
Applications

OoC and MPS Applications

FluxMPS™ DCP-RPMIGH-BR1X delivers a Microfluidics Suitable, HEPES-buffered, bicarbonate-free, phenol-red-free formulation for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems that demand the finest available cut-off.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion and recirculation systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip platforms, where particulate-free media prevents channel fouling.

OoCToCBoCLoCMPS
Cancer Biology

Cancer Cell Lines & Metabolic Research

Supports common cancer cell lines and Warburg effect metabolic studies with a high-glucose, defined nutrient background.

MCF-7MDA-MB-231HeLaJurkat
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered base for iPSC differentiation protocols requiring defined, particulate-free media.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particulate-controlled formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Bicarbonate-free, phenol-red-free formulation compatible with ¹³C isotope tracing, Agilent Seahorse XF real-time metabolic assays, and NMR metabolomics.

¹³C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate background and absence of phenol red support confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every batch released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow to colorless, clear solution (phenol red-free)
Total ingredients 39
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500.000 mg/L
Pack sizes 500 mL, 1000 mL
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement HEPES-buffered; reduced CO₂ dependence (validate per cell line)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid — 39 ingredients verified per batch with CAS numbers for raw-material traceability. All ingredients from the original formulation are preserved exactly.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 4500.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.000
Sodium pyruvate 113-24-6 110.000
Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-RPMIGH-BR1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every batch traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity feedwater with controlled trace-metal and TOC content, used throughout manufacturing.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in an ISO Class 5 (Class 100) cleanroom, immediately following the final 0.04 µm filtration pass.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-batch QC release. Each batch is tested independently — not pooled or blended across batches.

Endotoxin USP <85> BET

LAL-based endotoxin test performed per manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate analysis. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm.

Osmolality USP <785>

Osmolality verified per batch using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Full Certificate of Analysis available per batch. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIGH-BR1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter DCP-RPMIGH-BR1X (FluxMPS™) Conventional 0.22 µm Filtered Standard Alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES, [-] Bicarbonate, [-] Phenol Red Standard RPMI 1640 Standard RPMI 1640
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle cancel cancel
Water quality Type 1, 18.2 MΩ·cm Type 2 typical Type 2 typical
Manufacturing QMS ISO 13485:2016 ISO 9001 typical Variable
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIGH-BR1X and its use in OoC and MPS applications.

Yes. DCP-RPMIGH-BR1X is a Microfluidics Suitable RPMI 1640 formulation engineered for MPS and OoC platforms. The 0.04 µm final filtration reduces the risk of microfluidic channel fouling, and the bicarbonate-free, phenol-red-free formulation supports optical and biosensor readouts common in OoC, ToC, BoC, and LoC systems.
FluxMPS uses a quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration, with mycoplasma-retentive filtration at both 0.1 µm and 0.04 µm stages.
Sodium bicarbonate is excluded because this formulation is buffered with 25 mM HEPES instead, giving stable pH control outside a strictly regulated CO₂ incubator. Phenol red is excluded to eliminate optical interference in fluorescence, absorbance, and TEER-based assays. Glucose, L-glutamine, and sodium pyruvate are already included in this formulation and do not need to be added. If your protocol specifically requires bicarbonate buffering or a visual pH indicator, consider Diagnocine's bicarbonate-buffered, phenol-red-containing RPMI 1640 formulation instead.
No, not strictly. This bicarbonate-free, 25 mM HEPES-buffered formulation is designed for reduced CO₂ dependence and can be used in ambient air or in systems without tight CO₂ control. Some cell types and long-duration cultures may still perform better with a 5% CO₂ environment — validate empirically for your specific cell line and culture format.
Yes. This is a basal medium formulation. Supplement with FBS, serum replacements, or growth factors as required by your cell type and protocol. When filtering serum or protein-containing supplements after addition, use a 0.2 µm low-protein-binding PES or PVDF filter — a 0.04 µm membrane will strip serum of the proteins and lipoproteins it is added to provide and is not recommended for post-supplementation filtration.
The release specification is < 0.05 EU/mL, verified by the Limulus Amebocyte Lysate (LAL) assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) for every manufacturing batch, not per individual unit.
Yes. A CoA is available per batch upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin, sterility, mycoplasma filtration status, USP <788> particulate data, appearance, and full raw material lot traceability.
Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
  4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
  5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
  6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
  7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
  8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
  9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
  10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

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