UltraClean PVSA-MOPS, 1X
FluxMPS™ UltraClean PVSA-MOPS, 1X
A ready-to-use, MPS-grade 1X MOPS buffer engineered for RNase-safe handling of RNA and DNA. Supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor, and purified through quadruple-stage 0.1 µm and 0.04 µm membrane filtration (each applied twice) for ultra-clean, microchannel-safe performance in molecular biology and microfluidic workflows.
- Quadruple-stage filtration: 0.1 µm membrane (twice) and 0.04 µm membrane (twice) for ultra-clean, mycoplasma-safe purity
- Supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor
- Defined 20 mM MOPS buffering system at pH 7.4
- Manufactured with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm)
- Sterile, ready-to-use 1X formulation — no preparation required
- Manufactured under ISO 13485-certified, CE-approved facilities
- Customization available: alternate concentrations, additives, and pH on inquiry
- Catalog No.DCP-MOPSPVSA1X
- pH7.4
- Molarity (MOPS)20 mM
- RNase Inhibitor (PVSA)1 g/L
- Water QualityUltrapure, nuclease-free
- Filtration0.1 µm x2 + 0.04 µm x2
- SterilitySterile
- Storage2-8°C, do not freeze
- Shelf Life12 months
- FormatReady-to-use 1X
Engineered where standard MOPS buffers fail
Conventional 0.22 µm-filtered buffers can carry subvisible particulates, adventitious nucleases, and microbial bioburden that compromise RNA integrity and accumulate in microfluidic channels. UltraClean PVSA-MOPS, 1X addresses each failure mode directly with a defined buffering system, an active RNase inhibitor, and multi-stage sub-0.1 µm filtration.
Microchannel-Safe Purity
Sequential 0.1 µm and 0.04 µm membrane filtration, each stage applied twice, reduces particulate load beyond a single-pass 0.22 µm filtration commonly used in standard MOPS buffer.
Precise, Stable Buffering
Defined 20 mM MOPS concentration delivers consistent, near-physiological buffering at pH 7.4 for reproducible sample handling.
Ultrapure-Grade Water
Formulated with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm) to minimize background contamination in nucleic acid workflows.
RNase-Safe, Low-Background Handling
Polyvinylsulfonic acid (PVSA) provides robust, broad-spectrum RNase inhibition, reducing reliance on protein-based inhibitors during handling and short-term storage.
Defined, Traceable Composition
MOPS, PVSA, and water content are formulated to declared concentrations and manufactured under a controlled quality process.
Customization On Demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, or pH values available upon inquiry.
Quadruple-stage filtration system
Every lot of UltraClean PVSA-MOPS, 1X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice — a quadruple-stage architecture that supports ultra-clean, RNase-safe, mycoplasma-safe buffer for sensitive RNA and DNA workflows.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the service life of downstream filtration stages.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden ahead of the final sterile-filtration pass.
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3
0.1 µm Sterile-filtration I
Second-pass redundancy through a 0.1 µm membrane reinforces particulate and bioburden control.
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4
0.04 µm Sterile-filtration II — Final Polish
Final 0.04 µm polish, performed under an aseptic fill process, helps prevent mycoplasma contamination; the smallest known mycoplasma species is approximately 0.2 micron.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single-pass 0.22 µm filtration typically used in standard MOPS buffer preparations.
© Diagnocine® — DCP-MOPSPVSA1X
Where UltraClean PVSA-MOPS, 1X is used
Formulated for RNA and DNA resuspension, short-term storage, and general molecular biology procedures that specify a 1X MOPS buffer with added RNase protection.
Automated Bioreactors & Robotics
For automated, high-throughput microfluidic and robotic liquid-handling systems, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request to further minimize particulate load in sensitive valve and sensor pathways.
- Total Particulate Exclusion: Finer filtration threshold for the most particulate-sensitive automated workflows.
- Valve & Sensor Protection: Reduces the risk of particulate accumulation in microfluidic valves and inline sensors.
- Extended Perfusion Stability: Supports longer unattended run times in automated perfusion setups.
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade of this buffer.
Micro Physiological System (MPS) & Chip
MOPS-buffered, RNase-safe solution suitable for washing and handling steps in microfluidic chip-based systems where RNA integrity matters.
RNA & DNA Resuspension and Dilution
Resuspension and dilution of purified nucleic acids in a MOPS-based buffer with added RNase protection for storage or downstream analysis.
iPSC-Derived Model Handling
Near-neutral, zwitterionic buffering suitable for gentle handling steps in workflows involving iPSC-derived cell models where RNA quality is a priority.
Endothelial & Primary Cell Processing
Buffering solution for gentle handling or washing of cells and tissues in workflows where RNA integrity is important and MOPS is specified.
Sample Handling for Enzymatic Assays
Handling and transfer of nucleic acids prior to downstream applications such as RT, qPCR, and library prep, as compatible with enzyme- and salt-specific requirements.
General MOPS-Buffered RNA/DNA Procedures
Any procedure requiring 1X MOPS with enhanced RNA protection under controlled, near-physiological pH conditions.
Full specification sheet
Parameters as formulated and manufactured for UltraClean PVSA-MOPS, 1X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | MOPS 20 mM, PVSA 1 g/L, ultrapure nuclease-free water to volume |
| Appearance | Colorless, clear solution; free of visible particulates |
| pH (USP <791>) | 7.4 |
| Molarity / Concentration | MOPS 20 mM |
| Parameter | Specification |
|---|---|
| Filtration USP <788> | 0.1-micron membrane (twice) and 0.04-micron membrane (twice) |
| Sterility | Sterile |
| RNase Protection | PVSA (Polyvinylsulfonic Acid), 1 g/L |
| Water Quality USP <85> | Ultrapure, nuclease-free Type 1 water |
| Manufacturing Standard ISO | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2-8°C |
| Freeze-Thaw | Do not freeze |
| Shelf Life | 12 months |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Molecular biology-grade MOPS and PVSA |
| Manufacturing QMS ISO 13485 | ISO 13485-certified facility |
| Regulatory Alignment | CE-approved facility |
| Production Method | 0.1-micron membrane (twice) and 0.04-micron membrane (twice); ready-to-use 1X |
| Traceability | Final packaging, quality assurance, and customization performed at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
MOPS-buffered formulation supplemented with the polymeric RNase inhibitor PVSA; component concentrations are controlled per manufacturing lot.
| Component | CAS Number | Concentration |
|---|---|---|
| MOPS | 1132-61-2 | 20 mM |
| PVSA (Polyvinylsulfonic Acid) | 1 g/L | |
| Water (Ultrapure, nuclease-free) | 7732-18-5 | To volume |
Manufacturing & compliance
UltraClean PVSA-MOPS, 1X is manufactured under an ISO 13485-certified, CE-approved quality system, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified and CE-approved quality management system.
Ultrapure Type 1 Water
Formulated with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm).
ISO Class 5 Fill & Finish
Aseptic fill process supports the sterile, ready-to-use 1X format.
Micro-Batch Precision
Customization requests and assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA.
Filtration Verification USP <788>
Particulate control supported by 0.1-micron and 0.04-micron membrane filtration, each applied twice.
Sterility Assurance
Quadruple-stage membrane filtration helps prevent mycoplasma and microbial contamination.
Water Quality USP <85>
Manufactured with ultrapure, nuclease-free water to minimize background contamination.
Documentation / CoA
Lot-specific documentation is available upon request.
How DCP-MOPSPVSA1X compares
A side-by-side view of UltraClean PVSA-MOPS, 1X against conventional MOPS buffer preparations.
| Parameter | DCP-MOPSPVSA1X (FluxMPS™) | Conventional MOPS Buffer | Standard Alternative |
|---|---|---|---|
| RNase Protection (PVSA) | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Sterile | check_circle | cancel | check_circle |
| Water Quality | Ultrapure Type 1 | Standard grade | Standard grade |
| ISO 13485-Certified Manufacturing | check_circle | cancel | cancel |
| Microfluidic / OoC Compatibility | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about UltraClean PVSA-MOPS, 1X.
Supporting literature
Curated references relevant to MOPS buffer chemistry, RNase inhibition, and microfluidic RNA handling.
- Good, N.E., et al. Hydrogen ion buffers for biological research. Biochemistry. doi:10.1021/bi00866a011
- Good, N.E., Izawa, S. Hydrogen ion buffers. Methods in Enzymology. doi:10.1016/S0076-6879(72)24054-X
- Blackburn, P., Moore, S. Pancreatic ribonuclease. The Enzymes. doi:10.1016/S1874-6047(08)60406-5
- Chirgwin, J.M., et al. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. doi:10.1021/bi00591a005
- Bhattacharyya, D., et al. Polyanion-mediated inhibition of ribonucleases. Journal of Molecular Biology. doi:10.1016/0022-2836(92)90999-6
- Bhatia, S.N., Ingber, D.E. Microfluidic organs-on-chips. Nature Biotechnology. doi:10.1038/nbt.2989
- Zhang, B., et al. Organ-on-a-chip devices advance to market. Lab on a Chip. doi:10.1039/C6LC00450D
- Rio, D.C., et al. RNA: A Laboratory Manual. Cold Spring Harbor Laboratory Press. doi:10.1101/pdb.top078725
- Farrell, R.E. RNA Methodologies: Laboratory Guide for Isolation and Characterization. Academic Press. doi:10.1016/C2009-0-64150-2
- Halder, S., et al. Endotoxin and nuclease control in reagent manufacturing for cell-based assays. Applied Biosafety. doi:10.1177/1535676019878780
