UltraClean PVSA-HEPES, 1X
FluxMPS™ UltraClean PVSA-HEPES, 1X
UltraClean PVSA-HEPES, 1X is an MPS-grade, RNase-safe HEPES buffer supplied ready-to-use at pH 7.4 with 20 mM HEPES and polyvinylsulfonic acid (PVSA), a polymeric RNase inhibitor. Manufactured with quadruple-stage 0.1 µm and 0.04 µm membrane filtration and ultrapure Type 1 water, it is built for RNA and DNA handling in microfluidic, organ-on-a-chip, and molecular biology workflows.
- Sterile, ultrapure formulation — filtered 0.1-micron membrane twice and 0.04-micron membrane twice (quadruple-stage architecture)
- RNase-safe: supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor
- Precise, stable pH 7.4 with a defined 20 mM HEPES buffering system
- Manufactured with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm)
- Ready-to-use 1X working strength — no dilution or pH adjustment required
- Microchannel-safe purity for RNA/DNA resuspension, storage, and downstream molecular biology
- Manufactured under ISO 13485-certified, CE-approved facilities
- Customizable concentration, pH, and additives available on request
- pH7.4
- Buffering AgentHEPES, 20 mM
- RNase InhibitorPVSA, 1 g/L
- Water QualityUltrapure, nuclease-free (Type 1, 18.2 MΩ·cm)
- Filtration0.1 µm (x2) + 0.04 µm (x2), Quadruple-stage
- SterilitySterile
- AppearanceColorless, clear solution
- Storage2–8°C (do not freeze)
- Shelf Life12 months
- Sizes Available500 mL, 1000 mL
Engineered where standard RNA buffers fail
Conventional 0.22 µm-filtered buffers can carry subvisible particulates, adventitious nucleases, and microbial bioburden that compromise sensitive RNA workflows. UltraClean PVSA-HEPES, 1X is manufactured from molecular biology-grade HEPES and PVSA, finely filtered to minimize particulates, microbial contamination, and adventitious nucleases, so RNA integrity is protected from resuspension through downstream analysis.
Microchannel-safe purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration minimizes particulates and microbial contamination for microfluidic and chip-based RNA workflows.
Precise, stable pH
A defined 20 mM HEPES system delivers pH 7.4 buffering for near-physiological, RNA-compatible handling conditions.
Ultrapure-grade water
Formulated with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm) to eliminate adventitious nuclease carry-over.
RNase-safe by design
Polyvinylsulfonic acid (PVSA) provides robust inhibition of a broad spectrum of RNases, complementing nuclease-free technique and reducing reliance on protein-based inhibitors.
Defined, traceable composition
Every component — HEPES, PVSA, and ultrapure water — is manufactured and released to a fixed, documented formulation.
Customization on demand
Alternate concentrations, additional chemicals/compounds/proteins/supplements, different pH, and other modifications are available on inquiry.
Quadruple-stage filtration system
UltraClean PVSA-HEPES, 1X is sterile-filtered through a 0.1-micron membrane twice and a 0.04-micron membrane twice, a quadruple-stage architecture engineered to minimize particulates, bioburden, and adventitious nucleases for RNA- and DNA-sensitive workflows.
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1
0.1 µm Pre-filtration I
Large particulate and aggregate removal, extending the service life of downstream filters.
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2
0.04 µm Pre-filtration II
Retention of fine particulates and bioburden ahead of final sterile filtration.
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3
0.1 µm Sterile-filtration I
Second-pass 0.1-micron membrane redundancy to reinforce sterility assurance.
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4
0.04 µm Sterile-filtration II — Final Polish
Second-pass 0.04-micron membrane polish. Mycoplasma organisms are typically about 0.2 microns in size, so this final polish supports mycoplasma-controlled, RNase-safe buffer for RNA and DNA handling.
Performance vs. conventional buffer
Where conventional laboratory buffers commonly rely on a single 0.22-micron filtration pass, UltraClean PVSA-HEPES, 1X undergoes sequential 0.1-micron and 0.04-micron membrane filtration, each applied twice, to reduce particulates and bioburden beyond what single-stage filtration achieves and to support RNase-safe, mycoplasma-controlled handling of RNA and DNA samples.
© Diagnocine® — DCP-HEPESPVSA1X
Built for RNA-sensitive workflows
UltraClean PVSA-HEPES, 1X is formulated for RNA and DNA resuspension, RNA handling and short-term storage, sample preparation for enzymatic assays, cell and tissue processing, and general molecular biology procedures where 1X HEPES with enhanced RNA protection is required under controlled, near-physiological pH conditions.
- RNA and DNA Resuspension: Resuspension and dilution of nucleic acids in a HEPES-based buffer with added RNase protection for storage or downstream analysis.
- RNA Handling and Short-Term Storage: Temporary storage of RNA-containing samples, lysates, or purified RNA where a zwitterionic, near-neutral buffer is preferred.
- Sample Preparation for Enzymatic Assays: Handling and transfer of nucleic acids prior to RT, qPCR, or library prep, as compatible with the specific enzyme and salt conditions.
- Cell and Tissue Processing: Buffering solution for gentle handling or washing of cells and tissues where RNA integrity is important and HEPES is specified.
- General Molecular Biology: Any procedure requiring 1X HEPES with enhanced RNA protection under controlled, near-physiological pH.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on inquiry to further protect sensitive valves, sensors, and perfusion lines.
- Total Particulate Exclusion: Ultra-fine filtration to protect automated fluid paths.
- Valve & Sensor Protection: Reduced particulate load supports long-term instrument reliability.
- Extended Perfusion Stability: Cleaner buffer supports more consistent automated perfusion runs.
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.
Micro Physiological System (MPS) & Chip
RNase-safe HEPES buffering for RNA/DNA handling within chip-based culture and perfusion systems.
RNA/DNA Resuspension & Dilution
Resuspension, dilution, and transfer of nucleic acids ahead of downstream analysis, RT, qPCR, or library prep.
iPSC-Derived Model Handling
Near-physiological, RNase-safe buffering for RNA-sensitive handling of stem cell-derived models.
Endothelial & Primary Cell Processing
Gentle HEPES-buffered handling and washing of cells and tissues where RNA integrity is important.
ELISA, Blotting & Blocking Support
Compatible with protocols specifying HEPES as the buffering system in general molecular biology workflows.
Microscopy & Optical Sensing
Zwitterionic, near-neutral buffer suited to RNA-containing samples during live-cell imaging workflows.
Verified performance parameters
Specifications for UltraClean PVSA-HEPES, 1X as manufactured and released by Diagnocine.
| Parameter | Specification |
|---|---|
| Formulation / Composition | HEPES 20 mM, PVSA 1 g/L, ultrapure nuclease-free water to volume |
| Appearance | Colorless, clear solution; free of visible particulates |
| pH USP <791> | 7.4 |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Sterile |
| Nuclease Status | RNase-safe (PVSA inhibitor); nuclease-free water |
| Water Purity | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2–8°C |
| Freeze-Thaw | Do not freeze |
| Shelf Life | 12 months |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Molecular biology-grade HEPES and PVSA |
| Manufacturing QMS ISO 13485 | ISO 13485-certified, CE-approved facilities |
| Production & QA Site | Final packaging, QA, and testing at the Diagnocine R&D and Quality Testing Center; customization assembled at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
UltraClean PVSA-HEPES, 1X is a defined HEPES buffering system supplemented with polyvinylsulfonic acid (PVSA) for RNase protection.
| Component | CAS Number | Concentration |
|---|---|---|
| HEPES | 7365-45-9 | 20 mM |
| PVSA (Polyvinylsulfonic Acid) | 1 g/L | |
| Water (Ultrapure, nuclease-free) | 7732-18-5 | To volume |
Manufactured under controlled conditions
UltraClean PVSA-HEPES, 1X is manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing completed at the Diagnocine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified, CE-approved facilities (suppliers of Diagnocine Precision).
Ultrapure Type 1 Water
Formulated with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm).
Quadruple-Stage Filtration
Filtered 0.1-micron membrane twice and 0.04-micron membrane twice for RNase-safe, mycoplasma-controlled purity.
Micro-Batch Precision
All specific customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.
Sterility USP <71>
Product is manufactured and released as sterile.
Appearance Check
Verified colorless, clear solution; free of visible particulates.
pH USP <791>
Released at pH 7.4.
Documentation / CoA
Certificate of Analysis available on request.
How DCP-HEPESPVSA1X compares
A qualitative comparison of UltraClean PVSA-HEPES, 1X against conventional and standard HEPES buffer alternatives.
| Parameter | DCP-HEPESPVSA1X (FluxMPS™) | Conventional HEPES Buffer | Standard Alternative |
|---|---|---|---|
| RNase Inhibition | check_circle PVSA polymeric inhibitor | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Defined HEPES Molarity | check_circle 20 mM | check_circle | cancel |
| Water Quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified | Standard purified |
| Manufacturing QMS | check_circle ISO 13485:2016 | cancel | cancel |
| Microfluidic Channel Compatibility | check_circle | cancel | cancel |
| Custom Formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about UltraClean PVSA-HEPES, 1X.
Supporting literature
Curated literature relevant to HEPES buffering, RNase inhibition, and microfluidic/organ-on-a-chip RNA handling.
- Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Ferreira GN, et al. Anionic polymer inhibitors of ribonuclease. Biotechnol Prog. 2000. doi:10.1021/bp0000239
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Zhang B, et al. Organ-on-a-chip devices for drug discovery. Nat Rev Mater. 2018. doi:10.1038/s41578-018-0034-7
- Fabre AL, et al. RNA integrity considerations in sample handling. BMC Mol Biol. 2014. doi:10.1186/1471-2199-15-3
- Ferreira GN, et al. Ribonuclease inhibitors in molecular biology applications. Trends Biotechnol. 2001. doi:10.1016/S0167-7799(01)01798-1
- Low LA, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021. doi:10.1038/s41573-020-0079-3
- Wagner I, et al. A dynamic multi-organ-chip for cell culture. Lab Chip. 2013. doi:10.1039/C3LC50234A
