UltraClean PVSA-HEPES, 1X
Cat No.:
DCP-HEPESPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-HEPES, 1X is a ready-to-use, high-purity HEPES buffer formulated to be RNase-safe for demanding RNA and DNA workflows at near-physiological pH. It is supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, processing, and short-term storage. The buffer provides the well-defined buffering characteristics of 1X HEPES at physiological pH, making it suitable for use with purified nucleic acids, RNA-containing samples, and cells in downstream molecular biology applications.
Applications:
- RNA and DNA Resuspension: Resuspension and dilution of nucleic acids in a HEPES-based buffer with added RNase protection for storage or downstream analysis.
- RNA Handling and Short-Term Storage: Temporary storage of RNA-containing samples, lysates, or purified RNA where a zwitterionic, near-neutral buffer is preferred.
- Sample Preparation for Enzymatic Assays: Handling and transfer of nucleic acids prior to downstream applications (e.g., RT, qPCR, library prep), as compatible with the specific enzyme and salt conditions.
- Cell and Tissue Processing: Buffering solution for gentle handling or washing of cells and tissues in workflows where RNA integrity is important and HEPES is specified.
- General Molecular Biology: Any procedure requiring 1X HEPES with enhanced RNA protection under controlled, near-physiological pH conditions.
Advantages:
- RNase-Safe: PVSA provides robust inhibition of a broad spectrum of RNases, complementing nuclease-free techniques and reducing reliance on protein-based inhibitors.
- High Purity: Manufactured from molecular biology-grade HEPES and PVSA and finely filtered to minimize particulates, microbial contamination, and adventitious nucleases.
- Versatile Usage: Compatible with protocols that specify HEPES as the buffering system for RNA, DNA, or cell-based workflows.
- Ready-to-Use 1X Solution: Supplied at working strength to reduce preparation time, variability, and contamination risk.
Composition:
| Ingredients |
Concentration |
| HEPES |
20 mM |
| PVSA (Polyvinylsulfonic Acid) |
1g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
