NanoDisc BD Kit

NanoDisc BD Kit


Cat. No.: FNK-CFS-CNDE-BD
Size: 1 Kit
This Product has a shelf life of 1 year being safely stored at -80°C.
CFS products are for research use only.


Description: 

Comes as a set with reagents necessary for NanoDisc synthesis for membrane protein Perfectly suited to NanoDisc synthesis of membrane protein with good quality and yield

Features: 

• NanoDisc has been synthesized using co-expression with Asolectine liposome, mRNA for MSP ( Membrane scaffold protein) synthesis and mRNA for target membrane protein
• Can be used for 6 reactions on 2ml of bilayer–dialysis method which has been established for proteoliposome sysnthesis
• Target proteins can be confirmed through SDS-PAGE/CBB staining.

Recommended for: 

• Users who require yields of membrane proteins several hundred micrograms.
• Users who want to perform interaction analysis
• Users who want to perform structure analysis of membrane protein

* This kit does not include dialysis device. A separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.) is required.
  

Flow of operations: 
 

Product Composition

1. NanoDisc BD Kit (white box):
 

Reagent Name	Concentration	Volume	Quantity
Transcription Reaction
SP6 RNA Polymerase	80K unit/mL	15 µL	1
RNase Inhibitor	80K unit/mL	15 µL	1
NTPmix	25 mM	120 µL	1
5×Transcription Buffer LM	5x	240 µL	1
Translation reaction
WEPRO7240ND	240OD	1mL	1
SUB-AMIX SGC S1**	40x	7.6 mL	1
SUB-AMIX SGC S2**	40x	7.6 mL	1
SUB-AMIX SGC S3**	40x	7.6 mL	1
SUB-AMIX SGC S4**	40x	7.6 mL	1
Creatine Kinase	20 mg/mL	20 µL	1

2. ND Plasmid set (white box):
 

Reagent Name	Concentration	Volume	Quantity
pEU-E01-MCS-TEV-SBP-C1	1 mg/ml	10ul	1
pEU-E01-MSP	1 mg/ml	85ul	1
pEU-E01-CLDN4-SBP	1 mg/ml	10ul	1

3. SA set (white box):
 

Reagent Name	Concentration	Volume	Quantity
SUB-AMIX SGC S1**	40x	7.6 mL	1
SUB-AMIX SGC S2**	40x	7.6 mL	1
SUB-AMIX SGC S3**	40x	7.6 mL	1
SUB-AMIX SGC S4**	40x	7.6 mL	1
Creatine Kinase	20 mg/mL	20 µL	1
Asolectin Liposome, lyophilized	-	10 mg	6
pEU-E01-Claudine4（Positive Control）	1 µg/µL

4. Asolectin liposome, lyopphilized (green box):
 

Reagent Name	Concentration	Volume	Quantity
Asolectin Liposome, lyophilized	-	10 mg	6

Storage Condition :  -80 ℃
 

*WEPRO7240ND is based on WEPRO7240 which dedicated for NanoDisc synthesis

**SUB-AMIX SGC S1-S4 each vary in their composition.

  When producing a 1x concentration, adjust by adding the same quantity of each.
 

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