FLEXIQuant PLUS Expression Kit

Product#: FNK-CFS-FLEX-MS

$2,204.73

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FLEXIQuant PLUS Expression Kit

Cat. No.: FNK-CFS-FLEX-MS
Size: 1 Kit
This Product has a shelf life of 1 year being safely stored at -80°C.
CFS products are for research use only.

Description:

This kit was designed to be user-friendly even for first-time users.
Our wheat germ cell-free expression system allows you to easily make full-length isotope-labeled MS standards.

Features:

Incorporates stable ¹³C and ¹⁵N isotopes-labeled lysine and arginine residues suitable for working with trypsin digestion in bottom-up proteomics
Very high labeling efficiency with incorporation rates of over 99%
No need to purchase ¹³C and ¹⁵N stable isotope-labeled amino acids as they are already added to translation system
Special expression and control vector provided to utilize FLEX-tag for absolute protein quantification by FLEXIQuant method
All reagents included to directly prepare labeled proteins from DNA template
Premixed reagents to synthesize proteins with just a few pipetting steps
Translation buffer provided in special reaction cups for bilayer reactions
Target protein expression can be confirmed through SDS-PAGE/CBB staining
Kit contains reagents for 8 translation reactions on 226 µl reaction scale

Recommended for:

Users who require full-length isotope-labeled MS standards easily using wheat germ cell-free expression system

All reagents are already aliquoted to provide the amounts needed per reaction.
This makes reaction setup easy and avoids unnecessary freeze-thawing of sensitive reagents.
Simply cut off the number of tubes needed for each experiment.

Transcription reaction mixture (Transcription Buffer LM Premix)