SUB-AMIX SG

SUB-AMIX SG


Cat. No.: FNK-CFS-SUB-SG
Product Size: 4 times 12.5 ml
Concentration: 40 x working concentration
Storage: -20°C


Description: 

Translation Buffer dedicated for WEPRO7240, WEPRO7240G and WEPRO7240H.
This product optimized for Filter＆Feed protein synthesis method. 

ONLY use translation buffer SUB-AMIX-SG when working with the Protemist XE Instrument! The translation buffer must be used in combination with our wheat germ extracts WEPRO®7240/WEPRO®7240G/WEPRO®7240H and Transcription Buffer LM! The translation buffer SUBAMIX-SG is provided as a set of four different 40 times stock solutions (S-1, S-2, S-3, S-4).

To prepare 400 ml of 1x SUB-AMIX® SG translation buffer, add 10 ml of each S-1 through S-4 stock solution to 360 ml of nuclease-free water (*) while stirring the solution. DO NOT mix the stock solutions directly as the amino acids will precipitate. To avoid multiple freeze-thawing cycles, subdivide 1x SUB-AMIX® SG translation buffer into appropriate aliquots before storing them at -20°C.

Note (*): We DO NOT recommend homemade DEPC treated water.
Refer to our protocols for more information on how this product can be used in transcription reactions and in combination with our wheat germ cell-free protein expression system.

Shipment and Storage

Our products are shipped on dry ice. Store the reagents at -20°C upon arrival. The reagents are temperature sensitive and should be always stored in a freezer at -20°C. Keep reagents on ice while using them at the bench. Avoid unnecessary freeze/thawing.