FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ DCP-MEMH-BR1X is an MPS-grade, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for Primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. NEAA pre-loaded — reduces metabolic burden on primary cells. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Sodium Bicarbonate, [-] Phenol Red.

Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high glucose.
NEAA included reduces metabolic burden and ammonia from de novo synthesis
25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂; ideal for open-air handling, flow cytometry prep, and atmospheric incubation
0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin for sensitive primary and hematopoietic cell cultures
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill

DCP-MEMH-BR1X | Size: 500 mL and 1000 mL| UNSPSC 41122100| Cell Culture Media

Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Media familyMEM Low Glucose + NEAA + 25mM HEPES
Glucose1000 mg/L (1.0 g/L, Low Glucose)
HEPES25 mM, pKa 7.3 at 37°C
NEAAPresent
Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES
[-] Sodium Bicarbonate, [-] Phenol Red
AppearancePale Yellow-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)235.00-275.00 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 μm ×2 + 0.04 μm ×2

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 μm filtered MEM passes mycoplasma, particulates, and endotoxin that alter primary cell phenotype. FluxMPS™ eliminates these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-free primary cell media prevents chip channel clogging and mycoplasma-driven phenotypic drift.

biotech

Primary cell—optimized formulation

MEM + Earle's Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

HEPES: CO₂-stable pH

25 mM HEPES prevents pH rise during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin — below the TLR4 activation threshold. For primary cells, prevents LPS-driven fibroblast activation, neuroinflammation, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, improving viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 μm Pre-filtration I — Large Particulate Removal

Removes large aggregates; protects downstream 0.04 µm membranes.
2

0.04 μm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1-0.3 µm) — absent from standard 0.22 µm filtration.
3

0.1 μm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 μm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEMH-BR1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered media, with confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

μm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.

FluxMPS™ DCP-MEMH-BR1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Pre-filtration I, 0.04 μm Pre-filtration II mycoplasma barrier, 0.1 μm Sterile-filtration I, 0.04 μm Final Polish ? MPS-grade MEM Low Glucose + NEAA + 25mM | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-BR1X

Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-BR1X is purpose-built for Primary fibroblasts and related cell models with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Eliminates micro-fouling in delicate chip geometries
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is the physiological-closest base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip

Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip

Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is the classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts

Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM prevents particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate; phenol red-free variants eliminate autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES \| [-] Sodium Bicarbonate, [-] Phenol Red
Appearance	Pale Yellow-colored, clear solution
Glucose	1000 mg/L (1.0 g/L, Low Glucose)
HEPES	25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA	Present
pH USP <791>	7.4
Osmolality USP <785>	235.00-275.00 mOsm/kg H₂O
Total ingredients	35

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2-8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	Cold pack
CO₂ requirement	CO₂-independent — HEPES (25 mM) alone maintains pH 7.2-7.4 at 37°C without gas supplementation

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 35 ingredients verified per lot with CAS numbers. NEAA listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) listed under OTHERS.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	265.000
Magnesium sulphate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium chloride	7647-14-5	6800.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	122.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	7.500
L-Alanine	56-41-7	8.900
L-Arginine hydrochloride	1119-34-2	126.000
L-Asparagine monohydrate	5794-13-8	15.000
L-Aspartic acid	56-84-8	13.300
L-Cystine dihydrochloride	30925-07-6	31.300
L-Glutamic acid	56-86-0	14.700
L-Glutamine	56-85-9	292.000
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	52.000
L-Leucine	61-90-5	52.000
L-Lysine hydrochloride	657-27-2	72.500
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	32.000
L-Proline	147-85-3	11.500
L-Serine	56-45-1	10.500
L-Threonine	72-19-5	48.000
L-Tryptophan	73-22-3	10.000
L-Tyrosine disodium salt dihydrate	69847-15-0	51.900
L-Valine	72-18-4	46.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	1.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	1.000
Nicotinamide	98-92-0	1.000
Pyridoxal hydrochloride	65-22-5	1.000
Riboflavin	83-88-5	0.100
Thiamine hydrochloride	67-03-8	1.000
i-Inositol	87-89-8	2.000
OTHERS
D-Glucose	50-99-7	1000.000
HEPES	7365-45-9	5958.000
Sodium pyruvate	113-24-6	110.000

Custom formulation: Contact support@diagnocine.com for DCP-MEMH-BR1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235.00-275.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEMH-BR1X lot at support@diagnocine.com.

Product Comparison

How DCP-MEMH-BR1X compares

FluxMPS™ DCP-MEMH-BR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA + formulations.

Parameter	DCP-MEMH-BR1X (FluxMPS™)	Conventional MEM Low Glucose (0.22 μm filtered)	Standard DMEM (0.22 μm)
MEM + NEAA + HEPES-only CO₂-free - no Phenol Red; atmospheric incubation, autofluorescence-free	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 μm	0.22 μm	0.22 μm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 μm)	cancel No	cancel No
HEPES (25 mM) included	check_circle Yes	cancel Usually no	cancel No
NEAA included	check_circle Yes	Optional add-on	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-BR1X.

DCP-MEMH-BR1X is processed through a Quadruple-stage filtration system reaching 0.04 µm, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with sub-mycoplasma filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.

FluxMPS™ uses four sequential filters ? 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish ? resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

HEPES alone provides CO₂-independent pH buffering; phenol red removed for autofluorescence-free imaging. Creates a fully CO₂-independent, optically clean primary cell base with L-Glutamine and pyruvate pre-loaded. Ideal for open-top chips with fluorescence detection and atmospheric incubation.

CO₂-independent ? HEPES (25 mM) alone maintains pH 7.2?7.4 at 37°C without gas supplementation.

NEAA are pre-loaded, providing non-essential nitrogen and carbon skeleton support. This reduces the metabolic burden on primary cells that must synthesize these amino acids de novo from glutamine and glucose improving viability in low-serum conditions and reducing ammonia accumulation.

FluxMPS™ DCP-MEMH-BR1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For primary cells, endotoxin activates TLR4, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432-437. doi:10.1126/science.130.3373.432
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117-129. doi:10.1016/j.biomaterials.2018.07.014
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid