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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-BR1X
$49.50
DCP-MEMH-BR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-MEMH-BR1X is a Microfluidics Suitable, ultra-filtered MEM Low Glucose + NEAA + 25 mM HEPES formulation engineered for primary fibroblasts and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering in this sodium-bicarbonate-free, phenol-red-free formulation.

  • Low Glucose (1.0 g/L, 1000 mg/L) — a physiological carbon source suited to primary cells sensitive to high-glucose media
  • NEAA pre-loaded (Gly, Ala, Asn, Asp, Glu, Pro) — reduces de novo synthesis burden and ammonia accumulation in primary cultures
  • 25 mM HEPES (pKa 7.3 at 37°C), sodium-bicarbonate-free and phenol-red-free — CO2-independent pH buffering for open-air handling and imaging workflows
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Available in 500 mL and 1000 mL pack sizes
  • Custom pH, glucose concentration, salts, and HEPES levels available on request — contact support@diagnocine.com
DCP-MEMH-BR1X · Sizes: 500 mL, 1000 mL · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25 mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] HEPES (25 mM) | [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235 - 275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM carries particulates and mycoplasma-sized organisms that a coarser filter does not retain, which can alter primary cell phenotype. FluxMPS™ addresses these failure modes through validated filtration while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 particulate compliance help keep media particle counts low, reducing the risk of chip channel obstruction in narrow microfluidic geometries.

biotech

Primary cell—optimized formulation

MEM + Earle's Salts + NEAA is an established base for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO2-stable pH

25 mM HEPES helps prevent pH drift during open-air suspension handling, flow cytometry preparation, and multi-well assay setup performed outside a CO2 incubator.

shield

Low endotoxin specification

Endotoxin activates TLR4 signaling in many primary cell types; the < 0.05 EU/mL release specification is intended to minimize this risk factor in primary fibroblast, neuronal, and epithelial cultures.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, which may improve viability in low-serum conditions and reduce ammonia accumulation.

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Customization on demand

pH, glucose, and HEPES concentration modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter does not.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill and finish.

Performance vs. conventional media

5×
Lower particulate count vs. 0.22 µm media
0.04
µm final pore size, four filtration passes
Sterility & mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma (0.2–0.3 µm) risk is mitigated by 0.1 µm mycoplasma-retentive filtration; this is a filtration control, not a per-lot mycoplasma test unless separately reported on the Certificate of Analysis.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMH-BR1X Minimum Essential Medium (MEM) Low Glucose NEAA 25mM HEPES Quadruple-stage filtration system 0.1 micron times 2 plus 0.04 micron times 2 for organ-on-a-chip and microfluidic applications Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-BR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-BR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade, ultra nano-filtered variant of this formulation is available on request for automated bioreactor and robotic liquid-handling platforms.

  • Total particulate exclusion: 0.01 µm filtration removes nanoparticulate aggregates not retained at 0.04 µm
  • Valve & sensor protection: reduces micro-fouling risk in delicate chip geometries
  • Extended perfusion stability: supports consistent nutrient delivery over long-duration culture

Inquiry required: contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle's Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity assessment in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle's salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps reduce particulate clogging risk in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Low background for imaging

The 0.04 µm final filtration provides an ultra-low particulate baseline suited to confocal microscopy, TEER, and biosensor applications on primary-cell chips. Phenol red is excluded to reduce optical interference at visible wavelengths for absorbance-based assays.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] HEPES (25 mM) | [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 235 - 275 mOsm/kg H2O
Total ingredients 36 across 3 composition tabs (Inorganic Salts, Amino Acids, Vitamins & Others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification, per batch)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protected from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — 25 mM HEPES maintains stable pH at 37°C without gas-phase CO2 supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25 mM HEPES: 36 ingredients verified per lot with CAS numbers where known. NEAA components are listed under AMINO ACIDS; HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-BR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm resistivity feed water; controlled for trace metals and total organic carbon.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235 - 275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-BR1X compares

FluxMPS™ DCP-MEMH-BR1X vs. conventional 0.22 µm-filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-BR1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm) Standard (0.22 µm) Standard (0.22 µm)
Bicarbonate-free, phenol-red-free, HEPES-buffered NEAA formulation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle 0.1 µm stage cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Higher clogging risk cancel Higher clogging risk
Custom formulation check_circle Available cancel Fixed formulation cancel Fixed formulation

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-BR1X.

Yes. DCP-MEMH-BR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, giving ultra-low particulate levels suited to OoC, MPS, and LoC platforms. The MEM + Earle's Salts + NEAA formulation with sub-mycoplasma-scale filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
 
HEPES alone provides CO2-independent pH buffering, so sodium bicarbonate is not required; phenol red is excluded to reduce optical interference in absorbance- and fluorescence-based assays. This creates a bicarbonate-free, phenol-red-free primary-cell base with L-glutamine and sodium pyruvate pre-loaded, suited to open-top chips with fluorescence detection and atmospheric incubation. If your protocol requires a bicarbonate/CO2 buffered or phenol-red-containing format, contact support@diagnocine.com for a custom formulation.
No. This formulation is CO2-independent: 25 mM HEPES (pKa 7.3 at 37°C) buffers pH without gas-phase CO2 supplementation, making it suitable for atmospheric incubation, open-well handling, and off-line processing steps such as flow cytometry preparation.
Yes. When adding serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — do not use a 0.04 µm membrane, which retains IgM, lipoproteins, and much of the functional serum fraction. Defined, protein-free additions may be filtered at 0.1 µm.
The release specification is < 0.05 EU/mL, verified by LAL assay (USP <85>) on every manufacturing batch prior to release; assay sensitivity is 0.005 EU/mL. This is a batch-level release specification, not a per-unit certificate.
Yes. A full CoA per batch covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25 mM HEPES in primary cell and OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432-437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117-129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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