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FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMGH-QBRN1X
$44.00
DCP-MEMGH-QBRN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (4.5 g/L) Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. Processed through a validated four-stage filtration train (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final cut-off — substantially finer than conventional 0.22 µm-filtered media — making it microchannel-safe from day one.

  • Bicarbonate-free, phenol-red-free, L-glutamine-free MEM High Glucose formulation buffered with 25 mM HEPES for reduced CO₂ dependence
  • High glucose (4.5 g/L / 4500 mg/L) plus sodium pyruvate (110 mg/L) support high-energy-demand cell types
  • Quadruple-stage ultra-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II) reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET)
  • Manufactured under an ISO 13485:2016 quality management system with aseptic fill & finish
  • pH 7.4 (USP <791>); osmolality reported on the Certificate of Analysis
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMGH-QBRN1X | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variation that accumulate inside microchannels — corrupting biosensor readings, stressing sensitive primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes at the source through validated quadruple-stage filtration.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles down to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

High-glucose metabolic support

4.5 g/L glucose plus 110 mg/L sodium pyruvate support high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm per ASTM D1193), minimizing trace-metal and organic-carbon (TOC) contribution to the finished medium.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements in tight microfluidic geometries.

science

Rich, stable nutrient profile

Full amino acid and vitamin coverage plus micro-batch precision ensures reproducible nutrient consistency for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid is processed through a four-stage serial filtration sequence — two dedicated 0.1 µm mycoplasma-retentive prefilters paired with two 0.04 µm final filters — reaching a 0.04 µm final cut-off substantially finer than standard 0.22 µm filtration.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates while providing 0.1 µm mycoplasma-retentive filtration; protects the downstream 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm mycoplasma-retentive prefilter, protecting the second 0.04 µm cartridge and providing full-train redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production lot.

5×
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration (not tested per lot). No bacterial or fungal growth observed.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid (DCP-MEMGH-QBRN1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-MEMGH-QBRN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid is validated for organ-on-a-chip, cancer biology, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional MPS Grade, 0.01 µm (10 nm) ultra nano-filtered variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the MPS Grade, 0.01 µm variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure, HEPES-buffered formulation supports sensitive iPSC differentiation protocols where endotoxin variation causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER stability in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose, bicarbonate-free base enables precise ¹³C isotope tracing and glycolytic flux experiments. Compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load and phenol red-free formulation are ideal for high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment. Available pack sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Formulation MEM High Glucose, 25 mM HEPES; without L-Glutamine, Sodium Bicarbonate, Phenol Red
Appearance Pale yellow, clear solution (phenol red-free)
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not present — supplement as needed
Sodium Pyruvate 110 mg/L
Phenol Red Not present
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement HEPES-buffered; reduced CO₂ dependence (validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Total: 28 components across 4 categories. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
Sodium Pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

filter_alt

Quadruple-stage filtration

0.1 µm ×2 + 0.04 µm ×2 sequential filtration train reaching a 0.04 µm final cut-off on every lot.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Per-Lot QC & CoA

Micro-batch manufacturing with full lot-release testing and Certificate of Analysis for every batch.

Endotoxin — USP <85> BET

LAL assay on every batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEMGH-QBRN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.

Parameter DCP-MEMGH-QBRN1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation trait Bicarbonate-free, phenol red-free, L-glutamine-free; 25 mM HEPES Sodium bicarbonate-buffered, phenol red present Sodium bicarbonate-buffered, phenol red present
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMGH-QBRN1X uses our Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that prevent microchannel clogging in OoC and MPS devices.
Standard 0.22 µm filtration leaves intact mycoplasma (0.2–0.3 µm) and subvisible particulates that accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1.
L-Glutamine is omitted so users can add it fresh (or a stable dipeptide substitute) at the concentration required by their cell line immediately before use. Sodium Bicarbonate and Phenol Red are excluded because this formulation is buffered with 25 mM HEPES for reduced CO₂ dependence and is free of phenol red, which interferes with optical and fluorescence-based assays. If you require a bicarbonate-buffered or phenol red-containing version, contact support@diagnocine.com for a custom formulation.
This formulation is HEPES-buffered (25 mM) and bicarbonate-free, reducing CO₂ dependence. It can be used in ambient-air or reduced-CO₂ environments; validate incubation conditions for your specific cell line.
Yes. This medium can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Add supplements immediately before use. When filtering serum or protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter — never 0.04 µm, which retains serum proteins and lipoproteins and will clog rapidly.
Endotoxin is controlled per manufacturing batch by LAL assay per USP <85> BET, with a release specification of < 0.05 EU/mL and assay sensitivity of 0.005 EU/mL. Every batch is tested before release; batch-specific results are documented in the CoA, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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