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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMGH-PBR1X
$49.50
DCP-MEMGH-PBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (High) Without Sodium Bicarbonate Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEMGH-PBR1X is a Microfluidics Suitable, ultra-filtered MEM High Glucose + NEAA + 25mM HEPES formulation engineered for primary neurons and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust CO2-independent pH buffering.

  • High Glucose (4.5 g/L) supports energy-demanding primary neuronal, cardiac and epithelial cell types
  • NEAA pre-loaded — reduces de novo synthesis burden and ammonia accumulation in primary cell culture
  • 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering for open-air handling, flow cytometry prep, and atmospheric incubation
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
  • Endotoxin release specification < 0.05 EU/mL (USP <85> LAL assay) — relevant to TLR4-sensitive primary cell cultures
  • Formulated without sodium pyruvate, sodium bicarbonate, or phenol red — a clean CO2-free base for atmospheric incubation and optical/biosensor detection
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
DCP-MEMGH-PBR1X Sizes: 500 mL, 1000 mL Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM High Glucose + NEAA + 25mM HEPES
  • Formulation[+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a foundational medium for primary cell biology — but conventional 0.22 µm filtered MEM can pass mycoplasma, particulates and endotoxin that alter primary cell phenotype. FluxMPS™ targets these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media helps reduce chip channel clogging risk.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA is an established base for primary fibroblasts, neurons, epithelial and vascular cells.

water_drop

HEPES: CO2-stable pH

25 mM HEPES helps limit pH drift during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO2 incubators.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — below the TLR4 activation threshold cited in the literature. For primary cells this is relevant to limiting LPS-driven fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis demand on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish.

Performance vs. conventional media

5×
Targeted reduction in particulate count vs. 0.22 µm media
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control is by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMGH-PBR1X Minimum Essential Medium (MEM) High Glucose NEAA 25mM HEPES w/o Sodium Pyruvate Sodium Bicarbonate Phenol Red 1X Liquid - Quadruple-stage filtration system 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMGH-PBR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMGH-PBR1X is intended for primary neuron and related primary cell models, with 0.04 µm filtration purity for microfluidic platform compatibility. Available pack sizes: 500 mL, 1000 mL.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration targets nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM is designed to reduce particulate clogging risk in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline and a phenol red–free formulation reduce optical background for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available pack sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate
Appearance Pale yellow-colored, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 35
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM High Glucose + NEAA + 25mM HEPES: 35 ingredients verified per lot with CAS numbers. NEAA components are included under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMGH-PBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water — reduces trace ionic and organic contaminants entering the formulation.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMGH-PBR1X compares

FluxMPS™ DCP-MEMGH-PBR1X vs. conventional 0.22 µm–filtered MEM High Glucose + NEAA formulations.

Parameter DCP-MEMGH-PBR1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm) Not specified Not specified
MEM High Glucose + NEAA + HEPES-only CO2-free — no sodium pyruvate, no sodium bicarbonate, no phenol red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMGH-PBR1X.

Yes. DCP-MEMGH-PBR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, targeting ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with mycoplasma-retentive filtration is well suited to primary neuronal, epithelial, and fibroblast OoC platforms.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — targeting approximately 5× fewer particles than a single-pass 0.22 µm filter, with mycoplasma-retentive filtration applied at every production run.
This formulation uses HEPES-only, CO2-independent buffering with no phenol red optical interference, leaving the secondary carbon source (sodium pyruvate) to be defined by the researcher. NEAA and L-Glutamine are already pre-loaded to reduce metabolic burden. Researchers requiring an additional carbon source can supplement sodium pyruvate directly; contact support@diagnocine.com for custom pre-formulated options.
No. This formulation is CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation, suitable for atmospheric incubation and open-bench handling.
Yes. Serum, growth factors and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never through a 0.04 µm membrane, which retains IgM, lipoproteins, and much of the functional serum fraction. Defined, protein-free additions may use 0.1 µm filtration.
Every batch is released against a specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, and a Certificate of Analysis is available for each lot on request. Low endotoxin is relevant to primary cell cultures because it limits TLR4-mediated fibroblast activation, neuronal inflammatory gene expression, and epithelial barrier disruption.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM High Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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