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FluxMPS™ RPMI Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-RPMIH-QPB1X
$44.00
DCP-RPMIH-QPB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (2.0 g/L) Without L-Glutamine Without Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-RPMIH-QPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic, lymphocyte, and immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms.[1,3] A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides bicarbonate-free, CO2-independent pH buffering. Formulation: [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

  • HEPES-buffered (25 mM, pKa 7.3 at 37°C), bicarbonate-free formulation for CO2-independent pH control during open-air handling and flow cytometry preparation
  • Glucose 2.0 g/L (2000 mg/L) as the sole defined carbon source; L-glutamine, sodium pyruvate, and sodium bicarbonate withheld so each can be titrated independently for metabolic flux studies
  • Reduced glutathione (1.0 mg/L) included for redox support in lymphocyte and hematopoietic cell culture
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — see Purity Architecture below
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Contains phenol red sodium salt (5.3 mg/L); a phenol-red-free variant is available on request for fluorescence-sensitive assays
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • 39 formulation components released with full raw-material CAS traceability; custom pH, salts, and nutrient modifications available on request
CAT. NO.
DCP-RPMIH-QPB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Formulation[+] Phenol Red, [+] HEPES, [+] Ca, [+] Mg, [+] Glucose | [-] Gln, [-] Pyruvate, [-] NaHCO3
  • Glucose2000 mg/L (2.0 g/L)
  • HEPES25 mM, pKa 7.3 at 37°C
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered RPMI passes subvisible particulates, mycoplasma in the 0.2–0.3 µm range, and endotoxin fragments that can confound sensitive lymphocyte and immune-cell assays. FluxMPS™ RPMI 1640 + 25mM HEPES is built to reduce these variables.[1]

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate compliance provide particulate-clean media for suspension immune cell OoC, flow cytometry, and lymphocyte perfusion chips.

target

Total metabolic control

L-glutamine, sodium pyruvate, and sodium bicarbonate are withheld so glucose (2.0 g/L), amino acids, and buffering can each be titrated independently for immune-cell metabolic flux and Warburg-effect studies under HEPES-buffered, CO2-independent conditions.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) feed stock supports low trace-metal and organic-carbon (TOC) background for sensitive lymphocyte and hematopoietic cell culture.

visibility

Low background for imaging

Quadruple-stage 0.04 µm filtration reduces particulate background for confocal imaging, biosensor, and flow cytometry channels. This formulation contains phenol red sodium salt (5.3 mg/L); a phenol-red-free variant is available on request for fluorescence-sensitive assays.

science

Rich, stable nutrient profile

RPMI 1640's amino acid and vitamin base, including reduced glutathione (1.0 mg/L) for redox support, is manufactured in micro-batches with full lot-to-lot CAS traceability across all 39 formulation components.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated 0.1 µm prefilter / 0.04 µm final-filter pairs — reach a 0.04 µm final cut-off prior to aseptic fill.[4]

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter, including the 0.2–0.3 µm mycoplasma size range.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

0.04
µm final pore size — sub-mycoplasma polishing
4
Filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled via 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every stage (not tested per lot; typical mycoplasma diameter 0.2–0.3 µm).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 with 25mM HEPES (DCP-RPMIH-QPB1X) quadruple-stage filtration system diagram showing 0.1 micron times 2 and 0.04 micron times 2 stages for organ-on-a-chip and microfluidic cell culture applications - Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-QPB1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-QPB1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable purity for hematopoietic cell and related OoC applications.[2,3]

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated perfusion and robotic culture systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off of this product
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. 0.04 µm filtration reduces particulate load that can confound immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based formulations would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip models on microphysiological system (MPS) platforms.[3]

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Glutamine-, pyruvate-, and bicarbonate-free RPMI base supports independently titrated glycolysis studies and ¹³C isotope tracing of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium; this formulation contains phenol red.

¹³C tracingGlycolysisLC-MS metabolomics
Live-Cell Imaging

Flow Cytometry & Confocal

Reduced particulate background from quadruple-stage 0.04 µm filtration supports flow cytometry and confocal imaging workflows; a phenol-red-free variant is available for fluorescence-sensitive channels.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
L-Glutamine None / Not added
Sodium Pyruvate None / Not added
Sodium Bicarbonate None / Not added
Phenol Red Present, 5.3 mg/L (phenol-red-free variant available on request)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H2O
Total ingredients 39 components across 4 categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — bicarbonate-free, 25 mM HEPES-buffered formulation
Available sizes 500 mL, 1000 mL
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 39 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others), verified per lot with CAS numbers for full raw-material traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
i-Inositol 87-89-8 35.000
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.00
Phenol red sodium salt 34487-61-1 5.300
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-QPB1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

filter_alt

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 filtration train reaching a 0.04 µm final cut-off prior to aseptic fill.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-QPB1X compares

FluxMPS™ DCP-RPMIH-QPB1X vs. conventional 0.22 µm-filtered RPMI formulations.

Parameter DCP-RPMIH-QPB1X (FluxMPS™) Conventional RPMI 1640 + 25mM HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Formulation — HEPES-only, no glutamine, no pyruvate, no bicarbonate check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 / 0.04 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes — Microfluidics Suitable (0.04 µm) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-QPB1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-QPB1X is processed through a quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, making it Microfluidics Suitable for immune-cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models on microphysiological system (MPS) platforms.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration.
This HEPES-only, bicarbonate-free formulation lets glutamine, pyruvate, and buffering be titrated independently of the base medium, giving maximum flexibility for immune-cell metabolic flux analysis in CO2-independent conditions. Add L-glutamine or a stable dipeptide substitute, sodium pyruvate, and/or sodium bicarbonate as required for your specific cell line and assay format. Contact support@diagnocine.com for a pre-supplemented custom formulation.
No. This formulation is bicarbonate-free and HEPES-buffered (25 mM, pKa 7.3 at 37°C), providing CO2-independent pH stability for open-air handling and non-CO2 incubation. Validate pH stability under your specific culture conditions and cell line.
Yes. FBS (typically 5–10%), serum-free supplements, growth factors, or antibiotics can be added as required. Filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane; use 0.1 µm only for defined, protein-free additions. Never filter serum through 0.04 µm — that pore size retains IgM, VLDL, chylomicrons, and much of the serum lipoprotein fraction. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. Low endotoxin levels reduce the risk of LPS-driven TLR4/NF-κB activation, which can confound lymphocyte proliferation and cytokine assays.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune-cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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