Luria-Bertani (LB) Agar Plates/W Penicllin G
Catalog.No: DCP-LBAPPG
Features:
Uses: Cloning (used to grow recominant E.coli that carry plasmids for cloning DNA), Microbial genetics, Classroom teachings
pH: pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 100µg/mL
Storage: Keep away from light at 2–8°C
Shipping Condition: Blue ice
Appearance: Yellow to light amber
Sterile
Shelf Life: 2 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Luria–Bertani (LB) agar with Penicillin G is a rich bacterial growth medium (standard LB agar) supplemented with a β-lactam antibiotic to inhibit susceptible bacteria; it is less common than LB with ampicillin for molecular cloning but is conceptually similar in how it works.
The LB base provides peptides, amino acids, vitamins, and salts that support robust growth of non-fastidious bacteria such as E. coli. Penicillin G is a β-lactam antibiotic that targets cell wall synthesis (binding penicillin-binding proteins and blocking peptidoglycan cross-linking), leading to lysis and death of actively growing, susceptible bacteria, especially many Gram-positives. Bacteria with appropriate β-lactam resistance mechanisms (e.g., β-lactamase production or altered PBPs) can grow and form colonies on LB-Pen G; susceptible cells are inhibited or killed. LB agar with Penicillin G can therefore be used as a selective medium to suppress penicillin-susceptible bacteria (commonly Gram-positives) when enriching for penicillin-resistant bacteria or focusing on Gram-negative flora.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
