Luria-Bertani (LB) Agar Plates/W Gentamicin
Catalog.No: DCP-LBAPG
Features:
Uses: Cloning (used to grow recominant E.coli that carry plasmids for cloning DNA), Microbial genetics, Classroom teachings
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 25µg/mL
Storage: Keep away from light at 2–8°C
Shipping Condition: Blue ice
Appearance: Yellow to light amber
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Luria–Bertani (LB) agar with gentamicin is a rich, non-selective bacterial growth medium (LB agar) that has been made selective by adding the aminoglycoside antibiotic gentamicin. It is used to grow and maintain bacteria that carry gentamicin-resistance genes (e.g., plasmid-encoded gentamicin resistance), while inhibiting non-resistant cells.
Gentamicin binds the 30S ribosomal subunit and inhibits protein synthesis; in susceptible bacteria, this leads to bactericidal activity. Bacteria that carry a gentamicin-resistance determinant (often a plasmid?encoded aminoglycoside?modifying enzyme) can grow and form colonies on LB agar with gentamicin. LB with gentamicin is a selective medium used for maintaining plasmids with gentamicin resistant markers, selecting transformants or transconjugants containing gentamicin-resistance cassettes, and routine cloning and genetic manipulation where gentamicin is the selectable marker.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
