Luria-Bertani (LB) Agar Plates/W Carbenicillin
Catalog.No: DCP-LBAPC
Features:
Uses: Cloning (used to grow recominant E.coli that carry plasmids for cloning DNA), Microbial genetics, Classroom teachings
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 100µg/mL
Storage: Keep away from light at 2–8°C
Shipping Condition: Blue ice
Appearance: Yellow to light amber
Sterile
Shelf life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Luria-Bertani (LB) agar with carbenicillin is a rich bacterial growth medium (standard LB agar) that has been made selective from the addition of β-lactam antibiotic carbenicillin, most commonly to grow and maintain plasmid-bearing, carbenicillin-resistant E.coli used in cloning and protein expression. The LB base provides peptides, amino acids, vitamins, and salts that support robust growth of non-fastidious bacteria such as the previously mentioned E.coli.
Carbenicillin is a β-lactam antibiotic that inhibits cell wall synthesis and can be bactericidal at effective concentrations. Ampicillin and carbenicillin are in a similar spectrum, with carbenicillin being more stable, which can lead to fewer satellite colonies being found. LB Agar with carbenicllin can be often seen being used for maintaining plasmids that encode β-lactam resistance in E. coli, and selecting transformants or recombinant clones carrying carbenicllin resistant plasmids in routine cloning and protein-expression workflows.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
