Luria-Bertani (LB) Agar Plates/W Ampicillin Sodium Salt
Catalog.No: DCP-LBAPAS
Features:
Uses: Cloning (used to grow recominant E.coli that carry plasmids for cloning DNA), Microbial genetics, Classroom teachings
pH: pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 50µg/mL
Storage: Keep away from light at 2–8°C
Shipping Condition: Blue ice
Appearance: Yellow to light amber
Sterile
Shelf life: 2 Weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Luria Bertani (LB) agar with ampicillin sodium salt is a rich bacterial growth medium rendered selective by adding ampicillin, used to grow and maintain baceria carrying an ampicilln-resistance gene (usually β-lactamase on a plasmid).
The LB base provides peptides, amino acids, vitamins and salts that support robust growth of non-fastidious bacteria. Ampicillin being a β-lactam antibiotic, inhibits cell wall synthesis and is a bactericidal for susceptible bacteria. Bacteria expressing a β-lactamase can grow and form colonies on LB-amp agar; non-resistant cells are inhibited or die. LB agar with ampicillin therefore is a selective medium widely used for maintaining plasmids with ampicillin resistant markers, selecting transformants or transconjugants carrying ampicillin-resistant cassettes, and routine cloning and genetic manipulation where ampicillin is the selectable marker.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
