FluxMPS™ Medium 199 w/ Earle's Salts: 2X Liquid
FluxMPS™ Medium 199 w/ Earle's Salts: 2X Liquid
FluxMPS™ DCP-M1992X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Medium 199 with Earle's Salts, supplied as a 2X liquid concentrate for 1:1 dilution to 1X working strength. Originally developed for primary chick embryo fibroblasts, it remains widely used for non-transformed primary cell maintenance and vaccine/virus production, and is engineered here for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Formulation: [+] Earle's Salts base, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Sodium Pyruvate, [+] Phenol Red.
- Medium 199 with Earle's Salts, supplied as a 2X concentrate — dilute 1:1 with sterile water or cell suspension to reach 1X working concentration
- Glucose supplied at 2000 mg/L (2.0 g/L) as the 2X concentrate, equivalent to 1000 mg/L (1X) after dilution
- Quadruple-stage filtration train: 0.1 µm prefilter → 0.04 µm final filter → 0.1 µm prefilter → 0.04 µm final polish
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Contains sodium bicarbonate (4400 mg/L as supplied) and phenol red sodium salt as a pH indicator — not a bicarbonate-free or phenol-red-free formulation
- Manufactured under an ISO 13485:2016 quality management system with Ultrapure Type 1 water (18.2 MΩ·cm) and ISO Class 5 aseptic fill
- 38 individually verified components across inorganic salts, amino acids, vitamins, and other supplements, each released with a Certificate of Analysis
- Custom formulation modifications (pH, concentration, additives) available on request — contact support@diagnocine.com
- Media familyMedium 199 with Earle's Salts (2X Liquid)
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
- Glucose2000 mg/L (2.0 g/L) as supplied [2X]; 1000 mg/L (1X) after 1:1 dilution
- AppearanceClear, red-colored solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered Medium 199 passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that confound sensitive cell culture applications. FluxMPS™ addresses these failure modes with a quadruple-stage filtration train reaching a 0.04 µm final cut-off.
Microchannel-safe purity
0.04 µm final filtration with USP <788> particulate release testing supports safe perfusion in microfluidic chip geometries.
Defined 2X concentrate control
Supplied at a fixed 2X concentration so the working (1X) nutrient and buffer profile is set precisely at the point of dilution, supporting reproducible metabolic conditions across replicate cultures.
Ultrapure-grade water
Formulated with Type 1 water (18.2 MΩ·cm), minimizing trace-metal and organic-carbon (TOC) contribution from the feed water used in manufacture.
Low background for imaging
Quadruple-stage 0.04 µm filtration keeps particulate baseline low for confocal microscopy and biosensor-based assays. This formulation contains phenol red and is not optimized for autofluorescence-sensitive imaging; a phenol-red-free variant is available on request.
Rich, stable nutrient profile
38 individually verified components — salts, amino acids, vitamins, glucose, sodium pyruvate — released per lot with a Certificate of Analysis.
Customization on demand
pH, concentration, component additions, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter/final-filter pairs — reach a final 0.04 µm polish. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter, giving the train full redundancy rather than a single descending cascade.
-
1
0.1 µm Prefiltration I
Removes large aggregates and cell debris; protects the first 0.04 µm final filter cartridge.
-
2
0.04 µm Final filtration I — Mycoplasma-retentive pass
Retains particulates and organisms in the 0.2–0.3 µm mycoplasma size range that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second, dedicated prefilter protecting the second 0.04 µm final filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter ahead of aseptic fill & finish in an ISO Class 5 (Class 100) environment.
Performance vs. conventional media
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
© Diagnocine® — DCP-M1992X
Specialized cell culture applications
FluxMPS™ DCP-M1992X — Medium 199 with Earle's Salts (2X) — is engineered for primary chick embryo fibroblasts and related cell models, with 0.04 µm filtration purity for OoC and microfluidic compatibility.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems and robotics platforms — a separate tier from the Microfluidics Suitable (0.04 µm) product on this page.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Organ-on-a-Chip & MPS Platforms
0.04 µm filtered Medium 199 helps prevent microchannel clogging in complex OoC platforms.
Virus Production & Vaccine Manufacturing
Medium 199 is widely used for flavivirus, herpesvirus, and vaccine virus production; 0.04 µm filtration supports a particle-controlled base medium.
Primary Cell Culture
Mycoplasma-retentive filtration and a < 0.05 EU/mL endotoxin release specification support sensitive primary cell models.
Non-Transformed Cell Maintenance
Historically formulated for maintenance of non-transformed cells and primary explants; supports serum-supplemented or serum-reduced workflows.
Metabolic Research & Isotope Tracing
Defined base composition supports ¹³C isotope tracing and LC-MS/GC-MS metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate baseline supports confocal microscopy and biosensor-based OoC applications; note this formulation contains phenol red.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate |
| Appearance | Clear, red-colored solution |
| Glucose | 2000 mg/L (2.0 g/L) as supplied [2X]; 1000 mg/L (1X) after 1:1 dilution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 38 (4 categories) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release spec — see §Manufacturing) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approx. 5–6% CO₂, derived from 2200 mg/L NaHCO₃ at 1X working concentration (target pH 7.4) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Medium 199 with Earle's Salts (2X): 38 ingredients verified per lot with CAS numbers, across inorganic salts, amino acids, vitamins, and other components. Supplied as a 2X concentrate — dilute 1:1 with sterile water or cell suspension to reach 1X working concentration.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 530.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Sodium bicarbonate | 144-55-8 | 4400.000 |
| Sodium chloride | 7647-14-5 | 13600.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 244.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Alanine | 56-41-7 | 17.800 |
| L-Arginine hydrochloride | 1119-34-2 | 252.000 |
| L-Asparagine monohydrate | 5794-13-8 | 30.000 |
| L-Aspartic acid | 56-84-8 | 26.600 |
| L-Cystine | 56-89-3 | 62.600 |
| L-Glutamic acid | 56-86-0 | 29.400 |
| L-Glutamine | 56-85-9 | 584.000 |
| Glycine | 56-40-6 | 15.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 84.000 |
| L-Hydroxyproline | 51-35-4 | 10.000 |
| L-Isoleucine | 73-32-5 | 104.000 |
| L-Leucine | 61-90-5 | 104.000 |
| L-Lysine hydrochloride | 657-27-2 | 145.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 64.000 |
| L-Proline | 147-85-3 | 23.000 |
| L-Serine | 56-45-1 | 21.000 |
| L-Threonine | 72-19-5 | 96.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 103.800 |
| L-Valine | 72-18-4 | 92.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 2.000 |
| D-Ca-Pantothenate | 137-08-6 | 2.000 |
| Folic acid | 59-30-3 | 2.000 |
| i-Inositol | 87-89-8 | 4.000 |
| Nicotinamide | 98-92-0 | 2.000 |
| Pyridoxal hydrochloride | 65-22-5 | 2.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Phenol red sodium salt | 34487-61-1 | 21.200 |
| Sodium pyruvate | 113-24-6 | 0.220 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feed water, minimizing trace-metal and TOC contribution.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — Certificate of Analysis available for every lot.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL, verified per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-M1992X compares
FluxMPS™ DCP-M1992X vs. conventional 0.22 µm–filtered Medium 199 and standard basal media.
| Parameter | DCP-M1992X (FluxMPS™) | Conventional Medium 199 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation (2X Earle's Salts base with L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate, Phenol Red) | check_circle Yes | cancel Varies by supplier | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle 0.1 µm retentive stage | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes (Method 1) | cancel Not specified | cancel Not specified |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel Limited | cancel Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-M1992X — Medium 199 with Earle's Salts (2X).
Supporting literature
Key publications supporting Medium 199 with Earle's Salts in cell culture and organ-on-a-chip applications.
- Morgan JF, Morton HJ, Parker RC. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc Soc Exp Biol Med. 1950;73:1–8. doi:10.3181/00379727-73-17557
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
