FluxMPS™ Medium 199 w/ Earle's Salts: 2X Liquid
FluxMPS™ Medium 199 w/ Earle's Salts: 2X Liquid
FluxMPS™ DCP-M1992X is an MPS-grade, ultra-filtered Medium 199 with Earle's Salts 2X formulation engineered for Primary chick embryo fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] Earle's Salts, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate. This is a 2X concentrated formulation — dilute 1:1 before use.
- Medium 199 with Earle's Salts 2X — Medium 199 is a 2X concentrated formulation
- Glucose: 1000 mg/L (1.0 g/L, 2X concentrated)
- 2X concentrated — dilute 1:1 with sterile water or cell suspension to reach 1X working concentration
- 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom formulation modifications available on request
- Media familyMedium 199 with Earle's Salts 2X
- Glucose2000 mg/L (2.0 g/L) [2X concentrated]
- Formulation[+] Earle's Salts, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate
- AppearanceClear, red-colored solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Shelf Life12 months / 2–8°C
Engineered where standard media fails
Conventional 0.22 µm–filtered Medium passes mycoplasma, subvisible particulates, and endotoxin fragments that confound sensitive cell culture applications. FluxMPS™ eliminates these failure modes with four-stage sub-0.04 µm filtration.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in microfluidic chip geometries.
Specialized formulation
Medium 199 is a 2X concentrated formulation. Contains nucleosides, coenzymes, cholesterol, and full amino acid/vitamin profile. Dilute 1:1 with sterile water or cell suspension to
Ultrapure-grade water
18.2 MΩ·cm — eliminates ionic contaminants affecting sensitive cell culture outcomes.
Below TLR4 endotoxin threshold
< 0.05 EU/mL endotoxin — prevents LPS-driven inflammatory activation confounding experimental results.
Rich nutrient profile
Medium delivers specialized nutrients for demanding cell types — particle-free for OoC use.
Customization on demand
pH, concentration, component additions, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates; protects downstream 0.04 µm membranes.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility; no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-M1992X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered media.
© Diagnocine® — DCP-M1992X
Specialized cell culture applications
FluxMPS™ DCP-M1992X — Medium 199 with Earle's Salts 2X — engineered for Primary chick embryo fibroblasts with 0.04 µm filtration purity for OoC compatibility.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available on request.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Organ-on-a-Chip & MPS
0.04 µm filtered Medium prevents microchannel clogging for complex OoC platforms.
Virus Production & Vaccine Manufacturing
Medium 199 is used for flavivirus, herpesvirus, and vaccine virus production — 0.04 µm filtration ensures particle-free base medium.
Primary Cell Culture
Sub-mycoplasma filtration and < 0.05 EU/mL endotoxin support sensitive primary cell models.
Metabolic Flux Analysis
Defined formulation base for ¹³C isotope tracing and Seahorse XF metabolic assays.
Microscopy & Optical Sensing
Ultra-low particulate; phenol red–free variants eliminate autofluorescence for confocal and biosensor OoC applications.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Earle's Salts, [+] L-Glutamine, [+] Sodium Bicarbonate |
| Appearance | Clear, red-colored solution |
| Glucose | 2000 mg/L (2.0 g/L) [2X concentrated] |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 60 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Medium 199 with Earle's Salts 2X: 38 ingredients verified per lot with CAS numbers. Medium 199 is a 2X concentrated formulation. Contains nucleosides, coenzymes, cholesterol, and full amino acid/vitamin profile. Dilute 1:1 with sterile water or cell suspension to reach 1X working concentration.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 530.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Sodium bicarbonate | 144-55-8 | 4400.000 |
| Sodium chloride | 7647-14-5 | 13600.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 244.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Alanine | 56-41-7 | 17.800 |
| L-Arginine hydrochloride | 1119-34-2 | 252.000 |
| L-Asparagine monohydrate | 5794-13-8 | 30.000 |
| L-Aspartic acid | 56-84-8 | 26.600 |
| L-Cystine | 56-89-3 | 62.600 |
| L-Glutamic acid | 56-86-0 | 29.400 |
| L-Glutamine | 56-85-9 | 584.000 |
| Glycine | 56-40-6 | 15.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 84.000 |
| L-Hydroxyproline | 51-35-4 | 10.000 |
| L-Isoleucine | 73-32-5 | 104.000 |
| L-Leucine | 61-90-5 | 104.000 |
| L-Lysine hydrochloride | 657-27-2 | 145.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 64.000 |
| L-Proline | 147-85-3 | 23.000 |
| L-Serine | 56-45-1 | 21.000 |
| L-Threonine | 72-19-5 | 96.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 103.800 |
| L-Valine | 72-18-4 | 92.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 2.000 |
| D-Ca-Pantothenate | 137-08-6 | 2.000 |
| Folic acid | 59-30-3 | 2.000 |
| i-Inositol | 87-89-8 | 4.000 |
| Nicotinamide | 98-92-0 | 2.000 |
| Pyridoxal hydrochloride | 65-22-5 | 2.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Phenol red sodium salt | 34487-61-1 | 21.200 |
| Sodium pyruvate | 113-24-6 | 0.220 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability for every lot on request.
How DCP-M1992X compares
FluxMPS™ DCP-M1992X vs. conventional 0.22 µm–filtered Medium formulations.
| Parameter | DCP-M1992X (FluxMPS™) | Conventional Medium 199 with (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Medium 199 2X with Earle's Salts — first defined synthetic medium; nucleosides, coenzymes, cholesterol; for virus production and primary epithelial OoC | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
Frequently asked questions
Common questions about FluxMPS™ DCP-M1992X — Medium 199 with Earle's Salts 2X.
Supporting literature
Key publications supporting Medium 199 with Earle's Salts 2X in cell culture and OoC applications.
- Morgan JF, Morton HJ, Parker RC. Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc Soc Exp Biol Med. 1950;73:1–8. doi:10.3181/00379727-73-17557
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
