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Human Glucokinase Regulatory Protein, GCKR ELISA Kit
Product#: BTL-E1470Hu
$686.40
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Human Glucokinase Regulatory Protein, GCKR ELISA Kit
Cat. No#: BTL-E1470Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.016ng/mL
Detection range: 0.05-15ng/mL
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
glucokinase regulatory protein
SKU#.: ? BTL- |
E1470Hu |
Assay type |
Sandwich |
Size |
0.016ng/mL |
Detection range |
0.05-15ng/mL |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
The glucokinase regulatory protein (GKRP) also known as glucokinase (hexokinase 4) regulator (GCKR) is a protein produced in hepatocytes (liver cells). GKRP binds and moves glucokinase (GK), thereby controlling both activity and intracellular location of this key enzyme of glucose metabolism. |
UniProt accession |
68,685 |
GeneID |
2646 |
Synonyms |
FGQTL5;GCKR;GKRP;Glucokinase regulator;Glucokinase regulatory protein |
Gene names |
GCKR |
Research area |
Others |
Target protein |
GCKR |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1470Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/mL | 2.413 | 2.504 | 2.446 |
| 2.595 | |||
| 4ng/mL | 1.597 | 1.582 | 1.523 |
| 1.566 | |||
| 2ng/mL | 0.9 | 0.81 | 0.751 |
| 0.72 | |||
| 1ng/mL | 0.378 | 0.396 | 0.337 |
| 0.414 | |||
| 0.5ng/mL | 0.247 | 0.255 | 0.196 |
| 0.262 | |||
| 0ng/mL | 0.052 | 0.058 | 0 |
| 0.064 |
Precision
We measured random samples of E1470Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.69 | 0.16 | 5.86 |
| Intra-Assay | 2 | 18 | 4.57 | 0.17 | 3.75 |
| Intra-Assay | 3 | 18 | 1.26 | 0.06 | 4.75 |