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Human Glucokinase, GCK ELISA Kit
Product#: BTL-E0773Hu
$686.40
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Human Glucokinase, GCK ELISA Kit
Cat. No#: BTL-E0773Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 4.85ng/L
Detection range: 10-2000ng/L
Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue
Reactive with: Human
Product overview
Full product name
Glucokinase
SKU#.: ? BTL- |
E0773Hu |
Assay type |
Sandwich |
Size |
4.85ng/L |
Detection range |
10-2000ng/L |
Sample type |
Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue |
Species |
Human |
Storage |
2-8°C |
Assay time |
1h 30m |
Background |
Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. |
UniProt accession |
52,191 |
GeneID |
2645 |
Synonyms |
GCK |
Gene names |
GCK |
Research area |
Signal Transduction;Cancer;Metabolism |
Target protein |
GCK |
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0773Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.179 | 2.208 | 2.162 |
| 2.237 | |||
| 600ng/L | 1.181 | 1.198 | 1.152 |
| 1.216 | |||
| 300ng/L | 0.821 | 0.821 | 0.775 |
| 0.821 | |||
| 150ng/L | 0.504 | 0.514 | 0.467 |
| 0.523 | |||
| 75ng/L | 0.26 | 0.227 | 0.181 |
| 0.194 | |||
| 0ng/L | 0.063 | 0.046 | 0 |
| 0.029 |
Precision
We measured random samples of E0773Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 263.3 | 19.3 | 7.3 |
| Intra-assay | 2 | 18 | 659.9 | 23.6 | 3.6 |
| Intra-assay | 3 | 18 | 426.7 | 24.1 | 5.6 |