DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
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Mouse Glucokinase (GK)
Cat.No.: JNB-JL13834
Size: 96TSpecies: MouseAssay Type: SandwichSensitivity: 0.05ng/mLDetection Range: 0.15-10ng/mLAlternative Names: Gck; GLK; HHF3; HK4; HKIV; HXKP; MODY2; Hexokinase-D; Hexokinase-4; Maturity Onset Diabetes Of The Young 2;Hexokinase type IVResearch Areas: MetabolismAssay Time: 2h45mAbbreviation: GKTest Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GK. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Mouse GK in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Mouse GK in the samples by comparing the OD of the samples to the standard curve.Specificity: This kit recognizes Mouse GK in samples. No significant cross-reactivity or interference between Mouse GK and analogues was observed.Recovery rate: 80%-120%Precision: Intra-CV and Inter-CV <10% Shipping: Ice Bag