Heptachlors (Heptachlor-cis-epoxide +Heptachlor) ELISA Kit
Product#: FNK-CB04-CD03
$891.71
Availability:
Ships in 1-2 Weeks
Heptachlors (Heptachlor-cis-epoxide +Heptachlor) ELISA Kit
Cat. No. FNK-CB04-CD03
Size 1 Kit
Storage 4°C
Description
The Heptachlors ELISA Kit is an indirect competitive ELISA (ic-ELISA). The assay is designed to detect and quantify the level of heptachlors (heptachlor-cis-epoxide +heptachlor) in soil.
Contents and sttorage
- Kit and components shipped at 4°C. Upon receipt, store the kit at 4°C.
- Stored at the enzyme labeled anti-dieldrin monoclonal antibody at -20°C.
Components
- Competitor coated 96 well stripe well plate (Competitor: Cyclodiene analogue conjugated with protein)
- Peroxidase labeled Anti-dieldrin monoclonal antibody
- Assay buffer
- Wash buffer
- Substrate
- Stop solution
- Plate seale
Customer-supplied materialComponents
- Standard compound (heptachlor-cis-epoxide)
Sensitivity
- Detection limit; 0.34 ng/mL, Quantification Limit; 1.10 ng/mL.
- The data were determined by JIS K0461:2006.
Intra-Assay Precision: 2.0- 3.0 % (n= 3)
Inter-Assay Precision: : 4.3- 4.6 % (n= 4)
Recovery (in soil samples): 86- 108 %
Note: The Competitive immunoassay of heptachlors was published as a guideline for quantification of heptachlor and its metabolites in polluted soils prior to squash planting. This guideline was established by the National Agriculture and Food Research Organization (NARO) at 2017.
Typical standard curve for heptachlor-cis-epoxide with indirect competitive ELIA (ic-ELISA)
Cross-reactivity
% of cross-reactivity was calculated at the 50 % binding point.
Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.
Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.
Correlation of heptachlors contents in soil between data of ic-ELISA and data of GC/MS
References
- Kataoka, C. et al. Development of a Model Immunoassay Utilizing Monoclonal Antibodies of Different Specificities for Quantitative Determination of Dieldrin and Heptachlors in Their Mixtures. J. Agr. and Food Chem., 2016, 64(46), 8950-8957.
- Kataoka, C. et al. Development of a Soil Sample-preparation Method for the Immunoassay of Heptachlors Residues before Cultivation (in Japanese), 2017, BUNSEKI KAGAKU, 66(10), 719-726.
