0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

FluxMPS™ Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-H12-B1X
$38.50
DCP-H12-B1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-H12-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered Ham's F-12 Nutrient Mixture engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. Processed through a validated filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm), it is formulated without sodium bicarbonate for CO2-independent culture and carries 1801.60 mg/L D-glucose, L-glutamine, and sodium pyruvate for robust energy metabolism.

  • High-glucose Ham's F-12 formulation (1801.60 mg/L D-Glucose) with L-glutamine (146.00 mg/L) and sodium pyruvate (110.10 mg/L) for robust energy metabolism
  • Sodium bicarbonate-free; formulated for CO2-independent culture — supplement with HEPES or another organic buffer for pH control
  • Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • 46 characterized components across inorganic salts, amino acids, vitamins, and other constituents — including zinc, putrescine, hypoxanthine, and thymidine typical of Ham's F-12
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, glucose, salts, and nutrient adjustments available on request
SKU: DCP-H12-B1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate: 1X Liquid
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (1801.60 mg/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)250-290 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Sterility (USP <71>)No growth / 14 days
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Total ingredients46 (4 categories)
  • Storage2-8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and microaggregates that clog microfluidic channels and interfere with sensitive assays. FluxMPS™ reduces these failure modes with a validated quadruple-stage filtration train reaching a 0.04 µm final cut-off.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in narrow chip geometries.

target

Total metabolic control

This formulation carries glucose, L-glutamine, and sodium pyruvate as defined energy substrates, with sodium bicarbonate deliberately excluded for CO2-independent buffering.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) with low trace-metal and organic carbon (TOC) content, supporting consistent formulation quality lot to lot.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and biosensor workflows. Note: this formulation contains phenol red (1.24 mg/L) — see Composition for full detail.

science

Rich, stable nutrient profile

46 ingredients verified per lot across inorganic salts, amino acids, vitamins, and other constituents; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and particulates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains mycoplasma-sized particles (0.2–0.3 µm) and sub-micron particulates not addressed by standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    Second, dedicated prefilter that protects the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-H12-B1X is filtered to a 0.04 µm final cut-off across four stages, delivering approximately 5× lower particulate counts by particle count versus standard 0.22 µm–filtered media.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.04 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-H12-B1X Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I mycoplasma-retentive barrier, 0.1 μm Prefiltration II, 0.04 μm Final filtration II polish ? Microfluidics Suitable cell culture media for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for microfluidic and organ-on-a-chip applications.
© Diagnocine® — DCP-H12-B1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-H12-B1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems and standard mammalian cell culture.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
  • Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Quadruple-stage 0.04 µm–filtered media reduces microchannel clogging risk in multi-organ chip architectures.

OoCToCBoCLoCMPS
Bioproduction

CHO & Mammalian Cell Culture

Formulated for CHO, cancer, primary, and clonal cell growth in serum-free or low-serum conditions.

CHOMCF-7HeLaHEK293
Stem Cell Biology

iPSC-Derived Models

Low endotoxin (< 0.05 EU/mL release specification) and mycoplasma-retentive filtration support sensitive iPSC protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Ultra-low particulate, endotoxin-controlled media for HUVEC monolayer integrity and TEER monitoring.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined glucose, glutamine, and pyruvate content supports 13C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline for confocal and biosensor platforms. This formulation contains phenol red (1.24 mg/L); phenol red-free variants are available on request.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (1801.60 mg/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> 250-290 mOsm/kg H2O
Glucose 1801.60 mg/L
L-Glutamine 146.00 mg/L
Sodium Pyruvate 110.10 mg/L
Phenol Red 1.24 mg/L
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification; see §Quality Assurance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1 NMT 25/mL
Particulate ≥25 µm USP <788> M1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent; supplement with HEPES (10-25 mM) or another organic buffer for pH control
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

46 ingredients verified per lot with CAS numbers for raw-material traceability. Ham's F-12 contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 44.100
Copper sulfate pentahydrate 7758-99-8 0.0025
Ferrous sulfate heptahydrate 7782-63-0 0.834
Magnesium chloride anhydrous 7786-30-3 57.650
Potassium chloride 7447-40-7 223.600
Sodium chloride 7647-14-5 7599.000
Sodium phosphate dibasic anhydrous 7558-79-4 142.040
Zinc sulfate heptahydrate 7446-20-0 0.863
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 210.700
L-Asparagine monohydrate 5794-13-8 15.010
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 35.120
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 146.00
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Isoleucine 73-32-5 3.940
L-Leucine 61-90-5 13.100
L-Lysine hydrochloride 657-27-2 36.500
L-Methionine 63-68-3 4.480
L-Phenylalanine 63-91-2 4.960
L-Proline 147-85-3 34.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 11.900
L-Tryptophan 73-22-3 2.040
L-Tyrosine disodium salt dihydrate 69847-15-0 7.810
L-Valine 72-18-4 11.700
Component CAS Number mg/L
VITAMINS
Biotin 58-85-5 0.0073
Choline chloride 67-48-1 13.960
D-Ca-Pantothenate 137-08-6 0.480
Folic acid 59-30-3 1.320
Nicotinamide 98-92-0 0.037
Pyridoxal hydrochloride 65-22-5 0.062
Riboflavin 83-88-5 0.038
Thiamine hydrochloride 67-03-8 0.340
Vitamin B12 68-19-9 1.360
i-Inositol 87-89-8 18.000
OTHERS
D-Glucose 50-99-7 1801.600
Hypoxanthine Sodium Salt 45738-97-4 4.770
Linoleic acid 60-33-3 0.084
Lipoic acid 1077-28-7 0.210
Phenol red Sodium Salt 34487-61-1 1.240
Putrescine dihydrochloride 333-93-7 0.161
Sodium pyruvate 113-24-6 110.100
Thymidine 50-89-5 0.730
Custom formulation: Contact support@diagnocine.com for DCP-H12-B1X custom specifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity, with low trace-metal and organic carbon (TOC) content for consistent formulation quality.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL, batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 250-290 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-H12-B1X compares

FluxMPS™ DCP-H12-B1X vs. conventional 0.22 µm–filtered Ham's F-12 formulations.

Parameter DCP-H12-B1X (FluxMPS™) Conventional Ham's F-12
(0.22 µm filtered)		 Standard Alt.
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
Ham's F-12 without Sodium Bicarbonate — CO2-independent with HEPES supplementation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-H12-B1X.

Yes. DCP-H12-B1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, tissue-on-a-chip (ToC), and lab-on-a-chip (LoC) platforms. It is Microfluidics Suitable at this 0.04 µm grade.
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
Sodium bicarbonate is excluded so the medium can be used in CO2-independent culture systems. Supplement with HEPES (10-25 mM) for open-top chip or ambient-atmosphere systems, or add sodium bicarbonate separately if a standard CO2 incubator buffering system is preferred.
No. This formulation is CO2-independent because sodium bicarbonate is excluded. Use HEPES or another organic buffer for pH control in ambient air.
Yes. Serum and protein-containing supplements (e.g., FBS, typically 5-10%) should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is not suitable for serum and will clog. Defined, protein-free additions may use a 0.1 µm filter. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis reporting the batch result is available on request.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288-293. doi:10.1073/pnas.53.2.288
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  7. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j
  8. Kilkenny C, et al. Guidelines for reporting animal and cell-based research (ARRIVE). PLoS Biol. 2010;8:e1000412. doi:10.1371/journal.pbio.1000412

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X