FluxMPS™ Ham's F-12 Nutrient Mixture w/o Phenol Red, Folic Acid: 1X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture w/o Phenol Red, Folic Acid: 1X Liquid
FluxMPS™ DCP-H12-RF1X is a Microfluidics Suitable, ultra-filtered Ham's F-12 formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulated without Phenol Red and Folic Acid for autofluorescence-sensitive imaging and DHFR-based selection workflows.
- Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
- Endotoxin specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Formulation: [+] Glucose (1801.60 mg/L), [+] L-Glutamine (146.00 mg/L), [+] Sodium Pyruvate (110.10 mg/L), [+] Sodium Bicarbonate (1176.00 mg/L) | [-] Phenol Red, [-] Folic Acid
- Phenol red–free and folic acid–free formulation supports low-background imaging and DHFR selection in CHO cell-line development
- Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 (Class 100) aseptic fill
- Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and TOC content
- Custom pH, salts, and nutrient adjustments available on request
- Formulation[+] Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium | [-] Phenol Red
- AppearanceOrange-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)280 - 320 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Total ingredients45
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration.
Microchannel-safe purity
0.04 µm final filtration and USP <788> Method 1 particulate compliance support safe perfusion in fine microfluidic chip geometries.
Total metabolic control
Defined inclusion of glutamine, pyruvate, and sodium bicarbonate delivers precise nutrient and buffer control for your protocol.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content for consistent formulation quality.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and biosensor platforms requiring minimal background interference.
Rich, stable nutrient profile
45 ingredients verified per lot; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma-scale purity for microfluidic channel geometries.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris and protein precipitates while providing mycoplasma-retentive filtration (mycoplasma diameter 0.2–0.3 µm); protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge; redundant pass with no breakthrough from Stage 1.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-H12-RF1X
Designed for next-generation cell models
FluxMPS™ DCP-H12-RF1X supports demanding platforms from single-channel microfluidic chips to CHO-based bioproduction workflows.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Ultra-clean 0.04 µm–filtered media helps prevent microchannel clogging in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Folic acid-free formulation supports DHFR-based selection systems for CHO cell-line development, alongside general mammalian and clonal culture.
iPSC-Derived Models
Ultra-low endotoxin specification (< 0.05 EU/mL) and mycoplasma-retentive filtration for sensitive iPSC protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled media for HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined formulation provides a clean nutrient background for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline for confocal and biosensor platforms; formulated without phenol red to reduce background fluorescence.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium | [-] Phenol Red |
| Appearance | Orange-colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 280 - 320 mOsm/kg H2O |
| Glucose | 1801.60 mg/L |
| L-Glutamine | 146.00 mg/L |
| Sodium Pyruvate | 110.10 mg/L |
| Phenol Red | Not added |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (per manufacturing batch) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | 5% CO2 required (sodium bicarbonate-containing formulation) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
45 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-12 contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 44.100 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride anhydrous | 7786-30-3 | 57.650 |
| Potassium chloride | 7447-40-7 | 223.600 |
| Sodium bicarbonate | 144-55-8 | 1176.000 |
| Sodium chloride | 7647-14-5 | 7599.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 142.040 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.863 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 210.700 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 146.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Isoleucine | 73-32-5 | 3.940 |
| L-Leucine | 61-90-5 | 13.100 |
| L-Lysine hydrochloride | 657-27-2 | 36.500 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 34.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 11.900 |
| L-Tryptophan | 73-22-3 | 2.040 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 7.810 |
| L-Valine | 72-18-4 | 11.700 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Nicotinamide | 98-92-0 | 0.037 |
| Pyridoxal hydrochloride | 65-22-5 | 0.062 |
| Riboflavin | 83-88-5 | 0.038 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1801.600 |
| Hypoxanthine Sodium Salt | 45738-97-4 | 4.770 |
| i-Inositol | 87-89-8 | 18.000 |
| Linoleic acid | 60-33-3 | 0.084 |
| Lipoic acid | 1077-28-7 | 0.210 |
| Putrescine dihydrochloride | 333-93-7 | 0.161 |
| Sodium pyruvate | 113-24-6 | 110.100 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with controlled trace-metal and TOC content for consistent, reproducible formulations.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every batch.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 280 - 320 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every batch on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-H12-RF1X compares
FluxMPS™ DCP-H12-RF1X vs. conventional 0.22 µm–filtered Ham's F-12 formulations.
| Parameter | DCP-H12-RF1X (FluxMPS™) | Conventional Ham's F-12 (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Formulation | [+] Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium | [-] Phenol Red | Standard Ham's F-12 (phenol red present) | Standard Ham's F-12 (phenol red present) |
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Ham's F-12 without Phenol Red and Folic Acid — imaging-clean DHFR selection base | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes (Method 1) | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-H12-RF1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j