FluxMPS™ Ham's F-12 Nutrient Mixture w/o Calcium Chloride: 1X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture w/o Calcium Chloride: 1X Liquid
FluxMPS™ DCP-H12-C1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Ham's F-12 formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Magnesium, [+] 1801.60 mg/L Glucose, [+] Sodium Pyruvate | [-] Calcium Chloride.
- Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for microchannel-safe purity
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Formulated with 1801.60 mg/L D-Glucose, 146 mg/L L-Glutamine, 110.10 mg/L Sodium Pyruvate, and 1176 mg/L Sodium Bicarbonate; Calcium Chloride not added
- 45 verified ingredients across inorganic salts, amino acids, vitamins, and other components, released per lot
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Calcium-free formulation supports enzymatic cell dissociation, calcium-imaging studies, and tight-junction permeability protocols
- Custom pH, salts, and nutrient adjustments available on request — contact support@diagnocine.com
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Magnesium, [+] 1801.60 mg/L Glucose, [+] Sodium Pyruvate | [-] Calcium Chloride
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Total ingredients45 across 4 groups
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and aggregate debris that clog microfluidic channels and interfere with sensor signals. FluxMPS™ is built on four-stage sub-0.04 µm filtration to address these failure modes.
Microchannel-safe purity
0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate compliance support safe perfusion across a range of microchannel geometries.
Total metabolic control
Selective inclusion/exclusion of glutamine, pyruvate, bicarbonate, and calcium chloride for precise nutrient and buffer definition per protocol.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon content for consistent raw-material quality lot to lot.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and biosensor applications; this formulation contains phenol red, so optical background should be evaluated per protocol.
Rich, stable nutrient profile
45 ingredients verified per lot across inorganic salts, amino acids, vitamins, and other components; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reach a final 0.04 µm polish, delivering sub-mycoplasma-sized particulate reduction not achieved by single-stage 0.22 µm filtration.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and protein particulates; protects the first 0.04 µm final filter cartridge.
-
2
0.04 µm Final filtration I
Retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
A second dedicated prefilter protects the second 0.04 µm final-filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter under ISO Class 5 (Class 100) aseptic fill & finish conditions.
Performance vs. conventional media
© Diagnocine® — DCP-H12-C1X
Designed for next-generation cell models
FluxMPS™ DCP-H12-C1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems, and calcium-free protocols requiring precise extracellular calcium control.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling risk on solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
0.04 µm–filtered media reduces microchannel clogging risk in complex multi-organ chip architectures.
Calcium-Free Dissociation Protocols
Calcium chloride is not added, supporting enzymatic cell dissociation and protocols requiring calcium-free extracellular conditions.
iPSC-Derived Models
Low endotoxin release specification (< 0.05 EU/mL) supports sensitive iPSC differentiation and maintenance protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled media supports HUVEC monolayer integrity and calcium-signaling studies.
Metabolic Flux Analysis
Defined formulation supports ¹³C isotope tracing and NMR metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate baseline supports confocal microscopy and biosensor platforms; contains phenol red, so background fluorescence should be assessed per application.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine (146 mg/L), [+] Sodium Bicarbonate (1176 mg/L), [+] Phenol Red (1.24 mg/L), [+] Magnesium (57.65 mg/L MgCl2), [+] Glucose (1801.60 mg/L), [+] Sodium Pyruvate (110.10 mg/L) | [-] Calcium Chloride (not added) |
| Appearance | Red-colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 45 across 4 groups |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ recommended (bicarbonate-buffered formulation, 1176 mg/L NaHCO3) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
45 ingredients verified per lot with CAS numbers for raw-material traceability. Ham's F-12 without Calcium Chloride contains zinc, putrescine, hypoxanthine, thymidine, and linoleic/lipoic acids in addition to standard amino acids and vitamins.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride anhydrous | 7786-30-3 | 57.650 |
| Potassium chloride | 7447-40-7 | 223.600 |
| Sodium bicarbonate | 144-55-8 | 1176.000 |
| Sodium chloride | 7647-14-5 | 7599.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 142.040 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.863 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 210.700 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine hydrochloride monohydrate | 30925-07-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 146.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Isoleucine | 73-32-5 | 3.940 |
| L-Leucine | 61-90-5 | 13.100 |
| L-Lysine hydrochloride | 657-27-2 | 36.500 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 34.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 11.900 |
| L-Tryptophan | 73-22-3 | 2.040 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 7.810 |
| L-Valine | 72-18-4 | 11.700 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Nicotinamide | 98-92-0 | 0.037 |
| Pyridoxal hydrochloride | 65-22-5 | 0.062 |
| Riboflavin | 83-88-5 | 0.038 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 18.000 |
| D-Glucose | 50-99-7 | 1801.600 |
| Hypoxanthine Sodium Salt | 45738-97-4 | 4.770 |
| Linoleic acid | 60-33-3 | 0.084 |
| Lipoic acid | 1077-28-7 | 0.210 |
| Phenol red Sodium Salt | 34487-61-1 | 1.240 |
| Putrescine dihydrochloride | 333-93-7 | 0.161 |
| Sodium pyruvate | 113-24-6 | 110.100 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with controlled trace-metal and organic-carbon content for consistent raw-material quality.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production with full per-lot traceability and a Certificate of Analysis for every batch.
Endotoxin — USP <85> BET
LAL assay; per-batch release specification < 0.05 EU/mL (assay sensitivity 0.005 EU/mL).
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every batch on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-H12-C1X compares
FluxMPS™ DCP-H12-C1X vs. conventional 0.22 µm–filtered Ham's F-12 formulations.
| Parameter | DCP-H12-C1X (FluxMPS™) | Conventional Ham's F-12 (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Ham's F-12 without Calcium Chloride — calcium-free for dissociation and signaling control | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Yes — Microfluidics Suitable | cancel Higher clogging risk | cancel Higher clogging risk |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-H12-C1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Brieva TA, Moghe PV. Engineering the calcium dynamics of cortical neural networks for prosthesis development. Biotechnol Bioeng. 2004;85:590–600. doi:10.1002/bit.10832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j