What makes the filtration process superior to standard 0.22 micron filtered media?

FluxMPS uses four sequential filtration passes - 0.1 micron prefiltration I, 0.04 micron final filtration I, 0.1 micron prefiltration II, and 0.04 micron final filtration II (polish) - each 0.04 micron final filter protected by its own dedicated 0.1 micron prefilter, resulting in approximately 5 times lower particulate counts than a single 0.22 micron pass.

FluxMPS RPMI 1640 Medium w/o L-Glutamine, Phenol Red: 1X Liquid

Q: Can DCP-RPMI-QR1X be supplemented with serum, growth factors, or other components?

Yes. FBS (typically 5-10 percent), growth factors, cytokines, or antibiotics may be added. Filter serum-containing or protein-containing supplements through a 0.2 micron low-protein-binding PES or PVDF membrane rather than a 0.04 micron membrane, which retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.

Q: What endotoxin level should I expect, and how is it verified?

The release specification is less than 0.05 EU/mL by LAL assay (USP less than 85 greater than, assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification prior to shipment. A Certificate of Analysis reporting the batch result is available on request.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. Full CoA per lot covers: appearance, pH (USP less than 791 greater than), osmolality (USP less than 785 greater than), sterility (USP less than 71 greater than), endotoxin (USP less than 85 greater than), mycoplasma control, particulate count (USP less than 788 greater than Method 1), and raw-material traceability. Request at support@diagnocine.com.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Phenol Red — 1X Liquid

Sodium Bicarbonate Calcium Magnesium 2.0 g/L Glucose Sodium Pyruvate L-Glutamine Phenol Red

FluxMPS™ DCP-RPMI-QR1X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation engineered for hematopoietic cells, lymphocytes, and immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

High glucose formulation (2.0 g/L) with sodium pyruvate (110 mg/L) supporting hematopoietic and lymphocyte energetics
Glutathione (reduced, 1.0 mg/L) included — antioxidant support for redox-sensitive immune cell assays
Formulated without L-glutamine and without phenol red for precise nitrogen control and dye-free imaging/flow cytometry
Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — Microfluidics Suitable for OoC and MPS channel geometries
Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
Manufactured under an ISO 13485:2016 quality management system with full lot traceability
39 individually verified components across three composition categories, with CAS numbers for raw-material traceability
Custom pH, nutrient, and salt modifications available on request — contact support@diagnocine.com

CAT. NO.

DCP-RPMI-QR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 w/o L-Glutamine, Phenol Red — 1X Liquid

Media familyRPMI 1640
Formulation[+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red
Glucose2000 mg/L (2.0 g/L)
Sodium Pyruvate110 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)280 - 320 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized particulates, subvisible aggregates, and endotoxin fragments that can activate TLR4 signaling and confound immune cell assays. FluxMPS™ is built around a finer filtration train and a documented specification matrix.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Low-particulate media suited to suspension immune cell OoC and flow cytometry workflows.

science

Immune cell–optimized formulation

RPMI 1640 contains reduced glutathione and a balanced amino acid/vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Trace-metal & TOC controlled water

Type 1 water (18.2 MΩ·cm) processed for low trace-metal and organic-carbon content, reducing lot-to-lot chemical background in sensitive immune assays.

visibility

Low background for imaging

Confocal and flow cytometry workflows benefit from the low particulate baseline delivered by 0.04 µm final filtration and the phenol red–free formulation.

shield

Batch-controlled endotoxin

< 0.05 EU/mL release specification (LAL, USP <85>), tested per manufacturing batch to reduce the risk of TLR4-mediated immune cell activation artefacts.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

1

0.1 µm Prefiltration I

Removes large aggregates and cell debris; protects the first 0.04 µm final-filter cartridge.
2

0.04 µm Final filtration I

Retains sub-micron particulates and organisms in the mycoplasma size range (0.2–0.3 µm) that pass a standard 0.22 µm filter.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filtration ahead of aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-RPMI-QR1X is processed through two dedicated prefilter/final-filter pairs run in series, versus the single 0.22 µm pass used in conventional RPMI, resulting in approximately 5× lower particulate counts by particle count.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm final pore size across 4 filtration passes

Sterility & mycoplasma control: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS Quadruple-stage filtration system diagram for FluxMPS RPMI 1640 Medium w/o L-Glutamine, Phenol Red (DCP-RPMI-QR1X) - 0.1 micron x2 plus 0.04 micron x2 filtration for organ-on-a-chip and microfluidic cell culture - Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-QR1X

Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-QR1X — RPMI 1640 without L-glutamine, without phenol red — delivers 0.04 µm filtered purity for hematopoietic cells and related microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems.

Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base medium for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration reduces particulate-driven activation artefacts in immune assays.

T cellsB cellsNK cellsPBMC

Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports hematopoietic cancer lines such as Jurkat, Raji, K562, and HL-60, where DMEM-based media would alter proliferation and signaling behavior.

JurkatRajiK562HL-60

Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where a low-particulate medium is required.

Tumor-immune chipLymph node OoCMPS

Immunotherapy

CAR-T & TIL Expansion

The < 0.05 EU/mL endotoxin release specification supports CAR-T manufacturing and TIL expansion protocols where endotoxin control is critical.

CAR-TTILTCR-T

Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base supports glycolysis stress-test protocols and ¹³C isotope tracing of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingGlycolysis

Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate, phenol red-free formulation supports PE-channel flow cytometry and confocal imaging without dye interference.

Flow cytometryConfocalELISA

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose, [+] Sodium Pyruvate \| [-] L-Glutamine, [-] Phenol Red
Appearance	Colorless to pale yellow, clear solution
Glucose	2000 mg/L (2.0 g/L)
pH USP <791>	7.4
Osmolality USP <785>	280 - 320 mOsm/kg H₂O
Total ingredients	39

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ required (sodium bicarbonate buffering)

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640: 39 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains reduced glutathione and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2000.000
Sodium chloride	7647-14-5	6000.00
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
i-Inositol	87-89-8	35.000
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	2000.000
Glutathione reduced	70-18-8	1.000
Sodium Pyruvate	113-24-6	110.000

Custom formulation: Contact support@diagnocine.com for DCP-RPMI-QR1X modifications.

Available pack sizes: 500 mL, 1000 mL.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured by ISO 13485-certified suppliers. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, low trace-metal and organic-carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, controlled per manufacturing batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 280 - 320 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-RPMI-QR1X compares

FluxMPS™ DCP-RPMI-QR1X vs. published specifications for conventional 0.22 µm–filtered RPMI/DMEM media.

Parameter	DCP-RPMI-QR1X (FluxMPS™)	Comparison
Grade	Microfluidics Suitable	Not specified (conventional 0.22 µm media)
Formulation trait	RPMI 1640, w/o L-Glutamine, w/o Phenol Red	Standard RPMI 1640 (contains L-Glutamine, Phenol Red)
Final filtration pore size	0.04 µm	0.22 µm
Number of filtration stages	4 (Quadruple-stage)	1
Mycoplasma-retentive filtration	check_circle Yes (0.1/0.04 µm)	cancel No
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested	check_circle Yes	Not specified
Water quality	Type 1, 18.2 MΩ·cm	Not specified
Manufacturing QMS	ISO 13485:2016	Not specified
Microfluidic channel compatibility	check_circle Yes	cancel Elevated clogging risk
Custom formulation	check_circle Yes	Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-QR1X — RPMI 1640 w/o L-Glutamine, Phenol Red.

Is DCP-RPMI-QR1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMI-QR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, and is Microfluidics Suitable for immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models where standard RPMI can carry particulate loads that trigger TLR4 activation.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

FluxMPS™ uses four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — each 0.04 µm final filter protected by its own dedicated 0.1 µm prefilter, resulting in approximately 5× lower particulate counts than a single 0.22 µm pass.

Why are L-glutamine and phenol red both excluded from DCP-RPMI-QR1X, and how do I supplement it?expand_more

Phenol red is omitted for dye-free flow cytometry and confocal imaging; L-glutamine is omitted so it can be added fresh at the time of use, since glutamine degrades in liquid storage. Add L-glutamine (or a stabilized dipeptide substitute) to your target concentration immediately before use.

Does this medium require a CO₂ incubator?expand_more

Yes. This formulation contains sodium bicarbonate (2000 mg/L) as its buffering system and requires a 5% CO₂ incubator atmosphere to maintain pH 7.4.

Can DCP-RPMI-QR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. FBS (typically 5–10%), growth factors, cytokines, or antibiotics may be added. Filter serum-containing or protein-containing supplements through a 0.2 µm low-protein-binding PES or PVDF membrane rather than a 0.04 µm membrane, which retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.

What endotoxin level should I expect, and how is it verified?expand_more

The release specification is < 0.05 EU/mL by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification prior to shipment. A Certificate of Analysis reporting the batch result is available on request.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and organ-on-a-chip applications.

Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Ronan JL, et al. From neural development to cognition: unexpected roles for chromatin. Nat Rev Genet. 2013;14:347–359. Cited for lymphocyte differentiation media context. doi:10.1038/nrg3413
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
Novak R, et al. Scalable fluidic platform for multiplexed organ-on-chip physiology. Nat Biomed Eng. 2020;4:407–420. Cited for immune-competent chip perfusion context. doi:10.1038/s41551-019-0497-x

FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Phenol Red: 1X Liquid