FluxMPS™ Ham's F-10 Nutrient Mixture with 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Ham's F-10 Nutrient Mixture with 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-H10H-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered Ham's F-10 + 25mM HEPES formulation engineered for CHO cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides CO₂-independent pH buffering in this bicarbonate-free formulation.
- Glucose source: 1100 mg/L (1.1 g/L) D-Glucose supporting CHO clonal growth
- 25 mM HEPES (pKa 7.3 at 37°C) — pH buffering independent of CO₂ supplementation
- Bicarbonate-free formulation designed for atmospheric incubators, open-top chip systems, and automated bioreactors
- Trace nutrients — zinc, hypoxanthine, thymidine, lipoic acid — supporting CHO and primary fibroblast clonal growth
- Four-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II
- Endotoxin release specification < 0.05 EU/mL (USP <85>, LAL assay)
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa NJ
- Glucose1100 mg/L (1.1 g/L)
- L-Glutamine146 mg/L
- Sodium Pyruvate110 mg/L
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered Ham's F-10 passes subvisible particulates and microaggregates that clog microfluidic channels and interfere with sensitive perfusion systems. FluxMPS™ is built to address these failure modes.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports perfusion in narrow chip geometries.
Cell-type–optimized formulation
Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid supporting CHO cell and primary fibroblast clonal growth.
Ultrapure-grade water
18.2 MΩ·cm Type 1 water with low trace-metal and organic-carbon (TOC) content supports reproducible formulation control.
Batch-controlled endotoxin
Formulated to a < 0.05 EU/mL release specification (USP <85>), relevant for primary cell assays sensitive to TLR4-mediated endotoxin responses.
HEPES pH stability
HEPES (25 mM) buffers pH during open-air handling, flow cytometry preparation, and atmospheric incubation without CO₂ supplementation.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.
-
1
0.1 µm Prefiltration I
Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge in this paired train.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter prior to aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-H10H-B1X
Specialized cell culture applications
FluxMPS™ DCP-H10H-B1X — Ham's F-10 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable purity for CHO cells and related OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotics platforms.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
CHO & Mammalian Cell Culture
Optimized for CHO, hybridoma, myeloma, and primary fibroblast culture in serum-free or low-serum conditions.
Organ-on-a-Chip & MPS
0.04 µm filtered media supports low-clog perfusion in complex multi-organ chip architectures.
Cancer Cell Lines
Supports fibroblast co-culture tumor models and primary cancer cell culture in the defined nutrient environment of Ham's F-10.
Primary & Stem Cell Support
Rich nutrient profile with trace elements supports primary cell viability and stem cell maintenance in low-serum conditions.
Metabolic Flux Analysis
Defined nutrient base for ¹³C isotope tracing and NMR metabolomics in specialized cell culture models. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.
Microscopy & Optical Sensing
Ultra-low particulate baseline supports confocal microscopy and biosensor applications. This formulation contains phenol red (1.3 mg/L) — see Composition for phenol-red-free alternatives.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (1100 mg/L), [+] Sodium Pyruvate (110 mg/L) | [-] Sodium Bicarbonate |
| Appearance | Orange-Yellow colored, clear solution |
| Glucose | 1100 mg/L (1.1 g/L) |
| HEPES | 25 mM (pKa 7.3 at 37°C) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 46 components across 4 categories |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent — HEPES alone maintains pH without gas supplementation |
| Available pack sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Ham's F-10 + 25mM HEPES: 46 ingredients verified per lot with CAS numbers for raw-material traceability. Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid supporting CHO cell and primary fibroblast clonal growth.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 44.100 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride anhydrous | 7786-30-3 | 74.640 |
| Potassium chloride | 7447-40-7 | 285.000 |
| Potassium phosphate monobasic | 7778-77-0 | 83.000 |
| Sodium chloride | 7647-14-5 | 7400.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 153.700 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.0288 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 211.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 146.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 21.000 |
| L-Isoleucine | 73-32-5 | 2.600 |
| L-Leucine | 61-90-5 | 13.100 |
| L-Lysine hydrochloride | 657-27-2 | 29.300 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 3.570 |
| L-Tryptophan | 73-22-3 | 0.600 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 2.610 |
| L-Valine | 72-18-4 | 3.500 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.024 |
| Choline chloride | 67-48-1 | 0.698 |
| D-Ca-Pantothenate | 137-08-6 | 0.715 |
| Folic acid | 59-30-3 | 1.320 |
| Nicotinamide | 98-92-0 | 0.615 |
| Pyridoxal hydrochloride | 65-22-5 | 0.206 |
| Riboflavin | 83-88-5 | 0.376 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 1.360 |
| i-Inositol | 87-89-8 | 0.541 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1100.000 |
| HEPES buffer | 7365-45-9 | 5958.000 |
| Hypoxanthine Sodium Salt | 5765-44-6 | 4.080 |
| Lipoic acid | 1077-28-7 | 0.210 |
| Phenol red Sodium Salt | 143-74-8 | 1.300 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm Type 1 water; low trace-metal and organic-carbon (TOC) content supports reproducible formulation control.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration count: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-H10H-B1X compares
FluxMPS™ DCP-H10H-B1X vs. conventional 0.22 µm–filtered Ham's formulations.
| Parameter | DCP-H10H-B1X (FluxMPS™) | Conventional Ham's F-10 + 25 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| Ham's F-10 + HEPES-only CO₂-free buffering — no sodium bicarbonate, for atmospheric CHO and fibroblast culture | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle 0.1 µm (not tested per lot) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-H10H-B1X — Ham's F-10 + 25mM HEPES.
Supporting literature
Key publications supporting Ham's F-10 + 25mM HEPES in specialized cell culture and OoC applications.
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
