TBS Blocking Buffer with Gelatin (1%)
FluxMPS™ TBS Blocking Buffer with Gelatin (1%)
An MPS-grade, sterile Tris-buffered saline blocking reagent formulated with 1% gelatin, engineered for a stable pH of 7.4 and defined 50 mM Tris-Base / 150 mM Sodium Chloride molarity. Purified through a quadruple-stage 0.1 µm and 0.04 µm membrane filtration architecture, it reduces non-specific binding and background noise across Western blotting, ELISA, and immunohistochemistry workflows, including organ-on-a-chip and microfluidic assay formats.
- Quadruple-stage filtration: 0.1 µm membrane filtered twice and 0.04 µm membrane filtered twice for ultra-low particulate purity
- Sterile, ultrapure Tris-buffered saline base with 1% gelatin blocking agent
- Stable target pH of 7.4 for consistent immunoassay performance
- DNase- and RNase-free, verified by direct incubation testing
- Compatible with nitrocellulose, nylon, and charge-modified nylon membranes
- Manufactured under ISO 13485-certified, CE-approved facilities
- Customizable concentration, pH, and additive formulation on request
- pH7.4
- Tris-Base Concentration50 mM
- Sodium Chloride150 mM
- Gelatin1%
- Filtration0.1 µm (2x) + 0.04 µm (2x)
- DNase ActivityNone Detected
- RNase ActivityNone Detected
- Storage4°C (-20°C long-term)
- Shelf Life6 months
- FormatSterile Liquid Buffer
Engineered where standard blocking buffers fail
Conventional 0.22 µm-filtered blocking buffers can carry subvisible particulates, inconsistent pH, and variable bioburden into sensitive immunoassays, elevating background noise and obscuring target signal. FluxMPS™ TBS Blocking Buffer with Gelatin (1%) is built to remove those failure modes at the source.
Microchannel-safe purity
Final 0.04 µm membrane filtration minimizes subvisible particulate carryover, supporting cleaner backgrounds in delicate microchannel and chip-based assay formats.
Precise, stable pH
Formulated to a defined pH of 7.4 with 50 mM Tris-Base and 150 mM Sodium Chloride, maintaining a consistent environment for protein and antibody integrity.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> quality expectations for sensitive assay reagents.
Low background for imaging & assays
The 1% gelatin matrix densely covers non-specific binding sites, improving signal-to-noise ratio for Western blot, ELISA, and IHC detection.
Defined, traceable composition
Every component — gelatin, Tris-Base, and Sodium Chloride — is disclosed at its exact working concentration with lot-based quality control.
Customization on demand
Alternate gelatin concentrations, additional salts, detergents, or pH adjustments can be formulated on request — contact support@diagnocine.com.
Quadruple-stage filtration system
DCP-GTBS1X is sterile-filtered through a redundant 0.1 µm membrane pass performed twice, followed by a 0.04 µm membrane pass performed twice, in a sterile environment — positioning purity well beyond a single 0.22 µm filtration pass.
-
1
0.1 µm Pre-filtration I
Large particulate and aggregate removal from the Tris-buffered saline base, extending downstream filter life.
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2
0.04 µm Pre-filtration II
Fine particulate and bioburden retention prior to final polish filtration.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm pass adds redundancy, reinforcing sterility assurance ahead of final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
A second 0.04 µm pass, performed in a sterile environment, provides the ultimate purity polish; this pore size is well below the smallest known mycoplasma-scale organisms, supporting mycoplasma-safe processing.
Performance vs. conventional buffer
Sequential 0.1 µm (twice) and 0.04 µm (twice) filtration removes finer particulates and bioburden than a single-pass 0.22 µm filtration typically used in conventional blocking buffers.
© Diagnocine® — DCP-GTBS1X
Where TBS Blocking Buffer with Gelatin (1%) is used
Formulated to block non-specific binding sites on membranes and microplates, DCP-GTBS1X supports a range of immunoassay and microfluidic assay workflows.
Automated Bioreactors & Robotics
For automated immunoassay platforms and robotic liquid handling, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available to further reduce particulate load on sensitive valves and sensors.
- Total Particulate Exclusion: sub-10 nm filtration removes particulates that standard 0.1/0.04 µm stages may pass
- Valve & Sensor Protection: minimizes particulate-driven wear on automated fluidic hardware
- Extended Perfusion Stability: reduces particulate accumulation over long automated run times
Inquiry Required: the 0.01 µm ultra-filtered grade is available on request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip Immunostaining
Blocks non-specific binding sites within on-chip immunostaining chambers, supporting cleaner detection signal in miniaturized formats.
Wash, Dilution & Reconstitution
Tris-buffered saline base with defined pH and ionic strength supports gentle wash and dilution steps ahead of blocking and detection.
iPSC-Derived Model Immunostaining
Reduces non-specific antibody binding when staining iPSC-derived neuron, cardiomyocyte, and hepatocyte cultures.
Endothelial & Primary Cell Immunolabeling
Supports background-free immunolabeling of endothelial and primary cell cultures used in perfusion-based vascular models.
ELISA, Western Blotting & Blocking
The 1% gelatin matrix blocks unoccupied binding sites on membranes and microplates, reducing background and improving assay signal-to-noise.
Microscopy & Optical Detection
Low-background blocking supports clearer immunofluorescence and chromogenic detection in microscopy-based readouts.
Physical, chemical & quality parameters
Specifications below reflect the values stated for DCP-GTBS1X; parameters not measured for this product are omitted rather than estimated.
| Parameter | Specification |
|---|---|
| Appearance | Clear, Colorless Liquid |
| pH | 7.4 |
| Tris-Base Concentration | 50 mM |
| Sodium Chloride Concentration | 150 mM |
| Gelatin Concentration | 1% |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase Activity | None detected (18 hr, room temperature, plasmid DNA) |
| RNase Activity | None detected (18 hr, room temperature, ribosomal RNA) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facility |
| Fill Environment | ISO Class 5 (Class 100) aseptic fill |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C (long-term storage: -20°C) |
| Shelf Life | 6 months |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485 | ISO 13485-certified facility (DiagnoCine Precision suppliers) |
| Regulatory Alignment | CE-approved facility |
| Production & Assembly | Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; custom assembly completed at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO); not for clinical, diagnostic, or therapeutic use in humans |
Full composition
TBS Blocking Buffer with Gelatin (1%) is a defined, lot-released Tris-buffered saline formulation blended with gelatin blocking protein. Every component below is disclosed at its exact working concentration.
| Component | CAS Number | Concentration |
|---|---|---|
| Gelatin | 9000-70-8 | 1% |
| Tris-Base | 77-86-1 | 50 mM |
| Sodium Chloride | 7647-14-5 | 150 mM |
Manufacturing & compliance
DCP-GTBS1X is manufactured, tested, and packaged under a controlled quality system spanning raw material sourcing through final fill.
ISO 13485:2016 QMS
Produced under ISO 13485-certified and CE-approved supplier facilities within the DiagnoCine Precision network.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) to minimize ionic and organic background.
ISO Class 5 Fill & Finish
Final sterile filtration and fill are performed in an ISO Class 5 (Class 100) controlled environment.
Micro-Batch Precision
Final packaging, quality assurance, and testing are completed at the DiagnoCine R&D and Quality Testing Center, with custom assembly at DiagnoCine Precision, Totowa, New Jersey, USA.
Endotoxin Control USP <85>
Endotoxin levels across the DiagnoCine buffer manufacturing pipeline are controlled per USP <85> Bacterial Endotoxins Test principles.
Particulate Control USP <788>
Sub-0.1 µm and sub-0.04 µm redundant filtration is designed to minimize particulate load consistent with USP <788> Method 2 principles.
Osmolality Reference USP <785>
Buffer formulations are developed with reference to USP <785> osmolality testing principles.
Documentation / CoA
A Certificate of Analysis documenting the manufactured lot is available for this product.
How DCP-GTBS1X compares
A side-by-side view of DCP-GTBS1X against conventionally filtered gelatin blocking buffers.
| Parameter | DCP-GTBS1X (FluxMPS™) | Conventional Blocking Buffer (0.22 µm filtered) | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Redundant Sub-0.1 µm Filtration Architecture | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Gelatin Blocking Formulation (1%) | check_circle | cancel | cancel |
| DNase / RNase Tested | check_circle | cancel | cancel |
| Water Quality (Ultrapure Type 1) | check_circle | cancel | cancel |
| Manufacturing QMS (ISO 13485) | check_circle | cancel | cancel |
| Microfluidic / OoC Channel Compatibility | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-GTBS1X TBS Blocking Buffer with Gelatin (1%).
Supporting literature
Curated references relevant to gelatin blocking chemistry, Tris-buffered saline, and organ-on-a-chip immunoassay applications.
- Huh D, Matthews BD, Mammoto A, et al. Reconstituting organ-level lung functions on a chip. Science. 2010. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Kurien BT, Scofield RH. Western blotting. Methods. 2006. doi:10.1016/j.ymeth.2005.11.007
- Good NE, Winget GD, Winter W, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Ferguson WJ, Braunschweiger KI, Braunschweiger WR, et al. Hydrogen ion buffers for biological research. Anal Biochem. 1980. doi:10.1016/0003-2697(80)90405-3
- Ramadan Q, Zourob M. Organ-on-a-chip engineering: toward bridging the gap between lab and industry. Biomicrofluidics. 2020. doi:10.1063/5.0011583
- Crowther JR. The ELISA Guidebook. Methods Mol Biol. 2009. doi:10.1007/978-1-60327-254-4
- Bass JJ, Wilkinson DJ, Rankin D, et al. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017. doi:10.1111/sms.12702
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