Cat No.: DCP-GTBS1X Size: 100 mL, 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
TBS Blocking Buffer with Gelatin (1%) is a reagent used in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry, to block non-specific binding sites on membranes and microplates. This helps enhance the specificity and sensitivity of the assays by reducing background noise. This buffer contains 1% gelatin, which is derived from animal collagen. Gelatin is effective at forming a gel-like matrix that covers potential non-specific binding sites. Gelatin is used to block unoccupied binding sites on membranes or microplates. This prevents antibodies or other detection molecules from binding randomly to the surface, which can lead to high background noise and obscure the detection of target antigens. By effectively blocking non-specific sites, gelatin can enhance the signal-to-noise ratio of the assay, leading to clearer and more reliable results. Tris-Buffered Saline (TBS) provides a stable pH environment, essential for maintaining the integrity of proteins and antibodies during assays. TBS is particularly beneficial in applications involving alkaline phosphatase detection systems, as it avoids phosphate interference.
* Not Suitable for All Membranes: Gelatin blocking buffer may not be suitable for PVDF membranes and is better suited for nitrocellulose, nylon, and charge-modified nylon membranes. While gelatin can be effective, its performance may vary depending on the specific assay conditions and the quality of the antibodies used.
Applications
Western Blotting: Used to block non-specific sites on membranes, ensuring that antibodies bind specifically to target proteins.
ELISA (Enzyme-Linked Immunosorbent Assay): Reduces background noise by preventing non-specific interactions between assay components.
Immunohistochemistry (IHC): Prevents non-specific binding of antibodies to tissue sections, improving the clarity and accuracy of staining.
Advantages:
Effective Blocking: The high concentration of gelatin provides a dense blocking layer that minimizes non-specific binding, leading to clearer and more accurate results. This helps enhance the sensitivity and specificity of the assays by reducing background noise.
Versatility: Gelatin blocking buffers can be used on various types of membranes, including nitrocellulose and nylon, although they are not suitable for PVDF membranes.
Compatibility with Detection Systems: Gelatin blocking buffer is compatible with a variety of detection systems, including biotin, fluorescein, and DIG detection systems. This makes it versatile for different types of assays.
Composition:
Ingredients
Concentration
Gelatin
1%
Tris-Base
50 mM
Sodium Chloride
150 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃ (For long term storage, store product at -20°C.)
Shelf life: 6 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
