PBS Blocking Buffer with Gelatin (1%)

Q: Is DCP-GPBS1X suitable for organ-on-a-chip (OoC) and microfluidic applications?

Its ultra-filtered, quadruple-stage purity (0.1 micron x2 plus 0.04 micron x2) and defined phosphate-buffered formulation make it well suited to low-background blocking and rinsing steps within microfluidic and organ-on-a-chip (OoC) immunoassay workflows, alongside its established use in Western blotting and ELISA.

Q: What makes the filtration superior to standard 0.22 micron-filtered buffer?

DCP-GPBS1X is filtered through a 0.1 micron membrane twice and a 0.04 micron membrane twice, a four-stage sequence that removes finer particulates and guards against mycoplasma contamination, since the smallest mycoplasma types can be about 0.2 micron, more thoroughly than a single 0.22 micron pass.

Q: Can DCP-GPBS1X be supplemented with salts, detergents, chelators, or other additives?

Yes. Alternate concentrations, additions of chemicals, compounds, proteins, or supplements, a different pH, or other modifications can be formulated on request.

Product Overview

ISO 13485 Certified Manufacturing

FluxMPS™ PBS Blocking Buffer with Gelatin (1%)

FluxMPS™ PBS Blocking Buffer with Gelatin (1%) is an MPS-grade, quadruple-stage ultra-filtered PBS-based blocking reagent formulated for Western blotting, ELISA, and other antibody-based immunoassays. Built on a stable pH 7.4 phosphate-buffered saline base with 1% gelatin, it forms an effective blocking matrix over non-specific binding sites on nitrocellulose, nylon, and charge-modified nylon membranes, supporting low-background detection across cell and molecular biology workflows.

Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice for mycoplasma-safe purity^[4]
Sterile, and confirmed free of detectable DNase and RNase activity after 18-hour challenge incubation
Stable pH 7.4 phosphate-buffered saline (PBS) base
1% gelatin blocking matrix, effective for protein-protein interaction blocking
Compatible with nitrocellulose, nylon, and charge-modified nylon membranes (not recommended for PVDF membranes)
Does not cross-react with mammalian antibodies
Manufactured under ISO 13485:2016-certified, CE-approved facilities
Customizable pH, concentration, and additive content available on request

SKU: DCP-GPBS1X UNSPSC: 12161706 Neutral buffers

PBS Blocking Buffer with Gelatin (1%) — Sterile, Ultrapure

pH7.4
Gelatin1%
Sodium Chloride137 mM
Potassium Chloride2.7 mM
Na₂HPO₄ (anhydrous)10.1 mM
KH₂PO₄ (anhydrous)1.76 mM
AppearanceClear, colorless liquid
Filtration0.1 µm x2 + 0.04 µm x2
Storage4°C (-20°C long-term)
Shelf Life6 months

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard blocking buffers fail

Conventional single-pass 0.22 µm-filtered blocking buffers can carry subvisible particulates, inconsistent pH, and residual bioburden into sensitive immunoassay and imaging workflows, raising background and reducing reproducibility. FluxMPS™ addresses each of these failure modes directly.

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Microchannel-safe purity

Quadruple-stage filtration through 0.1 µm and 0.04 µm membranes (each applied twice) delivers an ultra-low particulate blocking buffer suitable for demanding imaging and microfluidic workflows.

target

Precise, stable pH

Manufactured and quality-control tested to pH 7.4 on a defined phosphate-buffered saline base for consistent protein and antibody stability across assays.

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Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm), consistent with USP <85> water-quality expectations for sensitive reagent formulation.

visibility

Low background for imaging & assays

The 1% gelatin matrix effectively covers non-specific binding sites, improving sensitivity and specificity in Western blotting and ELISA detection.^[1,2]

science

Defined, traceable composition

Every lot is formulated to a fixed composition of five named components with concentrations released per lot — see the full composition table below.

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Customization on demand

Alternate concentrations, additional chemicals, compounds, proteins, supplements, or a different pH can be formulated on request.

Purity Architecture

Quadruple-stage filtration system

DCP-GPBS1X is sterile-filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice in a controlled sterile environment, giving a four-stage purity architecture designed to prevent mycoplasma contamination.

1

0.1 µm Pre-filtration I

First 0.1 µm membrane pass removes large particulates and gelatin aggregates, protecting the finer-pore membranes used downstream.
2

0.04 µm Pre-filtration II

First 0.04 µm membrane pass retains finer particulates and bioburden, further reducing potential background contaminants ahead of final filtration.
3

0.1 µm Sterile-filtration I

Second 0.1 µm membrane pass provides redundant clarification prior to the final polish.
4

0.04 µm Sterile-filtration II — Final Polish

Second 0.04 µm membrane pass performs the ultimate polish in a sterile environment, guarding against mycoplasma-scale contaminants — the smallest mycoplasma types can be about 0.2 micron.^[4]

Performance vs. conventional buffer

Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates and provides greater assurance against mycoplasma contamination than a single-pass 0.22 µm filtration used in conventional blocking buffers.

0.04 µm

Final filtration stage

4

Total filtration stages

Every lot is filtered through validated 0.1 µm and 0.04 µm membranes in a controlled sterile environment, then confirmed free of detectable DNase and RNase activity following 18-hour challenge incubations with plasmid DNA and ribosomal RNA at room temperature, respectively.

DCP-GPBS1X FluxMPS PBS Blocking Buffer with Gelatin quadruple-stage 0.1 micron and 0.04 micron filtration diagram for organ-on-a-chip and microfluidic immunoassay applications, Diagnocine — **Figure 1.** Quadruple-stage filtration architecture (0.1 µm x2 + 0.04 µm x2) used to manufacture DCP-GPBS1X.
© Diagnocine® — DCP-GPBS1X

Applications

Where DCP-GPBS1X is used

A gelatin-based PBS blocking buffer purpose-built for antibody-based detection assays and for downstream integration into microfluidic and organ-on-a-chip immunoassay formats.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer can be formulated on request for maximum system protection.

Total Particulate Exclusion — 10 nm-grade filtration for the most sensitive fluidic paths
Valve & Sensor Protection — reduces particulate load on precision automation components
Extended Perfusion Stability — supports longer unattended run times in closed fluidic systems

Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available by request — contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

Low-background blocking and rinsing buffer for microfluidic immunoassay formats.

OoCToCBoCLoCMPS

Sample Preparation

Wash, Dilution & Reconstitution

Stable pH 7.4 phosphate-buffered saline base suitable for wash and dilution steps in assay workflows.

DilutionRinse

Stem Cell Biology

iPSC-Derived Model Handling

Compatible with blocking and wash steps used in iPSC-derived model immunostaining workflows.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cell Perfusion

Ultra-filtered, defined-pH buffer suited for perfusion-adjacent wash steps with primary and endothelial cell models.

HUVECsHAECsPrimary hepatocytes

Immunoassays

ELISA, Blotting & Blocking

Blocks non-specific binding sites on membranes and microplates, reducing background in Western blotting and ELISA.^[1,2]

ELISAWestern blotIHCIF

Live-Cell Imaging

Microscopy & Optical Sensing

Low-particulate, defined-pH composition supports clean backgrounds in optical detection formats.

ConfocalBiosensorsTEER

Technical Specifications

Specifications at a glance

All values below are as manufactured and quality-control tested for DCP-GPBS1X.

Physical & Chemical Parameters

Parameter	Specification
Formulation / Composition	PBS with 1% gelatin — see the full composition table below
Appearance	Clear, colorless liquid
pH USP <791>	7.4
Sodium Chloride Concentration	137 mM
Potassium Chloride Concentration	2.7 mM
Na₂HPO₄ (anhydrous) Concentration	10.1 mM
KH₂PO₄ (anhydrous) Concentration	1.76 mM
Gelatin Concentration	1%

Sterility, Purity & Safety Parameters

Parameter	Specification
Sterility	Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment
DNase Activity	None detected (18 hr incubation with plasmid DNA at room temperature)
RNase Activity	None detected (18 hr incubation with ribosomal RNA at room temperature)
Filtration System	0.1 µm x2 + 0.04 µm x2 (quadruple-stage)
Water Quality	Ultrapure Type 1 water (18.2 MΩ·cm) USP <85>
Manufacturing Standard ISO 13485:2016	Certified, CE-approved facilities

Storage, Handling & Logistics

Parameter	Specification
Storage Temperature	4°C (short-term); -20°C for long-term storage
Shelf Life	6 months

Raw Materials & Regulatory Traceability

Parameter	Specification
Manufacturing QMS	ISO 13485:2016-certified facility
Regulatory Alignment	CE-approved facility (Suppliers of DiagnoCine Precision)
Production Location	Final packaging, quality assurance, testing, and customization performed at DiagnoCine Precision, Totowa, New Jersey, USA
Intended Use	Research Use Only (RUO); not for clinical, diagnostic, or therapeutic use in humans
Membrane Compatibility	Compatible with nitrocellulose, nylon, and charge-modified nylon membranes; not recommended for PVDF membranes

Formulation

Full composition

Five named components at fixed concentrations, released per lot.

Component	CAS Number	Concentration
Gelatin	9000-70-8	1%
Sodium Chloride	7647-14-5	137 mM
Potassium Chloride	7447-40-7	2.7 mM
Sodium Phosphate Dibasic Anhydrous	7558-79-4	10.1 mM
Potassium Phosphate Monobasic Anhydrous	7778-77-0	1.76 mM

CUSTOMIZATION: alternate concentrations, additions of chemicals, compounds, proteins, or supplements, a different pH, and other modifications can be formulated on request — contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

DCP-GPBS1X is manufactured, packaged, and tested under a controlled quality system.

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ISO 13485:2016 QMS

Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).

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Ultrapure Type 1 Water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> expectations.

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ISO Class 5 Fill & Finish

Final filtration and fill steps are performed under controlled aseptic conditions.

assignment

Micro-Batch Precision

All final packaging, quality assurance, and testing are completed at the DiagnoCine R&D and Quality Testing Center, with specific customization requests assembled at DiagnoCine Precision in Totowa, New Jersey, USA.

Endotoxin USP <85> BET

Endotoxin testing follows USP <85> Bacterial Endotoxins Test protocols as part of routine manufacturing quality control.

Particulate USP <788> Method 2

Particulate assessment follows USP <788> Method 2 as part of the filtration validation program.

Osmolality USP <785>

Osmolality testing is available within the USP <785> framework as part of the quality program.

Documentation / CoA

A Certificate of Analysis documenting appearance, pH, sterility, and DNase/RNase results is available on request.

To request a Certificate of Analysis for this lot, contact support@diagnocine.com.

Product Comparison

How DCP-GPBS1X compares

DCP-GPBS1X against conventional single-pass filtered gelatin blocking buffers.

Parameter	DCP-GPBS1X (FluxMPS™)	Conventional Buffer (0.22 µm filtered)	Standard Alternative (0.22 µm filtered)
Filtration Architecture	0.1 µm x2 + 0.04 µm x2 (4-stage)	Single 0.22 µm pass	Single 0.22 µm pass
DNase / RNase Validated	check_circle	cancel	cancel
Gelatin-Based Blocking Formulation	check_circle	cancel	cancel
Stable pH 7.4 Phosphate-Buffered Base	check_circle	check_circle	cancel
Water Quality	Ultrapure Type 1 (18.2 MΩ·cm)	Standard purified water	Standard purified water
Manufacturing QMS	ISO 13485:2016	cancel	cancel
Membrane Compatibility Guidance	check_circle	cancel	cancel
Custom Formulation Available	check_circle	cancel	cancel

FAQ

Frequently asked questions

Common questions about DCP-GPBS1X.

Its ultra-filtered, quadruple-stage purity (0.1 µm x2 + 0.04 µm x2) and defined phosphate-buffered formulation make it well suited to low-background blocking and rinsing steps within microfluidic and organ-on-a-chip (OoC) immunoassay workflows, alongside its established use in Western blotting and ELISA.

DCP-GPBS1X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice — a four-stage sequence that removes finer particulates and guards against mycoplasma contamination (the smallest mycoplasma types can be about 0.2 micron) more thoroughly than a single 0.22 µm pass.^[4]

DCP-GPBS1X is formulated at pH 7.4 on a PBS base of 137 mM sodium chloride, 2.7 mM potassium chloride, 10.1 mM sodium phosphate dibasic anhydrous, and 1.76 mM potassium phosphate monobasic anhydrous, plus 1% gelatin. Alternate concentrations, a different pH, or added components can be formulated on request — contact support@diagnocine.com.

The product is quality-control tested to pH 7.4; a specific measurement temperature is not stated in current specification documentation for this lot. For stability, store at 4°C for short-term use and -20°C for long-term storage, within the 6-month shelf life.

Yes. Please inquire if other concentrations, additions of chemicals, compounds, proteins, or supplements, a different pH, or other modifications are needed — contact support@diagnocine.com.

A specific endotoxin value is not stated in current documentation for this lot. Purity is instead demonstrated through sterility filtration and confirmed absence of detectable DNase and RNase activity after 18-hour challenge incubation. Contact support@diagnocine.com for the current Certificate of Analysis.

Yes. The CoA documents appearance, pH, sterility filtration confirmation, and DNase/RNase testing results. Request it at support@diagnocine.com.

Scientific References

Supporting literature

Curated references relevant to blocking buffer chemistry, immunoassay methodology, and microphysiological systems.

Kurien BT, Scofield RH. Western blotting. Methods. 2006;38(4):283-293. doi:10.1016/j.ymeth.2005.11.007
Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Immunochemistry. 1971;8(9):871-874. doi:10.1016/0019-2791(71)90454-X
Mansfield MA. Rapid immunodetection on polyvinylidene difluoride membrane blots without blocking. Anal Biochem. 1995;229(1):140-143. doi:10.1006/abio.1995.1146
Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75-90. doi:10.1023/A:1022913015916
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772. doi:10.1038/nbt.2989
Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662-1668. doi:10.1126/science.1188302
Kim HJ, Huh D, Hamilton G, Ingber DE. Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. Lab Chip. 2012;12(12):2165-2174. doi:10.1039/c2lc40074j
Bass JJ, Wilkinson DJ, Rankin D, et al. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017;27(1):4-25. doi:10.1111/sms.12702