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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-BRN1X
$44.00
DCP-MEMH-BRN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Low Glucose (1.0 g/L) MEM base with 25 mM HEPES; bicarbonate-free and phenol-red-free for reduced CO2 dependence and low optical background
  • Quadruple-stage filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), controlled per manufacturing batch
  • pH 7.4 (USP <791>); prepared with Type 1 water (18.2 MΩ·cm)
  • L-Glutamine 292 mg/L and Sodium Pyruvate 110 mg/L included directly in the base formulation
  • Manufactured under an ISO 13485:2016 quality management system; ISO Class 5 aseptic fill & finish
  • Microfluidics Suitable (0.04 µm final cut-off) — built for organ-on-a-chip, tissue-on-a-chip, and microphysiological system (MPS) platforms
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMH-BRN1X |Sizes: 500 mL, 1000 mL |Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-Glutamine292 mg/L
  • Sodium Pyruvate110 mg/L
  • HEPES5958 mg/L (25 mM)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and inconsistent particulate loads that accumulate inside microchannels — risking biosensor signal drift and shortened device lifetimes. FluxMPS™ is built to reduce these risks at the source.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 particulate compliance verified per batch.

target

Total metabolic control

Defined Low Glucose, L-glutamine, and sodium pyruvate concentrations for metabolic flux experiments and Warburg-related research.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) for low trace-metal and organic-carbon (TOC) content.

visibility

Low background for imaging

Low particulate density from quadruple-stage filtration reduces background scatter — suited to confocal microscopy, live-cell biosensors, and TEER measurements.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid is processed through a four-stage serial filtration sequence — two dedicated prefilter/final-filter pairs — reaching a 0.04 µm final cut-off.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including organisms in the mycoplasma size range (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge from breakthrough.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 (light obscuration) compliance verified per batch.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every batch undergoes 14-day USP <71> sterility testing. Mycoplasma is addressed by 0.1 µm mycoplasma-retentive filtration during Prefiltration I & II; this is filtration-based risk mitigation, not a per-lot USP <63> mycoplasma detection assay.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid (DCP-MEMH-BRN1X) Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-BRN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid is suited to organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and lot-to-lot variability are undesirable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 0.01 µm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: reduces particulate-induced blockage risk in precision fluidic systems
  • Extended Perfusion Stability: supports flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the MPS Grade 0.01 µm variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports microchannel integrity and laminar flow in chip-based systems.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined Low Glucose base with controlled sodium pyruvate enables precise metabolic flux analysis.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered, low-particulate formulation supports sensitive iPSC differentiation protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement studies.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

Defined carbon and nitrogen sources support 13C isotope tracing for metabolic flux analysis. Bicarbonate-free and phenol-red-free formulation is compatible with Agilent Seahorse XF assays.

13C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load from quadruple-stage filtration supports confocal imaging and biosensor integration with reduced background scatter.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production batch of FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Contains L-Glutamine, HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate; without Sodium Bicarbonate, Phenol Red
Appearance Pale-yellow colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine 292 mg/L
Sodium Pyruvate 110 mg/L
Phenol Red Not present
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Compliant (Method 1)
Particulate ≥25 µm USP <788> Compliant (Method 1)
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement HEPES-buffered; reduced CO2 dependence (bicarbonate-free formulation; validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Sodium Pyruvate 113-24-6 110.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production batch.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water (ASTM Type I / ISO 3696).

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay per batch (assay sensitivity 0.005 EU/mL). Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per batch.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEMH-BRN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-MEMH-BRN1X (FluxMPS™) Conventional MEM (0.22 µm) Standard MEM alternative
Grade Microfluidics Suitable Standard grade Standard grade
Base Formulation Contains L-Glutamine, HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate; without Sodium Bicarbonate, Phenol Red MEM Standard MEM Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. This Microfluidics Suitable medium uses our quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that reduce the risk of microchannel clogging in OoC, ToC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or the subvisible particulates that accumulate in microchannels. This medium is processed through four sequential stages reaching a 0.04 µm final cut-off, delivering approximately 5× cleaner media by particulate count per USP <788> Method 1 (light obscuration).
This formulation contains Low Glucose at 1.0 g/L (1000 mg/L), plus L-Glutamine (292 mg/L) and Sodium Pyruvate (110 mg/L) already in the base formulation. Contact support@diagnocine.com for a custom glucose concentration rather than adding additional glucose, glutamine, or pyruvate on top of what is already present.
This formulation is bicarbonate-free and HEPES-buffered (25 mM), giving reduced CO2 dependence; validate the appropriate CO2 level for your specific cell line. Standard CO2 incubation remains compatible if preferred.
Yes. This medium can be supplemented with FBS (typically 5–20%), growth factors, antibiotics, or other additives per standard practice. Filter serum-containing supplements through a 0.2 µm low-protein-binding PES or PVDF filter before addition; the 0.04 µm final filtration used in this medium is not appropriate for filtering serum or protein-containing additions.
Endotoxin is controlled per manufacturing batch by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL). Release specification: < 0.05 EU/mL. Batch-specific results are provided in the CoA, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788>), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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